MicrobeHunter.com Microscopy Forum

One thing that I have spotted is a bit of corrosion on some of the screws. The objectives are crusty as well. As things on ebay tend to go bad rather than good and given the shabby appearance of the scope I'd make a guess and say it doesn't look like a particularly good deal Perhaps an unpopular op...

Not sure about your ebay predicament but got curious about the object of desire in this dodgy transaction

I like this thread! For many reasons, this type of periodontal clinical microscopy died out in the 1980's. I'm sure there are still many weird organisms living in there, unknown to science, but no one is looking for them any more. But why would people willingly abandon such a research niche? Seems i...

Thats a cool channel that one can easily slip into binge watching. They use a well slide in this section but I suppose if you want to do this properly i.e. quantitatively I'd suggest you hydrolyse the biological material with hydrochloric acid, then centrifuge to collect the plastic bits and stain w...

What you need is a Zeiss West 43.5 mm dovetail to either a male M42 or T2 adapter (see picture below). What worked for me was to add a M42 female to 52 mm male thread adapter but that could be any thread your camera lens accepts. As Bob had advised me at some point I bought a Canon 40 mm pancake len...

Interesting test, thanks. I am working on plotting glass-water-glass transmission vs. NA (somewhat separate issue from aberrations) as mentioned in the other thread but not finished yet. Might be useful to try to separate effect of watering the condenser vs. objective in this sort of experiment? I ...

Yes and I can't recommend one more. Absolutely fantastic, totally worth the 200 or 300 bucks I paid for it (came with a fancy HEPA filter and whatnot). I've been thinking about getting a de-humidifier as well, seems like a logical extension of my efforts in environmental control (the purifier actual...

This is some pretty high end imaging for a microscope without condenser. Thanks for posting.

Now that is a well controlled comparison. Thanks for posting Hobbyst. Number 11) looks like a top of the line homogenous immersion system, really nice and crispy. I only have the universal condenser D position darkfield option at my disposal and I've used it with water which produced ok results at l...

Great video with really nice detail from the DIC. Thanks for sharing.

Very morbid, interesting nontheless. I find it cool that you can link a specific crime scene locale to the species identified during a forensic pathology investigation.

I use water instead of oil on the condenser for high NA objectives, its a decent compromise (according to my math it allows a cone of light with NA=1.2) ... Is that number less than 1.33 because it is based on some criteria like 50% transmission rather than critical angle? I never said I was good i...

- I read Abbe condensers have to be oiled with higher than NA 1.0 objectives; is that so? can all abbe condensers be oiled? - Do you need an apo condenser to use high end apo lenses? like those 60/1.40 Oil ones.. I use water instead of oil on the condenser for high NA objectives, its a decent compr...

I picked up a used Canon T3i camera recently and have been having some fun adapting it to my scope and taking some shots. Live View through the computer monitor with the Canon utility software sure is handy. But I worry about overheating the imaging sensor. I read all kinds of conflicting informati...

Interesting, where did you find them?

Really cool amoeba you found there! Thanks for posting.

btw what setup are you using?

Excellent as always

Nice finding, very interesting protists!

These are really good images. Ascorbic acid is great as Greg mentioned, just make sure its the acid not the sodium salt (sodium ascorbate) which really doesn't want to crystalize. Also a personal favourite of mine is acetaminophen (known as paracetamol in Europe and tylenol in the states), its readi...

These are amazing! Beautiful diatoms and impeccable imaging. Very well done and thank you for posting.

Hi Dawn,

Congratulations on your new scope. We might be able to tell you what scope you got if you post pictures here.

BR
Wes

Observed strange structures inside an amoeboid. Appear small and round in size, very motile but restricted to a vacuole-like structure. Anyone seen anything like that?

Hi Michael, I once attempted to write a post about the nature and use of wave plates. You can find it here: https://www.microbehunter.com/microscopy-forum/viewtopic.php?f=5&t=8197&p=71875#p71875 In short the retardation plate is a piece of optically anisotropic material that exhibits direction-depen...

Great detail. You should really consider mounting these guys as you have a great source of material right under your nose.

Good video, looks like Stauroneis.

Hey all. I have a 3d printer and I've run out of things to make. If anyone needs something done up, I'm game to give it a go. This would be useful: https://github.com/beniroquai/Tensorflow_Jamin-Lebedeff/tree/master/STL Its a set of parts to build an analyser (rotating polarizer). The compensator a...

Now thats really nice shot. Excellent work!

To quote the CDC: Although most cases of primary amebic meningoencephalitis (PAM) caused by Naegleria fowleri infection in the United States have been fatal (144/148 in the U.S., 1), there have been five well-documented survivors in North America: one in the U.S. in 1978 2, 3, one in Mexico in 2003...

Excellent footage! What optics are you using?

viktor j nilsson wrote: ↑

Thu Feb 11, 2021 9:22 pm

Btw, how do you dry the cover glass after the piranha bath?

Multiple washes with deionized water, then submerge the coverlips under ethanol. Whenever I need a coverslip I use tweezers to grab one and let the ethanol evapore on its own, it doesn't leave traces if its sufficiently clean.