MicrobeHunter.com Microscopy Forum

Hi together, I tried to make a comparison to get a better understanding about how much Zeiss DIC "alt" can manage in fine detail compared to other illumination methods. The objective was a Leitz NPL Fluotar 100:1 n.a, 1,32 which works, but with some colour gradient. Each stacked from dozens of image...

That is an interesting aspect! I knew that the image changes this way, but I didn't know that it is just this small difference. And this is really a challenge with placton photograpy - maintain a thin layer to get good images and keep it thick enough for the bigger specimens to survive. If one would...

Hi Glen,
what do you mean with bias and what did you do with the cover slip?

Bob

I took these photos with my Nikon 1J5 that is directly connected to an older Leitz Periplan objective and 8mm spacer to come to a 10mm eyepiece pickup point. I just plunged the camera in the bino tube an can rotate it if necessary to get the object in the frame. Right now I have mounted a rectangula...

Here a very long diatom, several hundered µ.

Hi together, I found some more time to look through Doron's Pleurax.mounted slide. It is always astonishing, how long one can look at a strew slide and still found new diatom forms sponge needles and other interesting oblects. Here a fragment od a beautilful diatom, there was just this single one so...

The object guide on the rotating stage of my 1911 "Großes Forschungsmikroskop" already has a vernier scale, as finely engraved as if it were a measuring tool.

As far as I know the stated values have to be set at the scales of the object guide on to of a rotating polarisation microscope stage. It is intended for the microscope table with the stated number and was sold together with it. By using it you can quickly center the stage when necessary. "Nonius" m...

This is how a typical phase telescope looks: https://www.ebay.de/itm/Hilfsmikroskop- ... 2752640117
In this case a Zeiss Jena, not Zeiss West.

Hi Michael, the phase telecope should give you a sharp view of the phase rings. First adjust the focus to the slide and the condenser and field aperture to Köhler condition. If it doesn't focus it might not be a phase telescope at all or lenses are missing or are mixed up inside. These are simply bu...

Hi Glen, thank you for checking this. The 0,9 top lens is nowhere mentioned in combination with DIC "alt" but it might nontheless work very well. As far as I know the dic prisms limit the n.a. of the condenser to 0,9 anyway so there may be little actual loss. I plan to design a 3D-printable box for ...

75RR wrote:Here is some info on the 465277 condenser model:

Do you have information about the top lenses that were available? I have a n.a. 1,4 and 0,63 but there may have been a 0,9 too. This could be useful to find other working DIC combinations.

Bob

For a conical net the material is a bit expensive, but for filters made from plastic tubes and stainless mesh it is ideal. The mesh can simply be welded onto the tube with any piece of metal that has more than the melting temperature of the plastic. For plancton fishing one can just pour 20 beakers ...

I also wrote a post where I describe the overhaul of these condensers: http://www.microbehunter.com/microscopy-forum/viewtopic.php?f=5&t=7850 I'm quite sure that this will help you through the repair job. If you can repair a bicycle, you can repair the condenser. From the repair point it is not muc...

Hi together,

for comparison I show here a picture of the stainless steel mesh I like to use. It is either 25µ or 50µ.
Compared to the plastic fibre net material it is more precisely woven and quite robust.

Bob

MichaelG. wrote:Is it presumed [or known] that the lens in the 45 60 29 adapter is the same one as in the MC63 ?

I don't know. I have had no intention to use a full format camera and for this reason I have never looked closer at these original Zeiss film camera adapters.

Hi Michael, thank you for the brick wall test! There was a very interesting thread about this photo adapter in the german mikroskopie-forum.de: https://www.mikroskopie-forum.de/index.php?topic=23644.0 The conclusion is that the 63mm lens is not really up to the performance of today's digital sensors...

In any case stain(s) and mountant will have to fit together. The mountant shouldn't draw out the stain and the ph value of the mountant has to fit to the stain so it doesn't loose its colours over time. For plant sections FCA Etzold or W3A Wacker stain are proven and work well with Euparal mountant....

I like your idea with the lead - tape. This solves the problem to apply weight in well spread manner to the sensitive mesh.

I have no idea what the “intermediate tube, the top lenses and the DIC analyser” look like and I haven’t found much documentation on line. Hi Michael, here is a link to my document: https://drive.google.com/open?id=1z5XreWeTZiPwkz-7l6OpMCVBgcIoydXF For now it's only in german, but I will make an en...

That ebay -Junior is a fairly new "Chromleisten-Junior" - it has different guides for the stage, probably roller bearings like the later Standard RA ... The chromed guides are steel, but to me the base of the arm looks much like aluminium. This would also fit to the anodized look of the tube port. T...

Hi Glen, I had a look at the tube port of an old Standard Junior and an old Zeiss-Winkel GFL. Both have a brown anodized looking surface. From the look of the slightly worn edge this could even be aluminium. From the weight it is not really feasible that there is much aluminium in the construction. ...

Hi Michael, these condensers are both really nice, incredibly well made and optically very good. I can send you a german text I have assembled recently about setting up old Zeiss DIC systems. I also wrote a post where I describe the overhaul of these condensers: http://www.microbehunter.com/microsco...

Hi Phil,
I have made two phase rings from sheet metal discs, held together with a wire that is glued on top. I think I used 0,3mm aluminium and punched it out with a high quality hole punch set. From there a bit of manual work. It's been a while since but I think they were quite precise.

Bob

Hi Glen,
are you sure it is made of brass? My impression was that most cast microscope stands of this era were made from cast iron.

Bob

Clearly over and in love with the improved smell in the lab-kitchen! I think I will switch over to a clear variant of liquor for a more professional look

75RR wrote: I am going to assume the DW means Distilled Water - hope I am right!

Oh no - I just tried it with diluted wiskey!

apochronaut wrote:...and I now am in the latter stages of finishing off a petite 4 objective binocular...

Hi Phil,
I'm looking forward to see this instument when it is finished!

Bob

Hi Mike, this is a standard WL, an eary black one. Zeiss know their numbers but I don't think you can just say this number - this year. Zeiss has an archive and they may be willig to answer the question: https://www.zeiss.de/corporate/ueber-zeiss/geschichte/archiv.html I haven't contacted them so fa...

Hi Michael, the middle two materials offer quite regular mesh sizes. I like stainless steel mesh better for sieves: Stronger, more precise, easy to weld to a ring and easy to clean. These nets can probably cause trouble when you sample in sensitive environments since they tend to keep part of the ca...