MicrobeHunter.com Microscopy Forum

One example quoted states that a 15x eyepiece with a x40 objective gives 600x magnification while still providing acceptable resolution. Since I have no experience using either, I wanted to ask members what the experience is like with using higher power eyepieces when compared to using oil immersio...

My point was not that Swift weren't Made in Japan rather, it was whether they had manufacturing facilities there. In other words did Swift ever own a microscope factory, as is more or less claimed by the current owners of the trademark, or did they simply have an existing company, make scopes for t...

Here is the manual for the Swift M3200 ... printed in Japan
https://www.swift-microscopeworld.com/I ... Manual.pdf

Admittedly not a guarantee that the instrument was made there; but a substantial hint.

MichaelG.

Interesting how some companies likes to put a spin on things. The Texas website explicitly states " for over fifty years Swift Instruments has been a global leader in the manufacture and production of quality microscopes", when in fact it sounds like the current company does not manufacture but the...

The monochrome image is a beauty !!

MichaelG.

Thank you! I will go try it now. If I am unable to find that "one match" is there a way to spot it? Or is it a process of feel? Just a matter of feel ... Count how many starts there are; then pick one at random: If it doesn't feel good just back-out, keep a little pressure on, and and let it drop i...

That looks like a multi-start left hand thread.

There will probably be one 'match' that works slightly better than the others, but it should just screw in anti-clockwise.

MichaelG.

Wonderful work, John
... Sorry, I missed the opening post on Monday.

Oh for a Scanning Electron Microscope !!

MichaelG.
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They do look rather like Espresso Machines, but ...
http://www.phenom-world.com/microscopes
A nice man showed me the Pro X version, but I would be happy to have any of them.

There are books specifically for the industry, but I don't have a copy of any and cannot remember any titles. A web search may offer some suggestions. lorez Thanks for that ... I will have a look around. MichaelG. . . These look like good places to start: http://www.biologicalwasteexpert.com/useful...

Whatever critters disappear are always magically replaced with others, I do a lot of work with wastewater treatment plants and this is the hallmark of their evaluations. When a certain species is present they know a certain condition exists. lorez That must be an interesting exercise ... Is there a...

Gorgeous images !!

MichaelG.

I'll welcome any suggestions on ways to compare the corrections, I have pretty much just been flailing about on my own (obviously). ... The classic visual test of Achromatism seems to be Carpenter's "Disc of Deal" Sorry ... I haven't had a chance to try it yet. MichaelG. . . https://archive.org/det...

Excellent result, John [in both senses]

'fraid I can only currently see your first image though.

MichaelG.

The optimum technical solution would, I think, be a square format sensor

But, as PeteM mentions; viewing formats are typically rectangular, and tending towards wider aspect ratios.

Given square pixels: Circular sensors are really not a credible solution.

MichaelG.

A Cornucopia, no less

What a delightful picture and good sentiment.

MichaelG.

Very beautiful images

MichaelG.

Tried just about every combination of 'scope and condenser etc but no joy. Knowing how superb the Leitz 'scopes are it seems clear that the answer would be the fabled 'large-field condenser' which I suspect will be very hard to find indeed. I feel your pain, John Page 15 of the Ortholux II manual h...

Sorry but this is way out of my league ... My best suggestion is to have a look at https://micro-manager.org There is an active forum; and I do recall seeing a very impressive demo of mosaic acquisition, by Märzhäuser, using that software. Do please let us know how you get on with this interesting p...

Great images !!

MichaelG.

Its a special condenser to this objective

Excellent !!
Thanks for posting the photo.
Do you know [even approximately] the focal length?

MichaelG.

The Orthoplan's condenser (600 series lower element and body 'bathtub') fills the x4 objective fine with the top lens swung out, but I suspect the x1 objective may need the condenser out as well... At the risk of labouring this, John [a foolish thing for me to do, given that I have never used the o...

p.s. do you think it may work for whole tissue (i.e. not flat and sectioned) with incident illumination too to some extent? That would be very useful also. Yes, I think it should be fine. You may, however, find it useful to experiment with lighting arrangements; because there is quite a high risk o...

Ah, yes I see Michael, will closing the iris raise contrast also, which can be handy at such a low magnification I think? Probably not [much], John ... but you may find that closing it just a little does improve things [as it does on many camera lenses]. That said: One would hope that Leitz careful...

Aha Michael - yes, I see - erm... what does one do with the built-in iris diaphragm. :oops: Sorry that's a stupid question but I've never encountered an objective with this feature. How is it used in practice Michael? Full aperture will give you the highest resolution, and the brightest image, but ...

John, I stand to be corrected, but I am sure that 'built-in iris diaphragm' is not the same thing as 'correction collar' ... I think you will find that the diaphragm serves exactly the same purpose as the one in a camera lens. I have never seen a low power objective that needed a correction collar. ...

Thank you ... that clarifies nicely. The main reason that I asked was because I could see no reference to an eyepiece. >> It is now clear that you did not use one ... I also did not use the eyepiece in the home made camera mount tube. ... which makes the performance of the objective all the more imp...

That looks very promising indeed !!

Some technical details of the set-up would be appreciated.

MichaelG.

I'm pretty sure that you are right, Pat
... see my note [Wednesday] on your other thread.
viewtopic.php?f=6&t=5450

It certainly got me thinking though !!

MichaelG.

Thumbnail version

And there is also some light on the question left from the previous thread . It is not evident while the rotifer is contracted, but at 2:20 the one shows a round bubble at the cloaca, which then abruptly contracts and disappears. Thanks for that observation ... Your analysis makes good sense. Micha...