Search found 977 matches

by dtsh
Wed Jun 14, 2023 2:43 am
Forum: Pictures and Videos
Topic: Newbie learns to take images, dtsh edition
Replies: 66
Views: 18652

Re: Newbie learns to take images, dtsh edition

Another try, this time with the illumination turned down.
Stellaria.media.pollen.jpg
Stellaria.media.pollen.jpg (46.85 KiB) Viewed 767 times
by dtsh
Tue Jun 13, 2023 10:00 pm
Forum: My microscope
Topic: My Leeuwenhoek homage microscope
Replies: 6
Views: 2219

Re: My Leeuwenhoek homage microscope

Nicely done and I suspect it's got a bit more eye relief than the originals, too.
by dtsh
Tue Jun 13, 2023 9:29 pm
Forum: Pictures and Videos
Topic: Newbie learns to take images, dtsh edition
Replies: 66
Views: 18652

Re: Newbie learns to take images, dtsh edition

It's been a while since I've done much playing around with imaging, but I'm trying to test out a new LED refit for the AO10 and decided to play with some darkfield for a bit. Subject is pollen from Stellaria media "Chickweed" at 1000x, stack of 33 images. I need to work with some of the settings, th...
by dtsh
Mon Jun 12, 2023 2:21 pm
Forum: Pictures and Videos
Topic: pieris scales
Replies: 10
Views: 2062

Re: some scales

Thanks for the anaglyph image!
by dtsh
Sat Jun 10, 2023 12:16 am
Forum: Home-made microscope adaptations
Topic: American Optical Series 10 LED replacement for 1036A
Replies: 33
Views: 7417

Re: American Optical Series 10 LED replacement for 1036A

I wasn't happy with the images I took, so I ended up assembling another one. Here is an image of the XL6019 and potemtiometer assembly soldered up, ready for testing. AO10_drop-in_XL5019_ass.jpg Here it is temporarily assembled for testing. Note, the switch built-in into the potentiometer isn't conn...
by dtsh
Thu Jun 08, 2023 4:34 pm
Forum: Pictures and Videos
Topic: Asian hornet
Replies: 6
Views: 839

Re: Asian hornet

If my understanding is right, the antenna are from a female? I'm not familiar with this species but other related species can be identified by the number of segments in the antenna flagellum. This one looks to have 10, so I think it would be female.
by dtsh
Tue Jun 06, 2023 6:54 pm
Forum: Home-made microscope adaptations
Topic: American Optical Series 10 LED replacement for 1036A
Replies: 33
Views: 7417

Re: American Optical Series 10 LED replacement for 1036A

Here's where we get to the gross parts where I do things in a very unprofessional manner. I'm being extra verbose here because when I first tried this sort of thing a few years ago I would have appreciated some extra notes for someone who wasn't experienced with building circuits (I'm still not, but...
by dtsh
Mon Jun 05, 2023 1:45 am
Forum: Pictures and Videos
Topic: Rat tapeworm egg in dog feces
Replies: 5
Views: 791

Re: Rat tapeworm egg in dog feces

Hi Dennis Well for the dog...it pays to have a doctor in the family ! Even better that I have a rason or excuse for my hobby, ...hey wife.. "I need more time and microscopes because I need to examine the feces of the dog" :-) Hi dtsh, I also check fecal samples from time to time with the various an...
by dtsh
Sat Jun 03, 2023 8:29 pm
Forum: Home-made microscope adaptations
Topic: American Optical Series 10 LED replacement for 1036A
Replies: 33
Views: 7417

Re: American Optical Series 10 LED replacement for 1036A

The phase condenser for the Series 50 has just four positions - one brightfield and three color-coded annuli. The largest annulus works for darkfield at 10x. The other two are for 10x and 40x phase objectives. The condenser attaches directly to the bottom of the stage and is a bit clumsy to work on...
by dtsh
Sat Jun 03, 2023 2:59 am
Forum: Pictures and Videos
Topic: Rat tapeworm egg in dog feces
Replies: 5
Views: 791

Re: Rat tapeworm egg in dog feces

I hope you find this article interesting. Does anybody in the forum also does coprological flotation for his pets? I also check fecal samples from time to time with the various animals that find themselves in my care. I use both passive floatation or a McMaster slide depending of what I'm trying to...
by dtsh
Sat Jun 03, 2023 2:55 am
Forum: Home-made microscope adaptations
Topic: American Optical Series 10 LED replacement for 1036A
Replies: 33
Views: 7417

Re: American Optical Series 10 LED replacement for 1036A

. . . I'd love to know what your opinion has been of that scope since I've been tempted to pickup phase for the Series 15...if I ever find such. The minus is the older or absent coatings lead to poor contrast, and the field size is smaller than I prefer. To my eye, the plan infinity phase objective...
by dtsh
Fri Jun 02, 2023 11:35 pm
Forum: Home-made microscope adaptations
Topic: American Optical Series 10 LED replacement for 1036A
Replies: 33
Views: 7417

Re: American Optical Series 10 LED replacement for 1036A

Following this with interest as I slowly get my AO 10s repaired and set up. I have looked at LED light sources over the years for a variety of applications, so I can suggest a type that may be of interest. The following COB LED has a very high CRI of 98, and notably does not exhibit the giant spike...
by dtsh
Sat May 27, 2023 8:44 pm
Forum: Microscopes and optics
Topic: Buying decision: Amscope T670Q, Nikon Eclipse Si, Motic BA310E
Replies: 16
Views: 2603

Re: Buying decision: Amscope T670Q, Nikon Eclipse Si, Motic BA310E

IMHO, the situation is not that bad, no need for such pessimism. I found that eBay's buyer protection is efficient and powerful. In contrast to the purchase of a used car, when buying a second-hand microscope, you discover the hidden lemon (if there was one) within a day or less, at no cost. Agreed...
by dtsh
Sat May 27, 2023 3:18 pm
Forum: Home-made microscope adaptations
Topic: American Optical Series 10 LED replacement for 1036A
Replies: 33
Views: 7417

Re: American Optical Series 10 LED replacement for 1036A

I've done the ceiling projection using a sheet of Matt photo paper,( my ceiling is 350 year old plaster lath)on a board. From my testing a single pinpoint source is optimal as a starting point. As it's possible to get a smaller pinpoint from LEDs up to 3w than an incandescent there's a good chance ...
by dtsh
Sat May 27, 2023 12:32 am
Forum: Microscopes and optics
Topic: What is the optimal combination of 5 objectives?
Replies: 10
Views: 3125

Re: What is the optimal combination of 5 objectives?

I don't use a 4x or 100x very often and I'm used to 4 objective noses (or even 3), so I typically omit them, but the setup on my 5 hole AO10 is 10x phase, 20x phase, 40x phase, 45x achro due to it's deeper working distance and a 100x. In general I think 10x, 20x, and 40x cover a lot of ground, espec...
by dtsh
Fri May 26, 2023 11:49 pm
Forum: Home-made microscope adaptations
Topic: American Optical Series 10 LED replacement for 1036A
Replies: 33
Views: 7417

Re: American Optical Series 10 LED replacement for 1036A

Use of existing parts motivated a lot of my parts choices based on what I had. Once I get to assembly, those of you with low tolerance for abuse of electronics components assembled by someone with a nearly complete lack of skill and understanding might want to look away. To provide the right output ...
by dtsh
Fri May 26, 2023 11:12 pm
Forum: Home-made microscope adaptations
Topic: American Optical Series 10 LED replacement for 1036A
Replies: 33
Views: 7417

Re: American Optical Series 10 LED replacement for 1036A

All of the systems that the series 10 was designed for in all magnifications with 176,176A and 184 eyepieces. BF at 40,60,100,150,200,400,450,500,600,675,750 and 1000X ; DF from 40 to 675X, Dark and Bright phase 100 to 1000X. B-Minus at 100X. Even fluorescence. High grade illumination systems are c...
by dtsh
Fri May 26, 2023 2:24 am
Forum: Home-made microscope adaptations
Topic: American Optical Series 10 LED replacement for 1036A
Replies: 33
Views: 7417

Re: American Optical Series 10 LED replacement for 1036A

Was actually asking for what regimen of performance tests. I thought " every thing I threw at it " meant that it had passed muster in terms of performance? So, I guess in the end you just threw money at it?. I've been asking for quite some time for specific tests and I've done what's been offered. ...
by dtsh
Fri May 26, 2023 12:21 am
Forum: Home-made microscope adaptations
Topic: American Optical Series 10 LED replacement for 1036A
Replies: 33
Views: 7417

Re: American Optical Series 10 LED replacement for 1036A

Just what did you thow at it? Money? Somewhere less than $50. It's hard to estimate as I had some parts already, but should be less than $50 with those added....should be. The price of the 3D printed parts should be minimal, but I have a 3D printer. Profanity? Not much actually! I only burned mysel...
by dtsh
Thu May 25, 2023 11:52 pm
Forum: Home-made microscope adaptations
Topic: American Optical Series 10 LED replacement for 1036A
Replies: 33
Views: 7417

American Optical Series 10 LED replacement for 1036A

First off, this is a work in progress. I've tested it and it's passed everything I've thrown at it, but I know it's not perfect and I sincerely hope others will help me continue to develop it. This started because I had a couple of AO10's with 1036A illuminators in which the wiring had failed long b...
by dtsh
Wed May 24, 2023 10:46 pm
Forum: Resources (online, books etc.)
Topic: How to tell Bacteria apart
Replies: 3
Views: 3373

Re: How to tell Bacteria apart

NachoBen wrote:
Wed May 24, 2023 6:13 pm
Excuse me, where is the link?
I was assuming the lack of a link meant the madness was real. :P
Plasmid wrote:
Wed Nov 11, 2020 3:11 am
... for those of you that want to identify Prokaryotic bacteria ( its pure madness) ... Enjoy
by dtsh
Wed May 24, 2023 3:05 am
Forum: Microscopes and optics
Topic: Which AO scope is easiest to convert to LED lighting?
Replies: 42
Views: 3959

Re: Which AO scope is easiest to convert to LED lighting?

I hadn't seen or noticed that control before, DTSH. To my eye, it's an elegant solution - especially if it allows ditching the external power supply and cables from early A.O. models. The science fiction Heinlein once wrote up a paragraph about things a man should know how to do, including changing...
by dtsh
Wed May 24, 2023 1:48 am
Forum: Microscopes and optics
Topic: Which AO scope is easiest to convert to LED lighting?
Replies: 42
Views: 3959

Re: Which AO scope is easiest to convert to LED lighting?

You neglected to mention loss of contrast due to scatter and lack of coherence. That's the led I'm talking about. How do you know that a particular diy led illumination system is as good, especially as a retrofit? I'm still looking for proof. What would you accept as proof? I am willing to do any s...
by dtsh
Tue May 23, 2023 7:06 pm
Forum: Microscopes and optics
Topic: Which AO scope is easiest to convert to LED lighting?
Replies: 42
Views: 3959

Re: Which AO scope is easiest to convert to LED lighting?

As stated earlier, it probably doesn't make sense to replace a perfectly good tungsten-halogen illuminator of 20 watts or more with a low CRI led, which may not even have the same lumen output. For DTSH's AO10 scopes, and for someone with his DIY skills, it seems (to me) to be a great choice. These...
by dtsh
Mon May 22, 2023 11:18 pm
Forum: Microscopes and optics
Topic: Which AO scope is easiest to convert to LED lighting?
Replies: 42
Views: 3959

Re: Which AO scope is easiest to convert to LED lighting?

What a skewed comparison. You appear to be using the incandescent light unfiltered? Why use that type of illumination without a filter? Because I was asked to image the field iris and not modify the result? I never claimed to be an expert, in fact I've been asking for recommendations of how to comp...
by dtsh
Mon May 22, 2023 9:52 pm
Forum: Microscopes and optics
Topic: Which AO scope is easiest to convert to LED lighting?
Replies: 42
Views: 3959

Re: Which AO scope is easiest to convert to LED lighting?

Since this is not my usual stand, but rather one I've been experimenting on, I decided to check it over and realign things as well as I can. I swapped out the phase condenser for a typical 1.25NA condenser (No Cat.# so I believe that would make it a Cat.1088). I have a Cat.1084 aspheric condenser to...
by dtsh
Mon May 22, 2023 7:50 pm
Forum: Microscopes and optics
Topic: Which AO scope is easiest to convert to LED lighting?
Replies: 42
Views: 3959

Re: Which AO scope is easiest to convert to LED lighting?

Thanks for taking that on. Test slide looks blue with LED. Yellow fringing is quite extreme for both, wouldn't know what's going on there if the incandescent filament is aligned properly. Is a diffuser between the light source and the iris? This LED is 5700K 90-CRI, so unsurprising it's more blue; ...
by dtsh
Mon May 22, 2023 6:04 pm
Forum: Microscopes and optics
Topic: Which AO scope is easiest to convert to LED lighting?
Replies: 42
Views: 3959

Re: Which AO scope is easiest to convert to LED lighting?

Just an image of the Kohler diaphragm would do with low power condenser. Maybe leave the wide yellow blue fringing rather than Photoshop it out. It's so much more than shine a bright light on it and all will be well. I tried to get the leaves as focused as possible. The only change was swapping out...
by dtsh
Mon May 22, 2023 5:16 am
Forum: Microscopes and optics
Topic: Which AO scope is easiest to convert to LED lighting?
Replies: 42
Views: 3959

Re: Which AO scope is easiest to convert to LED lighting?

Of course. However , the potential of engineered led microscope illuminators doesn't include diy led retrofits. The engineering of the AO illuminators is a defacto elerment within their construction that ensures a performance level that can be expected. There is no evidence that one can improve tha...