MicrobeHunter.com Microscopy Forum

Properly done GUF or oblique mask won't reduce resolution (because light angle does not change); they only increase contrast, which is unlike closing condenser diaphragm (which reduces resolution and increases contrast). In most transmission brightfield microscopy, we usually don't lack resolution;...

thanks ZZFFNN My main concern really is whether the oblique mask, or GUF - because it takes up so much of the underneath of the condenser, whether it reduces resolution or otherwise degrades the image as a result - a bit like closing the diaphrm reduces resolution in favour of contrast - does the GU...

wow, really nice detail - second and last two images are especially good.

lol edit; "last three images" would have been a better way to put it

thank you GaryB and 75RR. :) I would usually raise ISO up to from 800 to 3200 depending on the illumination - 3200 is pretty extreme case though, and for still objects i normally hover between 100 and 200 iso with shutter at around 1/20 but for moving subjects i try to use 1/125 or thereabouts. :) I...

My personal results from using the gradient filter are sometimes hit and miss, but I kinda like the look of this Paramecium shot - I had to raise the ISO right up for a faster shutter speed so its a bit noisy - seemed to turn out okay though with some noise-reduction in photoshop. Really glad to hav...

that is excellent work - think I might prefer the live diatom shots with the colour present, but they are all interesting subjects to photograph with some amazing images posted here.

thank you GaryB IanW SunshineLW and Vasselle - i've since managed to record a video of this paramecium type after finding one sitting still doing it's business for several minutes - good opportunity for video

Will post it to youtube, after it's nearly destroyed by compression of course

Radazz wrote:Wow! That is truly outstanding video!
Radazz

thank you Radazz. Really having fun with the phase objective

This was the other Paramecium type that I hoped I had identified correctly.. :) It looks really close to other shots ive googled, so I'm optimistic :P although pls feel free to supply alternative IDs if applicable Taken using the Gradient universal filter - I think it was with 40x leitz, NPL fluotar...

wow - amazing images

thanks guys - appreciate the feedback, and kind words

will be recording some more phase contrast stuff as it does seem to work okay - can't complain really

Ah yeah, I can get it so the rings are pretty concentric, but they are both different thickness, so that some of the 'bright' ring is visibly uncovered by the dark ring, around the edge, despite being aligned. I guess this is why the effect isn't as drastic or highly contrasted as some of the proper...

cool. the tip about embedding worked - thank you! Also, sorry Zzffnn just spotted your reply - many thanks for the input, much appreciated :) I added the specific parts involved, in case it rang any bells with someone - not an ideal match but it seems to 'do something' using bits I already had to ha...

BTW probably not useful info, but just to clarify the phase contrast setup - 3 unmatched parts, the BHTU of course - and a zeiss 25x planapo slim silver with dark green band [my keyboard is broken, so some numbers are an issue lol] it has fortysix - zero six - forty one - nine nine zero one, as ID. ...

Hi Aenima, To make the embed work you only include the last part of the URL: [ youtube ]zUzfRAezL8Q[ /youtube ] But it turns out it can be nice to include a link anyway just in case that doesn't work for some browsers. I will have to let someone else critique the phase contrast, but the larger cili...

zUzfRAezL8Q Hi guys - I have two questions about this vid - 1st is it Paramecium? ive been looking for paramecium for ages, and wasn't finding them in my moss and rain water, however I discovered a local park with a pond and had some good water samples from there - finally seeing some of the specie...

excellent work - love the 3rd image

GaryB wrote:You certainly have some cool goobers there, and the pictures are great. Nicely done!

Thanks GaryB

Stunning captures. Huge wow factor on that 2nd image.

Really nice shots. Is that DIC? thank u fibreoptix - i do wish it was DIC, but unfortunately no - it's the gradient universal filter mentioned elsewhere on the forum ( i think searching "GUF" will bring it up) :) It does seem to resemble the DIC effect in some ways, but i'm not always getting the s...

Taking a look at the gradient universal filter might be a good move towards getting a similar effect photographically - at least it is an easy and cheap solution.

viewtopic.php?f=6&t=5466&hilit=guf
Regards

Jay

Nice vid(s) - subscribed to your channel - thanks for sharing.

Hello Wes and welcome from myself in UK. :) I haven't been here long, and still a bit of a 'noob' but i wanted to post a welcome nonetheless. :) :mrgreen: I sympathise with the frustration in seeing problems in the optical quality - most of my objectives are Achromat design and give a lot of chromat...

Wow. Very interesting, and amazing images.

Beautiful pictures of a cute subject! Water bears are just the best little critters imo. Thank you vaselle :) - and GaryB, yes i also find them cute, and interesting at the same time. I can't get enough of the little guys - i have hours of video footage along with the still images. Must post someth...

Thanks guys. Hobbyst46 - they are all shot using the GUF (or UGF ) giving the oblique appearance - but also as i keep a piece of polar sheet inserted in the head, i can just add a second polar filter over the lamp for polarized effect, which is what i did here. The main issue is my GUF filter has se...

Tardigrade in oblique light (GUF) and polarized light. partpoltardyweb2.jpg also cheeky link to a flickr version :P :mrgreen: https://farm2.staticflickr.com/1782/28390690227_1c70b7419e_c.jpg Tardigrade/water bear pol by Jay Bird , on Flickr https://farm2.staticflickr.com/1821/29368921248_b41975e207_...

Wow, love these - especially the 3rd one down in green - has a nice outline and like a halo effect.

Hobbyst46 wrote:@Aenima
I entered the Flickr and observed your photos, and enjoyed them!

thank you very much Hobbyst46

Hopefully will add more photos soon.

these are fantastic - i really like the last couple with the colourful spikes on the diatoms.