MicrobeHunter.com Microscopy Forum

FYI: It is offered on ebay here: https://www.ebay.de/itm/295471899950?hash=item44cb7f652e:g:rt8AAOSwcnhjwoqX&amdata=enc%3AAQAHAAAAoMgBXeKwBNrlYQ1egPlH5ZKC5GdySpneTu2qm7zIKTj39WtHQNZv3QyqgC8sZrUjAGJIbvPo47kT%2FshY0MEGkDxId3jUOJLXzu3qu3muCTOEmr8xeON%2BZEmpw8f1Jym0gp1kLYdjuXkcv6p7zVUII6eA%2B9InQktwGSJA...

WWWW wrote: ↑

Sun Nov 27, 2022 8:32 am

I see you are using an inverted microscope, can't you examine samples without a coverglass ?

Yes, of coarse. But finally, I often try to observe the very granular details with high n.A. objectives. And this works best by flatening the ciliated under the cover glas pressure..

Naussla ornata is a fascinating, large ciliate and I enjoyed watching your video very much. It provides a good impression of the insides, with the large vacuoles and the oral apparatus.
I killed many of these fellows, when trying to fix them below the cover glas

Hi tlansing,
nice! Thanks for sharing

Just wonderful!

Congrats Scarodactyl to the IM35. It is a great scope! Obviously new kind of micro and lots of stuff to get into. Did you check already the HMC manual here: https://vdocuments.mx/hoffman-modulation-contrast-user-manual-supplied-by-ri-uk-and-ireland.html?page=7 Setting up the Köhler Illumination shou...

Very nice video, well done!

Really a interesting video, with a different perspective. Thanks for recommending and sharing the link!

Thank you for the comments WWWW and Tim. The Hyalotheca that I find in my local pond here in North Carolina have many pores in the mucoid layer surrounding the cells on the filamentous desmid. Do you see these in your samples? H. mucosa should have pores at the apical cell wall, but I failed to spot...

Dear foresters, during a short vacation I had the opportunity to visit a moor to take some water samples. Was quite happy to catch few desmidiaceae. Like to share some impressions with you. Site: Taufach - Fetzach - Moos (Germany, Württemberg Allgäu - September, 25th 2022) https://up.picr.de/4444412...

Hi Wes,
great series of photos!
The Planapo 25x is also my favorite Zeiss Objective, it makes a difference!

Fascinating, great video.

Nice! Thanks for sharing tlansing.

Hi Wes, maybe this test slide is quite interesting: 20220919_195311.JPG 20220919_194901.JPG 20220919_194949.JPG 20220919_195021.JPG The plate is called "K5" On the glas slide is a round, galvanized silver mirror with (engraved) parallel "zick-zack" lines. Such lines are also used on the Abbe's Test ...

Hi Wes, May I ask you where you got the white grid on a dark background that you used for comparing objective performance? There was a box with Zeiss test slides included when I bought one of my used, old Zeiss Standard microscopes. So was very lucky to get these as free add-on, included in the pack...

Great thread about the pedal switch. Good to see what is your experience. There are some criteria that make a perfect pedal switch. I am using a pedal switch that is using a jack plug, conneting directly into the camera. They are nice, but I found that the force to activate the pedal switch was too ...

Can also highly recomment: Understanding the Light Microscope by Peter Evennett 60_jgZtyR6U 1. Conjugate Planes in the microscope (0 – 21:00 min) The optical system of a microscope embodies two series of planes which are conjugate – optically linked, as images one of another. These are the ‘Field’ s...

The "standard objective" is designed to compensate for the spherical aberration of a 0.17mm coverslip Therefore, the objective lenses used in inverted microscopes usually have a "correction ring" Often the inverted objectives are also called "long (working) distance ("LD") objectives & the objectiv...

Congrat's, really very nice images.
It is always interesting to see details of the setup and equipment. So it you like please feel free to share

Hi Christian,
FYI: The modell is a Zeiss Standard WL.

Hi Luis, really impressive! Thanks for sharing.

Hi Javier,

Given the poor quality of my microscope

I like the video, rather good quality and result!

Nice catch Javier! Thanks for sharing.

Thank you tlansing. This rotifer is really majestic.

Well, finally you may try very unpolar solvents, like xylene or petrol ether.
However maybe the lense coating or the glas itself has kind of corrosion... so no solvent at all will help.

Hi Javier,

I missed the size factor

Exacetly, truncatella should be really a big fellow.

Also enjoyed watching, thanks Francisco. The later sequence of the video was done with with phase contrast?

Nice video, thanks for sharing.

What a wonderful video! Thanks Francisco for sharing.

Lots of interesting details. Thanks for sharing!