MicrobeHunter.com Microscopy Forum

I have two options: 1. Drive to 80 km to the nearest university library and ask visitor password valid for the rest of the day. 2. Use Sci-hub You may now guess which one I use. I say this as a molecular biologist who frequently reads papers. However, not frequently publishing because my research is...

Some stuff from your culture medium I think. It is my experience that samples taken directly from culture medium seldom give acceptable results. 1. From culture plates: pick a part of a colony and suspend into a drop of water on slide. 2. Liquid cultures: centrifuge a sample of the culture and disca...

The methods mentioned above (antibodies, GFP) may be very expensive to use. It may also take months or even years to get a particular application to work even in a professional lab. There are also some organelle-specific dyes which are rather easy to use. They may be rather expensive too and I am no...

It is extremely simple. Just a bridge rectifier with a smoothening circuit. You may find almost an identical schematic e.g. here https://www.powerversity.com/what-is-a-bridge-rectifier-circuit-diagram-of-a-bridge-rectifier/ All the components are from my spare part boxes and the actual values of the...

see if the magic smoke arises or not

If this is like Lomo transformers try to keep the smoke inside. They a powered by smoke and if the smoke escapes they do not function ay more

I have no experience with Jena microscopes. However, in Russian Lomo microscopes (I have owned several of them) they sometimes used mains-type plugs with low voltage systems. These mains-plugs fitted into transformers, however could be accidentally inserted into mains as well. This might be fatally ...

A couple of pictures from my family album. My late father was a chemist in a rubber factory. In the first picture he is doing some microscope work in a lab 1934. The second picture was taken a few years later 1940 during the Finland-Russia Winter war after Soviet bombers attacked the factory. Most o...

Great idea!

I just stopped using immersion oil because I have to spare all the possible flammable solvents. Just in case I need them for making Molotov cocktails. I live just a few km from Russian border. We are ready in case you did your very last fatal mistake, mr Putin

After reading this article I bought the Risingcam. I am very happy.

I use heptane.

My Leitz Orthoplan manual suggests using xylene, which is not recommended nowadays for safety reasons. It is rather nasty-smelling anyway.

After lost my university career I established a private research lab. I had no money for equipment for making deionised water and making distilled water is both too time- and energy-consuming. So I have simply bought water form a local discount store meant for car batteries. I think it is deionised ...

I have the same experience. I have old short barrel objectives in my Olympus FH and they work just fine. Extensive objective comparison and aiming at perfectionism with the image quality is fine if that's the part of the hobby. Otherwise the most important is that you get the information from the im...

I have submerged the whole iris system (without optics of course) into mineral spirit for some time. The grease dissolves and after that you just let the iris to dry. You may use a more volatile solvent e.g. hexane (or use hexane as a second solvent) to speed up the drying of the inner parts. Doing ...

Chromosomes are difficult to find without using special techniques. The best way to start is to use onion root cells. You may google plenty of procedures. According to my experience the most important thing is to use proper material. You grow roots and only use the very fresh tips. I think any gener...

I don't know about microscopes for sure. But my grey Ferguson tractor (1949) has also 6V system. Maybe it was more common in ancient times and has some historical background.

Happy New Year!

your collimation of the beam is probably not optimal.

Sure it is not optimal. However, now it is done so let it shine...

I was wondering how the progress is going with the lamp LED.

I placed my recent progress here:

viewtopic.php?f=24&t=9420&p=115717#p115717

This is the version 2.0 of my fluorescence LED illuminator for Orthoplan. I am not a fan of 3D printing and such things or even know anything about it. Besides I like to do something with my hands. I bought leds of 30W 450nm and 470 nm. The first experiments are very promising…. Changing the leds is...

There are several Orthoplans in US in Ebay at the moment. Plenty of objectives too. Extra turrets are not more easy to find in Europe I think. They show up in Ebay from time to time. I don’t find the extra wide field so useful one might think. Although the view is impressive, it is somewhat tiring t...

Sounds like it is working fine. Keep on eye on the current, I was blowing 10W into my 3W led without noticing... If I ever happen to blow up the led, I change a spare led :) And here I just extended an old Olympus transformer for use of led as I think the original transformer is so nice and I want ...

Long before the existence of the mighty Ebay I used to make some electronic devices myself. Not that I really understood anything about electronics, however construction is possible if you follow the circuit diagram. Here you may find the circuit I used in many applications for voltage regulation: h...

Thanks for the presentation! As I said I have used variable voltage. It works for me, however doesn't seem to work for others :) As I have systems ready, I don't thing I am going to start building everything from the start again at least in the near future. However, I will keep all this information ...

You may find more information here: https://www.microbehunter.com/microscopy-forum/viewtopic.php?f=11&t=13170&p=113413#p113413 I have used variable voltage regulation for dimming leds until I learned here it doesn't work. The current project is to make a multi-purpose illuminator base/lamp house int...

For a creative and multi-talented person it might be difficult if not impossible. So let me put it this way: Why should it be ordered? All kinds of stuff around brings inspiration.

Not so impressive... The led system for the transmitted light is working well. The system for epifluorescence doesn't as it would need more light intensity. I am currently working on it to improve the system. Have many other projects so the progress is slow. However, I think should get something rea...

Do you have a frosted glass diffuser under the condenser? It is needed with the basic illuminator. I have the original Lomo köhler illuminator. It is not bad, however I would spend my money for some other microscope of higher quality.

Sure, it will just add a little length. Does it work? What happens to the image quality? I might try to use Olympus Neo objectives in RMS turret as I have both a couple of Neo objectives and an epi-illuminator for those objectives. However, I have Olympus FH stand with RMS turret. So this would be ...

Your system seems to work very well. It really makes me think which would be the best option. The problem with leds is that the wavelength does not match quite optimally to the filter cube. And there is more or less limited selection of leds with different wavelengths. Making a quick test with a che...

I ended up going with a xenon bulb from a car

How well does it work e.g. with a GFP/FITC filter? How is the intensity?