MicrobeHunter.com Microscopy Forum

I think this might be a representative of Peranema (or the closely related genus that Bruce mentioned):



Definitely an heterotrophic euglenoid. Hard to tell from the B&W video but there were no chloroplasts; plenty of what appear to be starch grains though.

...There's an article published last year titled "Differential interference contrast microscopy with adjustable plastic Sanderson prisms" that may reveal a plausible path for DYI DIC. It's available here (for a fee): https://www.osapublishing.org/ao/abstra ... 59-11-3404 That paper was mentioned in...

BM = bright medium, aka negative phase contrast.

Thanks, that makes sense.

LOMO objectives with a B.И. label (and white ring) are Water Immersion objectives. The markings of that 10x/0.25 objective that you show in that last image say B.M. (not B.И.) and have the white ring, so I wonder if that's a water immersion objective and not a dry objective like the 40x that you sho...

Pretty good results for a DIY anulus. Regarding your problem setting up Köhler illumination after removing the frosted glass, you can either use the condenser height adjustment knob to focus the field diaphragm within the field of view of a focused test subject (with a closed condenser aperture diap...

I've installed the software from Hayear in Windows, both the toupview-like interface and the newer 's-eye' software from their website but I like the toupview-like interface better. In Linux I've used webcamoid (liked it, no frills video/photo capture). The level of control over camera parameters th...

Success! After fiddling with the Lumenera driver code included in the SDK for Linux I was able to get the drivers loaded and the camera recognized by the kernel. Fortunately both, the USB and FPGA firmware, are all loaded by the drivers. The only obstacle to get this going in Windows with the Lumene...

Did a bit more digging around. The PAXCAM hardware is really a full Lumenera's LU-200C camera board (full SDK is available for it, neat). The LU-200C board uses a Cypress EZ-USB FX2 microcontroller as the USB bridge, paired up with some I2C EEPROM chip that likely holds a customized firmware version...

Thanks Bruce. Amended my post accordingly.

A couple of videos of sessilid peritrich ciliates. I think these organisms belong to the Epistylididae family, probably to Rhabdostyla genus (thanks Bruce!). The stalk appears to be non-contractile, but the body definitely is. Seems to have only one row of cilia in the peristome. I can't really see ...

I bought a similar one for my son from Amazon a while back. When we tried it, the image was way off, on the borderline of an instant headache. I opened the head and found out that one of the prisms was loose and out of its socket. Not a surprise given its cost. I think I had to hot glue the prism ba...

I'm interested in finding LOMO's 20x/0.70 and 40x/0.85 objectives (infinity and 0.17 coverslip corrected); the green and blue ring objectives in the picture below.

Thanks for the help MichaelG. I've seen the specs page from photonics.com before, but not that ebay auction. You are right, that auction is a no-go to get a driver. Shame, its the exact same model. Seems the camera has quite a decent resolution 1600x1200 for an oldie. Would be nice to get the TWAIN ...

MichaelG. wrote: ↑

Mon Mar 22, 2021 9:10 pm

Doesn’t look very encouraging:
https://www.paxit.com/resources/technical-support/

Nope. Been there, chatted with a rep, sales rep never got back to me. That's why I posted here, but everything points towards the camera going the paper weight way.

I am fairly sure I have seen pictures of objective lenses with laser holes burned in them One can only hope that whoever was messing with those lenses and a laser knew what he was doing! I'd imagine that you'd need some serious laser to do so, though apparently an 8W laser would be able to let you ...

A PAXCAM (model PX-CM) was included as part of some hardware I recently acquired secondhand. As far as I know its an old camera model with a USB 2.0 interface and a sensor of 1M pixel or less, yet its apparently sensible enough to be used in fluorescent microscopy applications, and the reason why I'...

In my naivete I was influenced by an ad for an advanced microscope system that apparently uses laser light to get dramatically better resolution. That's why I tried it. Laser light is indeed used in microscopy to dramatically increase resolution by techniques such as confocal laser microscopy, supe...

I also have this LOMO microscope, known as Mikmed-2 or Laboroscope in the US. It is a nice microscope. You can find some info in the old LOMO US webpage stored in the web archive (wayback link here ). I was lucky enough to find a new and complete phase contrast kit for it, C-PHM or KF-4M, here in th...

Adding to what others have said, you'd also would need to keep in mind that the aperture of the system might be limited, likely by the maximum NA of the condenser. I would say that this is typically the case of inverted microscopes used mainly for tissue culture, with a fixed condenser of an NA some...

... did you view the sample directly on the dish or was it prepared onto a slide,?

All was imaged directly from the petri dish, no slide preparation.

A few videos of the same amoeba under different contrast methods. The amoeba is about 35 micrometers in diameter, slow moving without pseudopods, and has several inclusions (glycogen bodies and/or crystals?). I'd say its from class Discosea, but can't venture further with an id. It was found in a pl...

Is this the one from American Science and Surplus? If so, just email them and ask them to measure what you need from it for you. Note that the stand is also missing parts from the illuminator: the field diaphragm and collimating/focusing lenses. Take a look at this , its a variant equipped with epi-...

Hobbyst46, your thoughts on this are much appreciated. 1. Possibly, the lamp position is adjustable, so the distance between the filament and mirror is adjustable. This might be needed to create Kohler illumination (if the microscope is indeed designed for such). Also, if there is an aperture iris b...

Hi all, I'm restoring an inverted LOMO Metam (RV-21 or IQ) microscope, but I'm having trouble aligning the mirrors that reflect the light from the illuminator (on the left in the following picture) into the objective. The microscope is infinity-corrected, equipped with LOMO achromatic epi-objectives...

Its a coiled gastropod (aka. snail). The shell, cephallic tentacles, eyes, and proboscis are clearly visible. The foot can also be seen, as well as a small piece of shell in the foot (the operculum). Here's an image from the body plan (grab from the internet, but it was originally in Invertebrates, ...

Look like ostracods to me.

Its a segmented worm (annelid).Take a look at the thread here.

I have a similar camera (from Hayear too). The common theme in all these lower tier cameras is that you can only adjust exposure, white balance, etc, when its connected directly to an HDMI monitor. Also, photo/video capture at the full resolution of the sensor (and highest possible frame rate) is on...

Indeed several of these annelids appear in the image on your post, perrywespa. Here's the link to an impressive video of one Aeolosoma specimen, posted a while ago by another forum member: viewtopic.php?f=6&t=7113&p=62921&hilit=Aeolosoma#p62921

Those are segmented worms (polychaete annelids) from the Aeolosomatidae family that live in freshwater, so you were close when you were thinking annelids. Look at these one: https://visuzoologia.tumblr.com/post/49272934159/aeolosoma-hemprichi-by-proyecto-agua-water A bit more from the wikipaedia: ht...