MicrobeHunter.com Microscopy Forum

Well zzffnn and gekko, it is a wet mount, also I added more water to the slide and nothing ( so not a crystal of m.b.). It appears to be stucked to the slide ( I fixed the cells with a lighter). The thing is the slide was pretty normal ( muscles cells, epithelial cells) and all of a sudden this. I v...

Hi there I just found this while centrifuging a blood sample from a beef. It is stained with methylene blue and is around 35 microns. Does anyone have an idea of what it could be?

Thank you 75RR for the link and thank you Peter for telling me the concentration and type of solution.

Best regards my friends.

Also if anyone can explain me what are the advantages and disadvantages of working with an aqueous solution or alcoholic solution please.

Hi guys, I recently bought Safranin as a powder, and I would like some advice on how to make the solution (if aqueous or alcoholic) what proportions should it have and what concentration of the dye. I am looking to do Gram stain but also general nuclear stains and cartilage stains. Please if you can...

Very good and sharp image+video, also thank you for describing how you enhanced them. Keep up the good work.

Hi John B, beautiful desk you have there and very well organized, I especially like that you are meticulous with your work. Thank you for sharing your protocol and photographs. Best of luck.

P.S. by the way from where did you get formaldehyde in England ?

Thank you and keep up the good work.

Hi charlie g thank you very much, indeed I learned a valuable lesson (not to say: o, I will identify this organ after the staining :D). Thank you for sharing that image, it was kinda the same that I saw and decided that the chicken will serve a even higher purpose for science (also cooking). Nonethe...

I believe that as charlie g named it so poetic, Viscera unknown is a lymph node. I searched thorough a lot of histology book and all indicate this. It was hard to distinguish because my section was not sectioned at the proper size of a 6-10 μ, instead with my hand made microtome I think it is around...

B zone + cells that were removed from the tissue (seem like epithelial cells),800x magnification of epithelial cells, 400x magnification of B zone

A zone ; Magnifications in order of images:100x,200x,400x,400x with 2.5xzoom,800x, 800x with 2.5xzoom

Additional photos.

I've been having a go at making my own histology slide, but I encountered an interesting problem. First, the organ tissue was taken from a frozen whole chicken bought from the supermarket, it still had both lungs,both kidneys and what I tought to be a blood vessel from one kidney, I will post pictur...

Fantastic pictures, book quality there I'm glad to see you're still excited by your work. Keep up the good work.

p.s. the methylene blue pictures seems to have a little metachromatic effect on it, do you know a method of emphasizing the effect?

Raul.

Thank you for your reply John B, it seems that 1% at 2 min does the job well compared to the rest. Thank you for your advice on Safranin and Orange G it may come in handy at later times .

Beautiful

Well it kinda looks to me like it, having dendrites, but the astrocytes are only present in the brain and spinal cord, it dependes from where this sample was taken.

As the title says I want to know what the suitable concentration of Eosin would be for most histological applications. I checked on the internet and some proposed 1% other 0.5% and even 0.2% (of course the staining time differed with each concentration). I would wish to know from someone that had go...

Incredible, thank you very much!

Thank you 75RR, I did come across his name about some months ago but I totally forgot about him. Thanks for refreshing my memory.

Thank you Seb28, the last link had some good information on quantities. I will experiment and if those look fine I will post some pictures:).

gekko wrote:Nice work.

Thank you very much .

I think over there.

What was it stained with? Also can you take a picture of an astrocyte (I think I can see one about 2/3 to the right-down corner). It is a very nice sample of tissue, a beautiful piece and a lot of work and trails. Please post more .

Thank you for showing this.

Hi guys I want to make the solution using methylene blue and eosin. I am interested in the proportions to use and also other aspects like buffers or oxidants for m.b.. I know that I can buy a cheap Giemsa stain, but I want to explore the science behind it.

Thank you.

Another excellent picture - very nice indeed, I'd be very proud of my efforts if I'd created these images and the slide/s from which they are taken. Well done. :) Thank you very much, also you are a bit to blame John B :) that I bought H and E (giving me links to some sites, and more other advice)....

Thank you very much for your kind words,mrsonchus and JimT, the magnification was 400x microscope and 2.5x from camera. I have another sample with a bigger camera zoom 3x( this time on purposely overstained with Mayer's Haematoxylin) to have a better visibility of the nuclei. Although the striations...

My first histological slide using H & E stain of pig skeletal muscle. It is mounted in glycerine-jelly (R.I.=1.47)
Hope you enjoy it.

Hi Naphthalene, congratulation for the samples, they are indeed with a lot of information and also they present a sweet for the eyes. I do have a question for you, did you post-process the images with a software (like photoshop or something similar) ?

Wow mrsonchus you have a lot to show my friend. Please keep your microscopy passion so that we can enjoy the show . Very nice images, I like the clarity in them, the information that you show and the simple protocols that work very well. Keep up the good work.

Kind regards, Raul.