MicrobeHunter.com Microscopy Forum

Connecting full frame DSLR to a microscope is easy, as before the digital age 35mm was the smallest size film media which would be use for photomicrography. Most older photo tubes and ocular so are designed for 35mm film or 4x5" sheet film. The resolution is another matter. The higher the resolution...

75RR wrote:

Zeiss used to make a special stand for WL which would allow mounting f the camera without physically connecting it to the scope.

Do you have an image or catalogue number for that?

47-62-50

75RR wrote:

Zeiss used to make a special stand for WL which would allow mounting f the camera without physically connecting it to the scope.

Do you have an image or catalogue number for that?

Yes, I can look at the part number on the base.

I notice vibrations at high magnifications if I don't use mirror lockup. My photo setup, which is Canon DSLR connected to Universal through upper port is quite steady. Enough so that I do not need to use air table (I have one). I did attach furniture felt feet to the base of the Universal, mainly so...

This is my setup. I'm fairly satisfied with results.

Well, the actual amount I paid for this is zero. I inherited some prototypes when I took over the department, and since some of the prototypes went few years back and no one knew what they were, they were discarded. I took several interesting contraptions for myself, as they were heading for the tra...

When I just got this device, I experimented quite a bit with it, as my idea was to obtain a second one, disassemble it and insert the second FO cable on the other side after drilling and shampfering a hole. The project never came to fruition since I was just too busy. The way the sample is illuminat...

Stopped down by objective iris.
Interestingly enough, I would not qualify this illuminator as transmitted light. It is more similar in its principe to incident light, as the sample is illuminated from the side and is positioned against opaque background.

I did not mill the fixture myself, but it is of a very simple design. I can probably manufacture something very similar using a tabletop CNC milling machine. Basically it is an aluminum block with a pocket milled into it to slightly loosely fit standard slide. There is a gasket (not visible on the p...

Disassembling semi-flexible drive shaft for Zeiss inverted microscope stage is a challenge due to plastic joint inside. Lubricating with high lubricity thin oil did not help, neither did a bath in WD40 ( I took the shaft off the stage of course, it is held by two screws). Applying neutral pH petrole...

As far as resolution goes, 5MP is plenty, but as you said, the driver could be a problem, no service, and at $500 you can buy modern generic camera based on Sony CCD, which will probably get you better imagery anyways. In addition to MP, these old chips don't have allot of dynamic range, which has b...

Here is DF fixture with fiberoptic light guide which provides illumination parallel to the plane of the slide.
Obviously I turned it upside down, as this is on the stage of inverted scope

zzffnn wrote:Oktagon,

May I see a photo of your darkfield setup using fiber optic cable coupled to slide? Thank you.

Yes of course. I took a picture today, I just need to reduce it so that the forum will accept it.

I might be interested in the Universal. Please PM me the price.

Not to many posts this forum...

I have come to a possession of Zeiss ICM-405/IM-35 stand. I am currently trying to get it operational to the level of my Universal. The stand came with no objectives, but with both illumination systems and 0.6 OA condenser. I need to add specimen holder to the stage, phase condenser and objectives. ...

As a LOMO user myself, I would like to see photos of your LOMO gear sometime if you still have it. :mrgreen: CE Sure, no problems. I'll take pictures tonight. So here is late 70's Biolam with AY-12 binocular head. Although right now it is wearing a mirror, I do have OI-35 illuminator. Two of the ob...

Also, what is the difference between ICM 405 and IM 35 equipped with 4x5/Polaroid focusing screen in the front? They appear to be the exact same scope...

As far as LWD objectives go, I use standard planapo objectives at work on the inverted scopes, except for the ones I use in tissue culture room, including OI objectives. Is it different for these older 160mm tube optics?

The transmitted condenser is a low 6.3 NA condenser. Here is a fully loaded Zeiss ICM 405 with transmitted 6.3 NA DIC mounted circled in Red and the BF/DF or Fluoro Epi condenser circled in Blue. You will also need LWD objectives. ICM 405 Left.jpg How complete is the IM/ICM you are getting? Thank y...

I recall seing what appeared to be standard substage phase condenser mounted on inverted scope ones. I suppose you can take the top lens off and bring the condenser as close as possible to the object.
Well, looks like i will have another project going.....

The upper transmitted condenser. Phase or DIC, depending on the condition and price. I might end up inheriting IM-35 (or ICM 405, I'm not even sure, as I only saw it for a second) body, so I will start putting together a working scope. I' have never dealt with pre-Axiovert inverted scopes, such as I...

I'm not even sure I'm following forums rules by opening a thread in this branch. Anyone has any ideas on the source for such condenser?

Crater Eddie wrote:As a LOMO user myself, I would like to see photos of your LOMO gear sometime if you still have it.
CE

Sure, no problems. I'll take pictures tonight.

There is a company that made fiber optic attachment to slide for darkfield and they claim usable darkfield up to 100x objective. What is the highest power objective that you tried with your FO/slide in darkfield? And how was the image quality? At 10-30x objective, as long as working distance is eno...

BTW, I received couple of PMs, and must apologize for not replying. Since I just registered, I can't send private messages yet.

Yes, in fact I do have OI-1 dark field condenser for LOMO! I think you meant OI-13 (oil DF) condenser? Do you have an iris objective and/or funnel stops? I am only aware of a funnel stop for LOMO 90/1.25, 60/0.7-1.0 oil riris objective, 50/0.7-1.0 and 30/0.6-0.9 water iris objective. Yes. Exactly O...

Yes, in fact I do have OI-1 dark field condenser for LOMO!

Thank you! It is a very nice scope. After I spent some time cleaning the internals and lubricating focusing works, it is silky smooth and very stable for photomicrography. I ended up modifying the photo tube in order to make it confocal with Canon 1Dx DSLR I have attached to it. The photo tube I hav...

I have been lurking on the forum for a while, but finally decided to register. I've been hobby microscopist pretty much since my parents bought me microscope set for my 12th birthday back in the 80s. Curtrently I use scopes for work as well as for play at home. I have decently equipped home lab and ...