MicrobeHunter.com Microscopy Forum

Great image.

Beautiful.

Thank you Francisco.
The condenser is Dic?
Do you you recognize what those two parts are on the left side in first photo?

Does someone know is this a complete dic system for Polyvar microscope. ?

Beautiful.
What means "2.5pe"?Photo eyepiece?
I would also like to know what is your microscope setup.

Hi William. A few years ago, when I didn't have a complete DIC system,I tried different combinations to achieve DIC. I get a good result with INKO prism epiplan 16/0.35.The prism was placed under the condenser and and worked with the PZO DIC head. Here is the link: https://www.photomacrography.net/f...

Look's like "Chaetogaster sp."

It looks like "Merismopedia sp." on the first picture.
on second picture probably Coleochaete sp.

Check out this post.
http://www.microbehunter.com/forum/phot ... ediastrum/

http://www.tandfonline.com/doi/abs/10.3109/10520296709115015?journalCode=ibih19 Stem, leaf, and bud tissue of sweet potato, tomato, and pepper were embedded in paraffin, sectioned, mounted, and stained with 0.01, 0.1 and 1% aqueous and 0.1% alcoholic solutions of acridine orange. Temporary and durab...

You can get much better effect staining by clearing the sample before staining in lactic acid or clove oil to get them more transparent.

Nice.

Thank you all.

Nice JimT.Did you clear it before staining?

Great spores image.
But in my opinion they are little overexposure or has to low contrast.

Very nice Gekko.

Very nice.Did you make and stain the slide?

hkv wrote:Outstanding! Fluorescence?

This is not fluorescence,I invert bright field image in Photoshop,and play with the colors.

Thank you all for your kind comments. I begin by placing the specimen in KOH/NaOH solution, heat up to ∼90–100 ◦C,5-10min, preferably in a water bath,then washing in pure water. The most difficult procedure which is a placement and positioning the insect on the slide. In the center of a microscope s...

Drosophila melanogaster cleared in KOH,whole mount in glicerine.

Very nice images.

Beautiful.

Very nice.

Beautiful.

Very nice.

Very nice Hydrachnidia

Beautiful image.

Thank you mrsonchus and JimT for your comments.
I have some feeling that there is little over staining,

easyzoom
Thank you for your advise.

How to add the easy zoom link?

This is an unidentified plant,leaf stem transvers section.
Fixation-FAA
Emmbeding in PEG-1500
Hand cut section under stereo microscope.
Stained with Crystal violet,diffrentation with HCL-ethanol,stained with Safranin O
70% Glicerine mount
[easyzoom]https://easyzoom.com/image/94291/[/easyzoom]