MicrobeHunter.com Microscopy Forum

Collapsible golf ball retriever adapted to take a small plastic bottle on the golf ball end. Here I must give a plug to Tony Pattinson's excellent books "The Freshwater Microscopist" including parts 2, 3, and 4, all available from Blurb Books.
Tom W.

Very nicely shot video, though I'm afraid I can't help confirm the ID. Thanks for sharing.
Tom W.

Thanks very much for the link 75RR. Looks like a fascinating read.
Tom W.

Hi Kirby Looks like a very high quality vacuum table - lucky you! Unfortunately I live a little bit out in the sticks, well... that's not really unfortunate at all, but it does mean the nearest colleges, universities, research establishments etc. are a long way away, so auctions such as these are no...

A worthy project well worth the invested sum. I still don't own a 3D printer but seeing what you guys are doing with them has piqued my interest. I assume you have to create a program to print something like this? I'm not very computer savvy so that's probably my main hesitation. Are those all your...

Ah Timemaster... I know exactly what you mean. But you have to be careful of those "short term options" that have a way of becoming "forever options"!
Tom W.

Kirby Thanks for the kind words. For printing I used a standard Creality Ender 3 printer which is relatively small and cheap but still has a good build volume. This project doesn't exactly stretch the printer to its limits on size but it is getting close. I used a PLA filament that I buy for $35.00 ...

I have been intending to do something about slide storage for some time and was finally inspired by Element 56's post at http://www.microbehunter.com/microscopy-forum/viewtopic.php?f=11&t=5953#p84057 . For someone who does 3D printing this may be an acceptable option - not as elegant as Element 56's...

Wow! Beautifully done. A lucky chance wonderfully realized.
Tom W.

Thanks to Carl for this, and to 75RR for drawing our attention to a very useful contribution.
Tom W.

Beautiful! A tribute to your skill and patience. Thanks for sharing.
Tom W.

Superb! Thanks for sharing.
Tom W

Very nice video indeed. Nascent cell membranes??
Tom W.

Hi ImperatorRex The Mikrokosmos articles appear to be very good, but they are a difficult job for a non-German speaker to translate, even with the help of Google or Microsoft translation services. I have attempts at translating them tucked away somewhere but the translations are not very lucid. But ...

Thanks for the link microb. Very useful background information.
Tom W.

Excellent job Bram. Really nicely done.
Tom W.

Excellent, excellent, excellent! Wonderful images. And you are absolutely correct - there's no need to be envious of super resolution SEM images when we can be envious of mrsonchus' LM images :mrgreen: Tom W P.S. The dandelions in my garden have no need to worry... yet, since they haven't yet appear...

Very interesting series of posts. Thanks for starting it off, MicroBob, with your example of a variation on an old method that appears to give excellent results.
Tom W.

Wow BramHuntingNematodes that is one shiny, shiny tabletop! I like it very much.
Tom W.

Thank you. Great images. In the latter three images, which I assume to be with acridine orange, there appears to be a superb differentiation of tissues, if in fact the various colours actually represent different tissue types. Is that actually the case? Tom W. Yes ,acridine staining make differenti...

Great images. In the latter three images, which I assume to be with acridine orange, there appears to be a superb differentiation of tissues, if in fact the various colours actually represent different tissue types. Is that actually the case?
Tom W.

Seb28 wrote: ↑

Sun Apr 05, 2020 8:29 pm

Hi Seb
Can you tell us what PEG molecular weight you are using for the hand sectioned samples?

I'm using PEG1500.

Thanks Seb. Sorry for wasting your time! I already edited my post to tell myself it was 1500, as stated in your original post. Duh!!
Tom W.

Hi Seb
Can you tell us what PEG molecular weight you are using for the hand sectioned samples?
Regards
Tom W.

Edit: OK - brain fade - I just re-read the post - dilute 1500 is the answer! (Or is it 42?)

Very nice result indeed Seb. From your description it sounds like you cut the section by hand. If so you did an excellent job.
Tom W.

Excellent, informative, visually compelling, keep 'em coming!
Tom W.

+1 re: MichaelG's post.

I have had some problems with some of the articles Mr. Smith has published on Micscape in the past. I think the current article is the most problematic to date.
Tom W

Beautifully done sinabro. Thanks for sharing.
Tom W.

Hi Antoni
+1 re: daruosha's post, and don't worry about the delay - we appreciate the time and trouble you have taken to put this together.
Tom W

Think not what you can't see that the SEM can, but what you can see that you couldn't before you had a light microscope.
Tom W.

Thank you AntoniScott. Very interesting video. Really good to see these things done rather than just reading a description which, no matter how detailed, can never capture the nuances. Looking forward to part 2!
Tom W.