MicrobeHunter.com Microscopy Forum

John, I stand to be corrected, but I am sure that 'built-in iris diaphragm' is not the same thing as 'correction collar' ... I think you will find that the diaphragm serves exactly the same purpose as the one in a camera lens. I have never seen a low power objective that needed a correction collar. ...

Thank you ... that clarifies nicely. The main reason that I asked was because I could see no reference to an eyepiece. >> It is now clear that you did not use one ... I also did not use the eyepiece in the home made camera mount tube. ... which makes the performance of the objective all the more imp...

That looks very promising indeed !!

Some technical details of the set-up would be appreciated.

MichaelG.

I'm pretty sure that you are right, Pat
... see my note [Wednesday] on your other thread.
viewtopic.php?f=6&t=5450

It certainly got me thinking though !!

MichaelG.

Thumbnail version

And there is also some light on the question left from the previous thread . It is not evident while the rotifer is contracted, but at 2:20 the one shows a round bubble at the cloaca, which then abruptly contracts and disappears. Thanks for that observation ... Your analysis makes good sense. Micha...

Astonishing
... and I think it's even more impressive played at high speed; which gives a great sense of the Action.

Thanks
MichaelG.

Oops ... I had missed that one
Thank you, BillT

MichaelG.

Lovely work, Pat ... The dark-field works a treat.

MichaelG.

Jimalin wrote:Michael G.
I am interested in talking about that Olympus adapter. Please send me a message to cooleysan@me.com.
Thanks,
Avery

eMail [with photos] sent a few minutes ago, Avery

MichaelG.

Avery, Please let me be the first to welcome you to the forum ... I'm quite new here, myself. I have a grey 'Photomicro Adapter L' available, if you can't find a beige one. I was intending to put it on ebay, but, if you are interested, please send me a message, and we can discuss. Best Wishes Michae...

That's a good looking instrument, which should serve you very well.

MichaelG.

Revised version: As I hope I made clear; [in common with most of mankind] I know very little about the rotifers . However: If [as seems very likely] the object is not a testis, then it does surprise me that it is so clearly visible in just two of the individuals. Is this just an optical anomaly? ......

If I am understanding this correctly: You appear to have some Male specimens in your herd These rotifers are bdelloids. They don't have any males at any point – < etc. > . I defer to your wisdom ... and would do so unconditionally if I had not seen the claim in Richard Dawkins' book "The Ancestor's...

The more I learn, the more I realise how much more there is ... http://www.rotifera.hausdernatur.at/Species/Index/84 I've linked to that particular page because the Observation images [click the image to view full-screen] include a good one of the Male [ANSP 948]. ... fascinating creatures. MichaelG.

I have just re-found this page, which includes a useful note about the Visual and Photo variants of the K7x eyepiece.
http://www.microscopy-uk.org.uk/mag/art ... photo.html

Slightly off the main topic of this thread, but I thought it worth linking.

MichaelG.

Hey -- I've got a question concerning rotifer physiology. I noticed a couple of the rotifers in the "herd" photo have a round thingy of some sort in the second or third posterior segment. Does anyone know what that is? Thanks! . Pat, What I know about rotifers could be writ large on a postage stamp...

I thought it was odd that the 40x was not marked for cover glass thickness like the 60x is (see photos earlier in thread). Since it is not marked NC I figured that it is "understood" that the glass is needed. Of course I had to try it without the cover glass, and got the typical distorted image one...

... Could it be that one of the objectives is giving a little less magnification, thus a larger perceived FOV? I didn't think of that until this morning. . Bear in mind that 160mm is a mechanical tube length, and therefore [depending on the optical designer's preference] the intended primary image ...

Thanks for that reassurance, Tom

MichaelG.

... what do you do concerning that 100x objectives are supposed to be oil immersed?? I really do not know, just asking. Stan . Stan, According to the notes that I linked in a previous post ... Immersion Oil can be applied directly to a dry specimen of Blood, with no coverslip. I haven't tried it [y...

This glass glue may work better than nail polish as sealant: https://www.hobbylobby.com/Crafts-Hobbies/Basic-Crafts/Glues-Adhesives/Glass-Bond/p/28110 Or it may even be used as mounting medium. It dries quickly by itself to a glass like clarity ... . That looks a useful material, at a very good pri...

Work that good is never tedious to watch, John.
.
MichaelG.

Delightful
[please forgive the pun]
.
MichaelG.

I've just been 'googling' and found this:
https://www.titantoolsupply.com/uploads ... oscope.pdf

I have absolutely no knowledge of Titan or its products, but the spec. sheet is certainly interesting.

MichaelG.

As promised : My Nachet stereo-zoom has 10x eyepieces The tubes are only 27mm diameter, which must restrict the field somewhat. Its Zoom objective is nominally scaled 1 to 4 A quick check reveals object field diameters between about 24mm and 6mm It does give excellent quality views, but I don't thin...

lobsters15 wrote:I am sorry I don’t have numerical data.

.
No problem ... I'm sure we can get there if people measure their various 'scopes.
I can check my Nachet stereo-zoom tonight.

MichaelG.

Some numerical indication of what you consider to be a 'fantastically wide' and/or 'disappointingly narrow' field of view would be helpful. I find it convenient to print a few concentric circles of known diameter, for estimating the view at various magnifications. ... I will try attaching a PDF of m...

zzffnn wrote: HI = homogeneous immersion.

.
Thanks for that 'Pearl of Wisdom'
The day is always better when I learn something.

MichaelG.

Great work, John

Lovely sections and helpful description of the work.

MichaelG.