MicrobeHunter.com Microscopy Forum

MichaelG. wrote: ↑

Sat Dec 26, 2020 9:52 am

micro wrote: ↑

Sat Dec 26, 2020 5:17 am

With my setup I don't use additional optics. I have my 4/3 camera attached to the trinoc port with just a tube that has no optics.

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So you are effectively using the microscope objective as a macro lens
... do I understand correctly ?

MichaelG.

Oh I don't really know actually.

If you got a panasonic g7 body only $450, high speed memory card $100, elgato cam link $110, camera adapter attachment $50 it would cost about $700-800 and you would basically have my setup.

With my setup I don't use additional optics. I have my 4/3 camera attached to the trinoc port with just a tube that has no optics. These are screen shots from videos I took with my lumix g85 http://www.microbehunter.com/microscopy-forum/viewtopic.php?f=6&t=10810&p=90632#p90632 and here is a video 74...

If you want 1080 and no lag while live viewing you need a camera $400+, Elgato Cam Link for connecting to monitor with hdmi cord for live viewing $100+, adapter camera attachment prices vary $50-100? Additional optic possibly required depending on camera $50-100 You can get live viewing with just a ...

How much are you willing to spend? 1080p will be a few hundred $ at least.

It does look like a stentor

Shouldn't the sem image have a flagella? The photoshop coloring is really good.

2nd one is a eranema or euglena or something similar

Ya I'll report back after I try the legit swab kit

I haven't used the swab kits yet but I ordered one the other day. The issue with cleaning the sensor is any liquid leaves drops which are visible via the microscope. So you have to clean it with the solution and then go back over it with some sort of sensor friendly dry swab about 100 times till the...

Looks like cyanobacteria but kind of hard to tell without a closer up image

I have the same issue a lot of the time. Stacking seems to create big thick lines making the subject look like it was outlined with a pencil. I think its just an issue with microscope resolution and such and maybe photoshop not really getting how to deal with microscope images or something. I don't ...

If you can find a pond with fish in it that will be good, fish means there's an ecosystem and a pond = still water. Streams don't have much to look at because the flowing stream stops microbes from congregating there. They get pushed farther down till the stream ends.

looks like a weird shaped hypotrich

You can get it to be pretty much perfectly clear. In my experience its easy to get the microscope parts spotless but cleaning the camera sensor was a nightmare from hell but doable.

Planning to upgrade to DIC in the near future. From what I've read there are different types of DIC reflected vs transmitted. Reflected is better? I know I need PlanApo objectives. I've been browsing ebay but I don't really know what I am looking at. I guess I need the slider, prisms, condenser? Wha...

I think the 3rd is a dinoflagellate

I assume just the adults lay eggs but it doesn't really look like waste to me but idk

Not normal

Did it lay an egg?

Yes its a ciliate. The algae looking material is probably parts of other organisms it ate that are being digested.

First one might be the predator loxophyllum

Plasmid wrote: ↑

Sat Dec 05, 2020 4:02 am

The level of detail is amazing, how do you achieve such of nice resolution and contrast?

Larvae are big which makes them easy to image. The sony 6300 probably has decent sensor though. This is also a stacked photoshop image.

Cameras have different magnification factors depending on sensor size and a few other things but the image size will be fine. These kinds of usb cameras have varying results and can be kind of a gamble to buy. The megapixels don't really matter but the quality of the sensor does.

How does the specimen look with your eyes when you use the eyepieces vs the camera?

Need more info on camera specs and more comparison pictures of default non oblique images and such

I've never heard of cyclops dominating pond water. I have them in one of my pond water jars and all the other organisms are fine.

Plasmid wrote: ↑

Tue Nov 03, 2020 5:01 am

Is there any specific type of bacteria growth you are seeing, have you been able to perform a culture or gram stain?

In my jars I just take samples and see thousands of bacteria of all kinds.

20x is hugely worth it

I have had about 10 or so containers of pond water and some of them seem to have had quicker bacteria growth that seems to kill off other organisms. But I do not know what conditions caused some containers to be more ideal for protozoa and others bacteria. Is there a strategy to keep protozoa alive ...