MicrobeHunter.com Microscopy Forum

Most photographic filters are circular polarizers. They did make some linear polarizers, too, but they are rare. Linear is typical for this application. If you use circular, I believe the “front” of the filter needs to face the specimen for best results.

The color is most likely Methylene Blue that somebody spilled. My AO 10 has a very similar ring, but purple; probably Crystal Violet. Any mirror will work, but I suspect the original was a very thin 1st surface mirror for the highest efficiency. (They have silver on the surface so the light doesn’t ...

I'm still very new to this, so please feel free to offer suggestions. Stack of about 20 frames. AO 10 using 20x 1022 and 214F DF condenser.

20200608-stack 5.jpg by Mickey, on Flickr

Great images. Standard brightfield?

Apochronaut certainly knows more than anyone here about these objectives. I wasn't able to find a single post where they were all listed, though. I put together this table from all the sources i could gather. Hopefully it will be helpful to others. If anybody has additions or corrections, please let...

I believe I’m spoiled by P.S.Neeley’s encyclopedic collection of AO information. Is there any resource where one could find a reasonably complete list of the more modern 45mm parfocal Infinity RMS objectives sold as Reichert, Leica or otherwise for the Microstar IV, Diastar, ATC2000, etc?

Most 3D prints will not survive high temperatures, and definitely not parts made on the machines typically owned by hobbyists. (Makerbot and the like)

I decided to put something interesting on the ‘scope for a change. Obviously, there are lots of things yet to learn.

I have a question for something you can try. If you put the 437 into the side of the optical tube with the diopter, how does it work paired with a 176? Aside from not being parfocal with the 176 (it’s wound to its lowest, and I didn’t try to match focus) it looks fine viewed by eye. I am surprised ...

I didn’t note the particulars of the set up last night, so rather that guess, I reset the camera and shot a couple more. This time the spacer between the phototube and #437 was shorter, so the extremities have been cropped out. The distance from the trinoc shoulder the the top of the eyepiece is 155...

The first noticeable thing is that both are displaying a bit of asymmetry. There is a falloff of corrections in the lower left corner of each image but in the second image it is worse. Something is out of alignment. How were these taken? It seems that you are capturing almost the entire image circl...

This is a very quick test of 2 different setups. One uses the #437 as a relay lens, and the other uses a 2X teleconverter. Aside from slightly different framing, can you see significant qualitative differences? Is one better? The “subject” is a laser print. I wanted something that would be reasonabl...

The interior is quite spacious. I may flock it, regardless, once I have some flocking material on hand. After experimenting with the set-up, I might not end up using this tube. With the 10x 437 eyepiece, the area of coverage is too small for my liking, even on a full frame sensor. Even a 5x eyepiece...

Phototube ready.

Yes. If one of those older condensers is just marked 1.25, then it is a two lens abbe. The 1087 is also a two lens but the bottom lens is noticeably aspheric. I suspect that the front lens is the same on each, since the 1.25 N.A. specification is established by it's angle, so theoretically an inter...

If you are a photographer, I suspect the 3mp camera will leave you unimpressed.

Thanks. I will look into the Falcon tubes. I was going to start the acid processing and need to do it outside, since I don't have a fume hood, but the weather will not cooperate. It's suppose to rain most of the day. I want to start with the HCl 30%, to get rid of any calcium carbonates and any met...

I now have a 1043 trinoc head and there is a Nikon T-mount adapter on its way. The phototube is the last piece I need. Today I realized that my condenser is a simple Abbe. It’s only marking is “N.A. 1.25” so I believe it’s a 1088, but I’m not certain. For reasons I can’t explain, I happen to have a ...

LCD's need polarizing filters to work. You can't simply remove them and expect it to behave the same way. Here is an example:

https://www.youtube.com/watch?v=ydRZkLK7S-w

I spotted this on fleabay and it piqued my curiosity. All the components seam to be high quality, though I’m not experienced enough to guess about condition. The Zeiss objectives on a Nikon stand/head is the oddity. I understand that Nikon objectives should be fully corrected, hence the head should ...

Ok, now I'm curious. What would the procedure be without a phase telescope? Eyeball it and call it good because that means you probably aren't doing phase anyway?

Well, I haven’t picked up a head yet, but I did snag a #437 to use as a projection eyepiece. It seems like that is the safest path for high quality images. On a loosely related note, I was wondering about tube lens compatibility. If a 410 Trinocular head popped up, how far off is the correction when...

I searched eBay for American optical trinocular and the first. hit was a 1043 head for 60 or best offer and then a 110 scope for a 100 I saw those, but the condition based on their pictures didn't give me a lot of confidence. There are others, too, for not too much more. I'm still weighing options....

I dropped you a PM.

Just curious, but would a 110/120 Trino head be an upgrade on my series 10? With the right eyepieces it would give a wider field of view, right?

apochronaut wrote: ↑

Mon May 11, 2020 11:09 pm

All of those possible camera set ups for the AO system are there; it's just that the telan lens is an integral part of the highly corrected nature of the image.

Thanks for your reply. I am genuinely grateful to have your AO expertise here on the forum!

You can use one of those but they intercept the beam in advance of the telan lens, which has quite a degree of input into the correction of the final image. In that case, I should probably hold out for a proper triocular head. There isn't much point in going to the trouble of a splitter to then mou...

Hobbyst46 wrote: ↑

Mon May 11, 2020 9:32 pm

Is your microscope an AO 10 ? the title says IO10...

Corrected. Thanks!

I’m enjoying my new-to-me AO 10, and starting to get more interested in using it for photography. It came with the binocular head. My original plan was to use my iPhone or Nikon J4 (1” Sensor) through the eyeport. I made an adapter for the J4 and have played with it briefly. Because it’s not a paraf...