MicrobeHunter.com Microscopy Forum

25x and 40x, 40µm, Brightfield, Oblique and Phase, single images in Photoshop. The unusual oblique effect is achieved by placing a Phase III ring under a Phase II objective. Believe that is a version of COL (Circular Oblique Lighting) http://i1368.photobucket.com/albums/ag171/mapologo/A23w_zpsjvsnhu...

Nice images.

My favorite is #2 because of the wonderful repeating pattern.

Makes me wonder if they are fractal.

Striking images!

Thanks gekko.
No idea who or what. It caught me by surprise.
Evil ? Perhaps it is just as Darwin said, only the "horribly cruel work of nature!"
Not sure I want to clean that slide!
Phase, 25x and 40x * 1.6 which would make it 40x and 64x

Thanks gekko.

How did you convince him to sit still?

Luck. It got trapped in that position by the cover slip!

Now that I have seen it again, I think this is more of an unusual situation rather than an unusual beastie!

If you haven't come across this forum yet you may want to take a look before you "invest" $4000 in anything!

http://www.photomacrography.net/forum

Many thanks Dennis and vasselle.

Did you get from a lake?

Collected from the surface of a friend's garden pond, with a couple of weeds thrown in.
Forgot about it for a few weeks, there is now some detritus on the bottom which has become Ostracod territory!

+ a shorter version which I have slowed down to 25%.

Excellent image. Always adds to it when someone is home!
I am going to go out on a limb and suggest it is nebela-carinata.
http://www.arcella.nl/nebela-carinata

16x/0.35 Objective, 315µm, Brightfield, Oblique, Polarized and Darkfield.

Thanks Rodney. Indeed, I also found the Polarized illumination quite revealing. Hi gekko, many thanks. I should have anticipated the possibility of Ostacods even though it was a new sample jar, since I got the sample from the bottom. They always seems to catch me off base. I tend to use a larger dro...

Thanks mrsonchus, very kind.

Hi shawngibson, welcome to the forum.
6.3x objective x 1.6x Optovar magnification changer x 10x Ocular. 100x total magnification.

6.3x * 1.6, 550µm, Brightfield, Oblique, Cross Polarized (Full and Partial) and Darkfield, 6 image stacks in Photoshop. http://i1368.photobucket.com/albums/ag171/mapologo/Ostracod46-51_zpsbu4wowuw.jpg http://i1368.photobucket.com/albums/ag171/mapologo/Ostracos37-42_zpscdsexqul.jpg http://i1368.photo...

Same manual. First is a scanned original, second one has been retyped.

https://goo.gl/6icdt6
https://goo.gl/0JFS7V

Given its size, the first three images are impressive. Nicely done!

Beautiful images.
Wondering if they might be trachelomonas?

Can't see it ! Might have something to do with the [/img] at the end. Just tested it, bracket needs to be on the same line. Only problem then is that the image is not of a Pediastrum but of the mystery "thing".

These are Zeiss instruction leaflets for 3 of their 4 DIC designs for 160mm tube microscopes. + extract from the Zeiss Universal Manual on the use of the 4th DIC model, as I have not located an instruction leaflet as yet. Note: 2 are for reflected DIC and the other 2 are for transmitted DIC The 4th ...

Very nice images. Thanks for including so much detail - I will try to do likewise when I next post.

Went back to the jar and got a fresh sample so here it is.

How well did the glycerine work the second time? Can you give us a idea of what was too much?

I agree with Charles. Both illuminators provide Köhler, as long as you can get the bulbs you are good to go with either one. I would guess that the ortho illuminator once set up will require less fiddling about. While the spencer 735 will probably be a little more fiddly, it is black and has a cool ...

The link I provided has several catalogues, the one called Zeiss_prices_7-1-74.pdf includes the Objective in question. 46 19 20 is a 63x/1.25 Oil Neofluar as I mentioned initially. I think you will find that the 63x/1.25 Oil Neofluar is listed as 46 18 20 I believe the 99-- suffix is for newer/later...

The link I provided has several catalogues, the one called Zeiss_prices_7-1-74.pdf includes the Objective in question. 46 19 20 is a 63x/1.25 Oil Neofluar as I mentioned initially. I think you will find that the 63x/1.25 Oil Neofluar is listed as 46 18 20 I think the 99-- suffix is for newer/later b...

According to the specs, the illumination is 6v/25w. Should be alright even if it is plastic.

http://www.lomoplc.com/AL3000.htm

I agree that Boris is a definite step up.

Nice image. That is some seriously large alga!
Re - Amoeba: if those are Pseudopodia they are remarkably regular. Seem more like tendrils.
Did you manage any closer views?

Amazing images. I second gekko, details please!

Very nice. What is the NA of the 20x you used for darkfield?

No problem. Here is a link from the Resources (online, books etc.) section you might find interesting :

viewtopic.php?f=15&t=773

Did you use an additional filter or can DIC produce such colours?