MicrobeHunter.com Microscopy Forum

I echo Gekko. So Mrsonchus, what is your serious hobby :) ? BTW, I would be interested in your experience with; "I've also been working on a slightly enhanced image-stacking method that has really yielded good results (i.e. an improvement in my own stacking results) - this is very recent (yesterday...

That's about it at the moment, This has got to be the understatement of the century! :) Ha! Gekko nice to hear fro you - yes I suppose it's actually quite a list, there's just so much that's intriguing & fascinating, here's a quick look at a stitched pair of stacks of the vascular-bundles near the ...

Hi John, Have you got anything new to post?... You have started a very interesting thread!.... The Best, BillT Hi Bill, yes I've been working on a couple of areas of my workflow; I've been investigating the tendency of some sections to have areas that 'ruck-up' in the manner of a carpet on a slippe...

Welcome Ken, from the UK branch! You'll enjoy this fine forum, many very friendly not to mention talented folk here - a great place to share your adventures and experiences! There are folk here that can tell you just about anything you'll ever need to know - a very experienced and capable group from...

Excellent images, especially like No.1. It's very interesting and useful to see the exposures used too - it is a handy 'yardstick' just to give some idea as you say, of 'how much light' is being used - I often totally ignore this aspect of my pictures which is of course less than ideal. Your post is...

That's a lovely, supremely well-defined picture, excellent! Interesting to see what your culture yields too.

einman wrote:

mrsonchus wrote:Have you considered hydraulics at all as an advance-mechanism, or even heat-induced rod expansion? Just a thought for extremely smooth & fine control?

Yeah that's all I need another Hobby!!! LOL

Just trying to help fellow, just trying to help...

Have you considered hydraulics at all as an advance-mechanism, or even heat-induced rod expansion? Just a thought for extremely smooth & fine control?

You used an objective from a microscope - you've made a 'microcamera' - definitely a bona-fide addition to this forum I'd say! :D Orientating the beasties may possibly be helped by 'sticking them down' - perhaps some double-sided clear tape would enable you to literally manipulate their tiny parts t...

Super Seb, very nice and informative pictures! What did you do to get the tissues to this position?

A curious term Bill, we're all surely merely perusing and sometimes engaging in this forum's threads are we not? I never considered myself or anyone else to be 'lurking' - a public post on a public thread is, well, public....

Hi Rodney - the vignetting - forget all about it, it's a distraction to you and is only getting in the way of your progress and enjoyment of your photography and image-sharing activities, it's going to happen all the time with a hand-held point-and-shoot simply held up to the (an) eyepiece of a ster...

Beautiful picture, so sharp - fabulous to the eye aesthetically too!

I don't have to be able to take photos to use a stacking program... If some one supplies me with the step images, I can stack them... BillT True enough Bill, that stack from Picolay is very impressive, I'd definitely say a little better than the Toupview version I posted. Do you not fancy a Toupcam...

Hi Rodney, this I would say is almost certainly a camera-stability problem, this isn't what one would usually term 'vibration' but movement far in excess of vibration.. At these relatively low magnifications vibration from for example pressing a camera's shutter-button would not in my experience cau...

Hi, interesting but very little detail to go on - need a few good pictures under a 'scope if possible. great idea, how did you go about the 'culture' process with the petri-dishes, medium and 'plating' process as you called it/ I'd like to have a go at something along these lines in the future. :) p...

Hi Rodney, not bad but you may have reached the limit of the technique you are necessarily using. Your main priority really needs to be, as others have already said, the mount of your camera - it absolutely must be in exactly the same place (i.e. have not moved even 1/100th of a mm) for each compone...

Thank you lot for your replies, mrsonchus and billbillt ! The root pieces were grown as a whole plant :) and were separated just before the experiment. I was growing plant callus and sterile plants cultures some 10 years ago. I had experience growing lilacs, plums and apples in vitro (at that stati...

billbillt wrote:Hi Naptha,

Here is the link for Picolay...

http://www.picolay.de/

BillT

Hey Bill, that's a very handy link - I'm definitely going to have a look at Picolay too - you can never have too many options in microscopy! Thanks for the link and screen-shot.

That's a very nice, crisp & clear result! :) Here's 'Toupview's' 'maximum-contrast' stack option of the same pictures, with default parameters... ws_root_maxconstack_default.jpg Not bad... :) I would say, comparing the smallish images we have here, that Picolay has slightly sharper image? Hmm - let'...

I'd say a wing-scale - certainly looks like one and is the right size...

Hi Naphthalene, nice set of pictures, have you had a look at the root pictures I recently dabbled with, sectioned on the Shandon? I'm interested in the way the lateral-roots originate within the main root.. Anyway, I always use a totally-free program that 'works' my Toupcam USB microscope-camera, ca...

Hi Rodney and well done with your 1st attempt - you're off to a good start! It's not easy to stack when you need to hold a camera in the exact (virtually impossible) position for each stack-'slice' amd you've clearly had a good effort at it - well done. Keep practicing because you will definitely ge...

Arggghh.. that is ghastly!

Nice images both! :) They really are inseparable with regard to their stacking results. Both are a really nice tone too, did you stain with Iodine? I compared several stacking programs (free ones I hasten to add :) ) and had the same result across the board, combineZp, jstack (something like that - ...

Hmmm.. I'm not sure what's what in this one? Which part is the fly's 'tongue' Rodney?

---------- Is the upgrade from the stock objectives to the Plan Achromatics significant and worthwhile ? Thus I included the Novel into consideration, considering the Novel comes pre upgraded with plan achromatics ---------- Hi and welcome. Personally the change I made from 'ordinary' objectives to ...

Sheer beauty and a demonstration of how it should be done! Astonishingly good!

Thanks, John. Simply due to the sheer volume of them around, I think I can get an AO 820 for very little. I guess they almost dominated the market for quite a while. I will be asking questions for sure. The help is appreciated. You're very welcome apo' The AO 820 looks perfect to me, virtually the ...

Thanks for that detailed answer. I certainly makes shopping a lot easier. I had considered a sliding microtome , but what you are indicating is that it becomes difficult to maintain precision on the less automated designs. I'll have to re-think that. I guess Shandon has become one of the major make...