MicrobeHunter.com Microscopy Forum

Hello RobBerdan,
impressive photos, although I love to see Volvox in its natural colours.
Cheers,
Hans

@75RR
definitely a foram,
see my posting viewtopic.php?f=6&t=5818
Cheers,
Hans

@bob
I have sent you a PM
Hans

Hello @MicroBob,

originally I thought to keep the platform above water level to keep it moist

Why don't you let your platform swim in a defined immersion depth?
Greetings,
Hans

Hello, interesting thread. I have a similar project and wonder if anybody here has any idea: Recenctly I have obtained a handful of bog sphagnum inhabiting several species of testate amoeba. Sphagnum are multi storey buildings with organisms in the bottom region requiring pure water, up to some in t...

Hello forum, Heleopera petricola is not uncommon in wet bog sphagnum. Unfortunately water samples from this source usually are quite untidy, with sphagnum leaves and other debris spoiling the view. So it is a challenge to view a tidy specimen. Here is a nice 135 µm high specimen: http://www.hans-rot...

Hello 75RR, I believe your amoebae belong to the genus Zonomyxa. I had based my guess on the countless small (< 1 µm) violet granules filling the cytoplasm. There are two very similar Genera: Amphizonella found in dryer moss. Zonomyxa living in Sphagnum. (see also http://www1.nencki.gov.pl/pdf/ap/ap...

Hello 75RR,

I believe your amoebae belong to the genus Zonomyxa.
See also http://www.mikro-tuemplerforum.at/viewt ... 625&p=2064

Regards,
Hans

Hello Grahame,
cannot figure the size oft he creatures from the numbers in the pictures, 30 µm and 20 µm cannot be the diameters?
I believe these are Arcella of the hemisphaerica/rotundata/intermedia-Komplex, sizes between approx. 35 and 62 µm.
Greetings,
Hans

Hello Aenima,
I believe it is Zonomyxa violacea or an Amphizonella species.
Cheers,
Hans


Added: Se my http://hans-rothauscher.de/testaceen/pyxidicula.htm

Hello @desertrat a scientific paper Thanks, I did not know this paper. Interesting that testate amoebae predated the Ediacarian. I have read somewhere that the filopodia (Eyglypha) are still considerably older than the arcellinida. Also interesting that Testate amoebae have been successfully used as...

Addendum:

@ Charlie Guevara
All Hyalospheniidae are quite flat, see



Hans

Thanks for your comments. The collection and subsequent use of xenosomes, i.e. foreign materials for the reenforcement of testacea shells has frequently raised the question: May this already be called behaviour? Many members of the Hyalospheniidae build their shells using recycled shell plates scave...

Hello, ths is the 169 µm high testate amoeba Planocarina (former Nebela) marginata obliquely illuminated: http://www.hans-rothauscher.de/testaceen/forum/marginata.jpg The shell is covered with scales which had been robbed from Euglypha and other foreign objects. 40x Neofluar, Stacked image, contrast...

Hello RR,
My wife collected them from Mombasa beach. The ones I measured were between 250 and 400 µm.
Greetings,
Hans

Hello, here are some Foraminifers from Mombasa Beach, Kenia: http://www.hans-rothauscher.de/testaceen/forum/foram11.jpg http://www.hans-rothauscher.de/testaceen/forum/foram12.jpg http://www.hans-rothauscher.de/testaceen/forum/foram6.jpg http://www.hans-rothauscher.de/testaceen/forum/foram9.jpg Speci...

Sorry Kurt for wrong spelling.

Even the word hyalinity, I fear, does not exist and is my own word creation.

When Arcella cells are young, their shells are are colorless and hyaline, i.e. translucent, older ones become brown and intransparent.

Sorry for confusion.

Hans

Hello Hakan, they are all great. I like the Arcella best. I believe its a young Arcella gibbosa, when they grow older they partly loose their hyalanity. The blob bottom left of the sarcode is a contractile vacuole. By osmotic pressure the organism constantly absorbs water. The vacuole pumps excessiv...

Hello,
this is an insight into a 120 µm wide alive Arcella mitrata:

One focal plane. 60x achromat.
Cheers,
Hans

Hi HKV,
I like best the first Ceratium.
Hans

Hello John B, yes, a lot of very useful information for me to start with, thanks. At present I use a Toupcam-cmos-camera with the adapter FMR 050 and the great Toupview software which came with it. Here is my present tool, and I do not want to miss the pretty Zeiss KF2, and I very much like the conv...

Hello,

Which cameras, DSLR or compact (micro four-third?), can be controlled via the laptop, i. e. tethered shooting? Until now I only found high end Olympus like the E-M1 and the E-M5 Mark II.

Thanks,
Hans

Thanks Jim,

No DIC, basic brightfield using achromats.

Greetings,
Hans

Hello,

this image shows the honeycomb structure of the shell of a live Arcella cf. vulgaris, 102 µm dia, dorsal view.
Stack of 25, 60x dry Achromat:



Cheers,
Hans

Hello Mr. Micro,
it is a testate amoeba, definitely a Centropyxis species most probably C. aculeata.
Cheers,
Hans


See https://arcella.nl/centropyxis-aculeata
(edit this link added)

Hi Francisco,
Thanks, interesting! How the plasma moves to and fro between the two shells, until both have an equal share and composition of plasma and food particles.
Hans

Thanks Jim,
these were two individuals on same slide. A matter of good luck.
Greetings, Hans

Hello, this is the rare Arcella formosa ( about 200 µm wide). It has so far only been found in a few locations in Canada, Netherlands, Hungary, Germany (Mecklenburg) and this year by me in a nearby fen: http://www.hans-rothauscher.de/testaceen/forum/formosa8.jpg (dorsal) http://www.hans-rothauscher....

Hello Francisco,
Very interesting, only saw them once.
Was this in fresh water?
Hans

Hello Ronald, I only found them in one place, where 3 years ago they suddenly appeared in great numbers. There still are half a dozen in each petri-dish from recent water samples. This is a muddy ditch in a fen with birch and alder. Water and ground are slightly acidic (PH ~6), no real bog. Some sph...