MicrobeHunter.com Microscopy Forum

I’m not sure how relevant this might be ... but it contains some brief technical details, so seems at least worth noting: https://theskysearchers.com/viewtopic.php?t=4108

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and here’s a useful snippet from the Ortholux II manual:
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MichaelG.

LouiseScot wrote: ↑

Sun Jul 26, 2020 7:55 pm

... I still don't see any for sale

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Just to show that they exist: Here is one on ebay.uk

https://www.ebay.co.uk/itm/Nikon-Micros ... 3463180519

MichaelG.

I could try adding a Nikon CF N 160mm objective (if I could get hold of one!) but then I presume I really would need matching compensating eyepieces and a Nikon compensating photo eyepiece? . Absolutely categorically not needed The whole reason that I suggested using a CF objective is that they are...

Since I doubt that you are picking up the intermediate image where it should be, I would stay away from Nikon CF objectives. They are pricier and you would still need to use an eyepiece to move the image from the trinocular tube to your sensor. . Viktor, Sorry if it was not obvious but ... given Lo...

That’s a superb quick-reference document, Carl

Thank You for producing it

I have taken the liberty of suggesting it be linked on the Manchester Microscopical Society website, as recommend reading.

MichaelG.

You are possibly/probably missing the performance that comes from using an objective within its design envelope. […] I don't know, because I haven't seen the specification of what you are using. MichaelG. It's just a cheap trinocular scope which probably cost in the region of £300-£400 (I can't rem...

I'm afraid you're confusing me - I've always imaged on my old finite scope with a camera, trinocular tube and plan achro objectives up to 60x. There was never any other option. What are you saying I'm missing with my current setup? Louise You are possibly/probably missing the performance that comes...

... images via the trinocular tube with no other optics involved - just camera sensor and objective. Louise I can't leave this discussion without mentioning: That is a very bad place to start. You are effectively using the objective as a 'macro' lens This is only viable with a few [typically simple...

Hi Just looking at percentages may be an oversimplification. There are different types of aberration. But, for my purposes, I'm really only interested in improving rather than reaching a theoretical optimum :) Louise It's certainly a simplification ...but I thought it might be a good place to start...

I think I was just saying that with a finite tube microscope, and all else being equal, the quality of the image will be mostly determined by the quality of the objective i.e. apo objectives outperform achro ones. It would be strange if they didn't! Louise O.K. I'll buy that ... but look at this wa...

Yes, I've seen that. Obviously with a finite tube the camera sensor and objective have to be aligned and there will be chromatic aberration with an achromat - which is my point. Louise . Sorry but I simply don’t understand your comment ... david_b is having problems with an infinity corrected micro...

LouiseScot wrote: ↑

Sat Jul 25, 2020 12:06 am

Surely the optical quality is down to the objective.

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Louise,

May I suggest that you have a look at the concurrent thread by david_b

viewtopic.php?f=5&t=9968

MichaelG.

https://moticeurope.com/en/25x-photo-eyepiece-to-be-used-with-photo-adapter.html ... looks the same I admit to being a little bewildered . . I'm not sure what a 'K' version is but the photo tube and eyepiece were supplied with the microscope by a Motic dealer. It looks like a generic Chinese optic.....

https://moticeurope.com/en/25x-photo-ey ... apter.html

... looks the same

I admit to being a little bewildered

MichaelG.

Ouch ... that’s very disappointing, David First thought is that the photo eyepiece must be ‘correcting’ for chromatic aberration that wasn’t there in the first place ! ... if you see what I mean. MichaelG. . Edit: I’ve just checked the Motic site, and confirmed that your ‘scope is ‘ CCIS ’ .. is the...

I have no connection with Mayo Clinic ... but this is a good description of ‘floaters’

https://www.mayoclinic.org/diseases-con ... c-20372346#

MichaelG.

Zuul wrote: ↑

Thu Jul 23, 2020 7:20 pm

Would putting a few saline drops in your eyes before a viewing session help flush the floaters out?

‘fraid not ... they are inside the eye, in the vitreous humour.

MichaelG.

keithstout wrote: ↑

Thu Jul 23, 2020 5:07 pm

I have annoying floaters in my eyeballs as I look in my microscopes.
[...] but does every microscopist experience this?

Keith.

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Very common I'm afraid, Keith

Experiment with your illumination settings until you find what works best for you.

MichaelG.

MicroBob wrote: ↑

Wed Jul 22, 2020 5:42 pm

Hi Håkan,
great images, a lot of effort well spent! And thank you for pointing out the crucial points of the method. Everybody knows that peacock feathers are beautiful, but not that they are this beautiful.

Bob

+1 from me ... and one more from my wife !!

MichaelG.

This could go under so many topics ... so I’ve chosen ‘Miscellaneous’ https://www.smithsonianmag.com/smithsonian-institution/Elusive-Ultra-black-Fishes-Are-Cloaked-to-Survive-the-Deep-Ocean-180975367/ MichaelG. . Direct link to the full report: https://www.cell.com/current-biology/pdfExtended/S0960-...

Well, if that's what happens to 47-year-old black anodized aluminum I would venture a yes. . It’s certainly not unknown. MichaelG. . Thought you might like to see this, Bram . 7D6C2287-8FCB-4AB2-82EE-B91FA8D67DF1.jpeg . It’s not an analytical photo, but it gives a pretty good indication of how Blac...

Yes ... that is what I deduced by looking at the datasheet

MichaelG.

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Hobbyst46 wrote: ↑

Mon Jul 20, 2020 8:55 pm

Michael, I refer to pre-BH Olympus microscopes.

Thanks for the clarification, Doron ... I evidently misunderstood what you meant by

Previous era Olympus sub-stages (such as the machined part shown in Kurt's photos)

I’m happy and contented now.

MichaelG.

Previous era Olympus sub-stages (such as the machined part shown in Kurt's photos) were made of steel (not stainless) and very sturdy and strong. . But Kurt wrote: The Olympus substage assembly is made of aluminum, and milling it while simply held in the vise was no problem whatever despite the pre...

The power supply is a dimmable constant current driver . . Very tidy conversion, Brian ... and obviously effective ! That power supply had me confused for a moment though, until I downloaded the datasheet “dimmable constant current” reads like a contradiction in terms; but I now realise that they m...

... Great minds thinking alike, Zuul

I only saw your post after making my edit

MichaelG.

The Olympus substage assembly is made of aluminum, and milling it while simply held in the vise was no problem whatever despite the precarious looking overhang. Useful to know ^^^ Thanks, Kurt. MichaelG. . Edit : Having read Kurt’s response [below] I realise that his “precarious looking overhang” w...

That’s a great ‘special’ you have made there, Kurt

Nothing ‘Nasty Franken’ about it, that I can see !

MichaelG.

If you are talking about ‘tube length’ DIN standard is 160mm

MichaelG.

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This is a convenient reference:
https://www.edmundoptics.co.uk/knowledg ... bjectives/

Welcome, Theo !!

MichaelG.