MicrobeHunter.com Microscopy Forum

Wow!!! It's great to see how a simple matter of placing the simple question mark ?, in the right place has turned a simple question of the use of a question mark has turned into a good debating subject. Now I am going to think of another good question regarding diatoms which hopefully will turn in t...

Sorry members I still do not understand why the full 'English' meaning of the topic in question is not written out in full i.e. Genus Testate omoeba species not known members your help is needed please. Please I. D. its species for me Thank you. Or another way might be ; Testate omoeba found but spe...

Why complicate matters why just put Testate amoebae species not known. Members your help is needed thank you.

What do you think its been eating? Looks possibly like the remnants of a diatom perhaps.

Hi Guys, I am sorry to see you are in a bit of a pickle when it comes to sending stuff to each other, (lucky people) I mean if you each send a sample to different members then what a brilliant collection you would all have , and please give a thought for your buddies across the pond, who as yet are ...

Hi Rod, If my pitiful brain serves me well the brown would have been Bismark Brown and the red would have been Carmine that is in the Field Poppy and the Cosmos. What I like about B. Brown is that one can not overstain with it. I now need to look up some of the protocols I used a while back as I wil...

Some really good staining on many of the specimens, do you have a timing in place for certain stains or is it trial and error? What is your methodology as I use a few different protocols when staining my botanical specimens. I found maybe one or two of your photos rather dark with what I would call ...

Hi members, A member of a small group of microscopists has aske me to show them how to make a stained botanical slide. While I feel very honoured I know need to put my thinking cap on regarding wax, stains (strength, for how long to stain etc). I thought I would give a peep at what I have done a cou...

Hey that's great it reminds me of A "Polo the mint with a hole"

I know but there is no cure for what I suffer from.

Members I have downloaded both programmes and have had a dabble into both. Needlees to say I still do not have a working knowledge of how to work either programme. If there is a member who can use and produce good stacks would you please e-mail me privately to give me an idots guide to getting eithe...

Hi guys, I have had this error 403 code on my desk top for about three weeks now, so can someone help me get rid of it please.

Thank you wiz kids.

Mo (exmarine)

Hi All you guys over the pond,

You have been busy and with good results as shown by the photos, also thanks for sharing the methodology for collection.

Really interesting reading the posts and studying the diatoms to come to a conclusion as to the generic make-up.

Thanks guys.

Exmarine.

OK guys what are the bubbles in the first picture?

And what are the brown colouring and brown bubbles in the second diatom.

Bearing in mind they are rough and ready diatoms.

I will post what I think they are, easy really.

Lets go guys.

Great photos and by showing them in this mode it is relatively easy to give them an ID. I do not have my diatom books with me so will have to wait to find out the ID of them for personal interest only. Not that I am disputing any name given by you rnabholz.
Keep them coming.

Wish I could join in with the debate but I am in hospital having my fourth knee joint replacement done in Cardiff, Wales, and I live in Cambridge, England. The reason for this is because the top surgeon for sorting out other surgeons cock-ups is based in Wales. Enough of my woes members keep the dia...

Hi Guys,
Photograph 3 I think is Rhaphoneis nitida which is a marine diatom. As for the others I am still looking.
Hope this helps.

Hi billbillt was there any need for that comment?

For what it's worth I think its Pinnularia intermedia I am not disputing that it could be er......... the other one what's it called? Oh yes Surrirelloid sp. :lol: A bright field photo would certainly help with the ID. Keep them coming they are a pleasure to look at. Thanks. I do not know how this h...

For what it's worth I think its Pinnularia intermedia I am not disputing that it could be er......... the other one what's it called? Oh yes Surrirelloid sp.
A bright field photo would certainly help with the ID.
Keep them coming they are a pleasure to look at. Thanks.

Hi billbillt OK on your recommendation I will download Picolay only if you supply me with an absolute idiots guide, because this is one big idiot your talking to.

Members I have downloaded Combine Z to try my hand at stacking and hopefully producing a better class photo.
Is there any member who would write down an idiots guide for me please. Step by step.
Thank you.

Very nice photos of the diatoms, I think the Amphora is A.veneta but to be positive it needs to be studied in bright field as in your photo the raphe is not clear. I hope I am not being too picky, as for the other diatoms great photos but as I have said before not good for identification purposes. T...

Fabulous photo Johann, it reminded me of another testate amoeba named 'Euglyha' which has a few spines and is a very common one.

Looking through my small "Field Guide to Mushrooms and Toadstools" I have come across the actual fungi its Coprinus xanthotrix.
So Rylander you certainly know your fungi congrats my friend. I have taken a couple of shots of how they are decaying.

Hi Rodney, Sorry my friend but I do not have the mite stashed away somewhere I gave it a reprieve from a watery grave and placed it in my flower bed. I have no idea how it got to be on a single clump of pond weed. Oh well all's well that ends well. I just wondered what species the little thing is /w...

Members whilst looking at a sample from my black bucket I saw a small black snail on a tuft of pond weed and then something moved just above it. Blow me down with a feather its a Mite. How the dickens did it get there? All it could do was to move from one floating piece of duck weed to another. I am...

Right members I think I have the answer (I could be wrong) but I would put my shirt on it being Biddulphia this was obtained from the 'diatoms of the inland waters of temperate North America' it also states Solitary or in straight or zigzag chains. Mostly Marine and Rare.
That's all folks.

Hi Rob,
It would be great if we could come up with the answer would it be possible to show the said diatom in normal brightfield? Then I would study it until I come up with an answer (right or wrong).

By the way I do not know the area you collect but is it mainly Marine environment and not freshwater? Because Pleurosira laevis in my book is a Marine diatom. The round diatom with ears I think you'll find is Kittonia elaborate (not in my Atlas of British Diatoms) On the whole a good evenings work c...

I have a feeling that is definitely a type of bacteria, don't know what type.