MicrobeHunter.com Microscopy Forum

I sometimes use the Nikon 1J5 on microscopes, but in an afocal setup with the 18,5mm objective over an eyepiece. I don't think it works well without a Nikon camera objective mounted, it might not even release. Rolf Vossen has developed clever camera adaptation solutions for Zeiss, Leitz and Olympus,...

Leitz optics don't tend to delaminate apart from one objective. Typically delaminations show a more defined border - but difficult to tell from photos

Hi Oscar, you have to keep Zeiss Jena apart from Zeiss Oberkochen/Göttingen. These were separate companies between about 1945 and 1990 and went different ways technically. One of the inventions that came with the Zeiss Standard line (West Germany, Zeiss Oberkochen/Göttingen) was that the objectives ...

I don't think that one could buy a complete Standard 14 with bino tube for 100€. This is probably the most sought after microscope series at least on the german market and there are reasons for this. Here a lot of components are available that are scarce for other systems or not availble at all and ...

Hi Dennis,
Do you happen to know whether this is the same Leuckart from whom the Leuckart moulds (metal angles to build individual paraffin moulds) are named?

Bob

Hi, I use this holder: https://www.thingiverse.com/thing:2562841 It needed quite some optimisation after printing and can't quite compete with a metal version but it works well. I now use it on my KOSMOS student rotary microtome, tiny 6kg model. The holder doesn't put the cassette under undue stress...

Interesting comparison!
Which light source did you use with the R and condenser setup?

It helps a lot to have a dedicated eypiece mounted permanently to the phone holder. The eyepiece has to fit to the used system. For the typical wide angle phone camera a wide angle eyepiece would be ideal. These are eyepieces with comparatively high magnifications and at the same time high fields of...

You probably also never thought that you would ever collect that many nosepieces! :lol: What I find is that I have favourite objectives that are useful in different setups and make me change the setup on the nosepieces, mixing everything up. What i like is to have a separate nosepiece for oil object...

What components do you already have?
- DSLR DSLM?
- third eyepiece fitting to your microscopes optics?
- phone with good camera?

In case it is far from parfocal you use the objective with the wrong distance to the object. In some cases not much happens, in other cases the image suffers a lot.

Is your camera parfocal with the eyepieces?

Hi Neal, you might try to restore the slides by dissolving the mountant in xylene and mount the specimens new with proper cover slips, thin layer of clean mountant. But it won't be easy to get the specimen in place withut tearing or folding it. They may make them this way to save time. Slicing them ...

By the way: Did you use immersion oil with the 100:1 objective?

This looks very promising and you already found a nice diatom colony! In well serviced state the focus of this microscope work well dampened and and smooth. The grease deteriorates and either binds up or dries out. The microscope won't die from being used in the state for a while as it is a very bul...

A typical characteristic of microscopy is that you need different equipment for each new topic, and usually there is something needed that is unavailable, expensive, dangerous or must not be talked about. :roll: This can lead to a heap of equipment, much more than the instrument itself. With experie...

What do you plan to observe? Stained sections won't profit from phase contrast. If you want to observe plancton life brightfield is a good starting point, adding a little oblique or DIC - lookalike oblique. It also depends whether you want to observe or photograph: I like phase contrast for observat...

Hi, this is a Zeiss Standard Junior in Pol version. It has 160mm tube lenght, 10mm placement of the intermediate image and 45mm parfocal objectives. The AO objectives are probably too short to focus and optically different. The Junior is a very nice microscope, bullet proof mechanics and finely made...

I have a Zeiss West Plan 1. It is a lot longer that 45mm, not parfocla at all. Sharpness and planarity are fine. I also have a set of Zeiss low power condensers, simple lenses with stops. My aplication would be to take overview pictures of big stained plant sections. Unfortunately the color renditio...

Hi Phil, I think Borosilicate glass is neutral or even yellowish in colour. Today I'm not aware of a source for slides from borosilicate glass. There are slides named "Duran" but they are soda lime glass, albeit a better kind. Could you try to find out if your slides are made from borosilicate glass...

I had a look into my tube: It is this typical oldfashioned blackened brass with no light baffles in it. But they may have been removed when the bino tube was attatched. The bino tube has a lens right at the bottom. The physical length of the bino tube is longer than 160mm so this has to be compensat...

Interesting scope. The large tube was important enough to get mention in the literature you've shown - I'm curious what purpose it had? Hi Pete, do you mean the diameter of the tube above the nosepiece? I think it helps to increase contrast by cutting down reflections and underlines the visual impr...

Much of their information is in paper form and they are unwilling to scan it, you can only go there and look at it in person. In the delivery list for their first instruments I found an interesting fact: The first instrument went to Hamburg, my birth town, to a member of the "Naturwissenschaftliche ...

Congratulations, you've got a very nice instrument there! For more information you might contact the Zeiss archive: https://www.zeiss.de/corporate/ueber-zeiss/geschichte/archiv.html I have somewhat older model 53xxxx which I was able to date to 1911 by comparison with information found here: https:/...

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That is a nice spektrum, jjtr1! If this is from a single die it would be very interesting to investigate further. Many microscopes don't have a true Köhler setup but a frosted lens in the light path that acts as the light source. In this case multiple LED dies could be used. Colour enlargers used t...

OK, this LED bulb is efficiency class "G" only : https://www.emos.cz/led-zarovka-classic ... -bila-ra97

That is a nice spektrum, jjtr1! If this is from a single die it would be very interesting to investigate further. Many microscopes don't have a true Köhler setup but a frosted lens in the light path that acts as the light source. In this case multiple LED dies could be used. Colour enlargers used th...

I think fluorescence in the blue-green range would be easy to achieve. But it probably would be too intensive for an overall white light. There are separate blue-green leds that could fill this gap in a controllable way and the white balance could be corrected afterwards. Im no pathologist so on my ...

Hi Louise,
perhaps you may test the heat sink for temperature when running at full power. Did you use heat compound?
As long as I can touch the heat sinks for some seconds I treat them as ok.

Bob