MicrobeHunter.com Microscopy Forum

I have this same setup. The spring-loaded yokes that carry the lenses can be a bit finicky for sure. Say, could you do me a favor? Could take some photos of the photo adapter, particularly the way the mirror is situated on the retractable arm? I have been having alignment issues and while I think I ...

Phase contrast is useful for looking at nematodes which are otherwise pretty transparent. Darkfield also. Phase is very nifty, but you might want to save some of your pennies while learn how to use a microscope as several older, discontinued lines can be found on the second hand market in some cases...

Well, there have been many projection eyepieces made. Which one is yours?

You might try experimenting with several alternates to see if conditions give rise to different mixes of organisms

The trick rex is doing is using coverslips as the slide

The B&L "toaster" and Nikon universal are good old lamps with field lenses and irises. They can be retrofitted with different lamps if you take notice of where the filament should go. The Nikon actually has a helical focus on it. AO, Tiyoda and lomo made some nice looking lamps too, although I don't...

Yeah looks good scratches will cause some loss of contrast in proportion to the total surface area they take up which is not much. My Nikon 10x plan apo.has a chip out of it but you don't notice in use, especially as it's such a low power with a big good size front element

Yes there is a condenser and I've already tried adjusting it. This whatci do when I use my microscope -take the dust cover off -turn on the microscope light -carefully put in a slide and make sure it is latched in -line up the sample That's pretty much it... Well you might try the focus knobs I guess

Hmm Zeiss had a low power zoom objective. B&L had the student zoom scope that went up to 40x I think, and of course the low power flip-bottom lens. I would think it's a parfocal issue, and the user doesn't quite have enough experience to focus the 40x. The range over which a 40x will focus on a thin...

It's not that expensive seems like just a couple thousand could set you up more a wonder why phase is so cheap

Your field lens will collect light coming off the filament and direct it in more or less a straight line to your condenser. The field iris or stop can prevent some glare but also provides a centered surface to focus on when adjusting the condenser to make sure the filament is perfectly unfocused. In...

The field lens is the element that distinguishes Kohler from critical illumination in practice. The irises are useful but secondary.

I don't remember Kohler mentioning epi illumination in his article. You're using the objective as a condenser, so I assume the most important part is getting a field lens after your light source with some adjustability.

Gonna want to look around for some sweetgum trees, the.ones that shed those prickly balls. It might be to late this season, but next spring cut a bit of bark off in the morning and see if can't get some sap. You want to boil this in three changes of water then leave it exposed to the sun the entire ...

3mm should be fine that disc mount must be quite small

Related to the equipment question, higher magnifications objectives need coddling and extra care about centration and the right kind of lighting and oil in some cases or even worse cover slip thickness precision you might we'll find they don't reveal as much as the trusty 20x, greatest of the dry ob...

Drawing has certain advantages, accentuating specific features is one I think. Every once in awhile, one can find a camera lucida to attach to the drawtube or eyepiece. I have an old Spencer so model that is incredibly beautiful, and it works well also. It could be an alternative if you find a cheap...

Yeah those old timers used the most toxic and vibrant stuff that existed looking at Grays big book he's tossing mercury and chromate and picric acid every which way no "hey careful with this it will kill you"s in sight.

Turtox from general biological are good slides for sure.

The theoretical ideal light source is a single point and a field lens of the right size and focal length. Filaments and arcs would approximate these. As the na of your objective increases, the amount.of surface area from your light source you can use.to collect light diminishes, even with an ideal f...

You're gonna want as small a single.die as can deliver the light. The picture looks like an array, which might benefit from a diffuser in some setups.

The reduction lenses sold by Amscope and omax for around fifty bucks are quite bad. You might look for one from optem or spot. There also have been a few reduction lenses, some examples I know of from B&L and Nikon, that incorporate the eyepiece, basically a fixed afocal jig.

(I may have heard it from apo)

I have heard you can use it as a phase telescope to examine your diaphragms

I made a pantograph a ways back to this end but couldn't figure out how to position it without the arm bumping.

I got a nice Narishige clamp I'm going to mount on the end of it to do specimen arrangement with a pick. My hands are too shaky. What would you do?

There's at least one cool trick you can do with a 1x: https://i.imgur.com/hqvPcyT.jpg This is the Zeiss Plan 1x .04. I cheated a little bit as this image is about twice as big as spec thanks to some adjustable drawtube fiddling also I can never be totally sure how much compensation Zeiss lenses need...

Substantially similar to the method of action in the 1931 patent by JH Emerson, of Iron Lung fame: https://patents.google.com/patent/US1828460A/en The idea behind this device was in part to make it easy to repair even for a thick-handed clod like myself. When it came to me, it was missing plate #37,...

Norland has uv activated optical cement although what you trying to do is no mean feat and may well require hashing up some wooden jigs

Maybe part of an epi darkfield set up? Those look like fiber optic channels right?

Mite of some kind. Birds are full of them!