MicrobeHunter.com Microscopy Forum

I would think about this one, the Listed asking price is higher than the above but it is a complete Nikon system, and Make Offer is an option https://www.ebay.de/itm/Mikroskop-Nikon-Labophot-2-Labor-Forschungsmikroskop-Dunkelfeld-Phasenkontrast/264091677870?hash=item3d7d1734ae:g:lwMAAOSwDOpbx3RH:rk:...

Viktor, thanks for the correction, my mistake. So, according to the formula, and the post by Pau in photomacrography: for example: if the camera lens is 50mm and the photo eyepiece is 8X, then 50*8 / 250 = 400/250 = 1.6 relay magnification suitable for APSC sensor. If the eyepiece is 10X though, the...

Perhaps photos under different illumination or magnifications can show whether this is the return-spring of the aileron... amazing image!

It is nice that the stage can be installed either "normally" with the X_Y movement knob near the limb, and roatated by 180 degrees and the knob at front, as in the photo. Less nice IMO that the objectives are not 45 parfocal, it is an older model, and focusing controls are not coaxial.

Yes I agree with 75RR, this does not look like a BH scope but as CHB. From the illumination port and stage, at least.
A BH-2 would be a better choice than these ones, being expandable and using 45mm parfocality objectives. The CHB takes short barrel objectives (ca 36mm).

Viktor, the constructuion you described is impressive. Where does the "frankenscope" name derive from? About the photo setup: In a macrophotography post a couple of years agor, the following formula was shown for afocal setups: (camera lens FL) * (photo eyepiece magnification)/250 = crop factor for ...

About the Labophot, I would inquire the seller : 1. Is the focusing mechanism - both coarse and fine focus controls - in smooth working condition. 2. Is the lamp house in good condition, without mechanical damage and a sound connection to the frame. On the similarly built Optiphot, the mechanical in...

Bees are fantastic little animals. I somehow respect honey bees much more than other insects, like they are little friends, a totally stupid and sentimental approach...

I agree. I was just trying to catch the edge. That objective is an airdale, I will set the BX40 up and try 100x oil. Actually I thought of a lower-mag objective... but, if you plan to use an oil objective, the crystallization must occur between a slide and a coverslip. Is that how the previous crys...

Update: new inexpensive Chinese no-brand 10XWF FN=23 eyepieces, instead of the original Olympus G10X FN=22. (thanks MicroBob for the recommendation). The small extra FOV is not so important - but the new ones have a high eyepoint, about 15-20mm, vs about 10mm highpoint of the old ones. That makes a ...

Perhaps this will fuel the discussion: 2 image stack IX70 60x unstressed POL objective EOS 7D Not so clear I could mail the slide to 75 and let him shoot it in DIC I think that the crystals are fairly thick, say hundreds of microns. Perhaps even the (relatively) deep field of focus of the 60X objec...

Methylene blue is popular for staining mammal cells in cultures.

Very pretty ... but something is worrying me: There seem to be hard edges on the advancing 'front' ... but I would have expected to see a more fractal-like appearance. What we really see is a quite complex process. Crystallization takes time. Evaporation of the solvent takes time as well. If the la...

Just a text line

IMO, India Ink is not a good stain. The pigment inside is tiny soot particles, not molecules, so it is a suspension, not solution. Such particles are expected to coat all solid surfaces, whereas biological stains are meant to stain differentially, and allow distinction between parts of the specimen ...

Something wetter than water should penetrate the fungal mass better. Maybe. Add a small drop of dilute soap, for example a kitchen washing liquid soap, into the water to lower its surface tension by orders of magnitude and thus increase its wetting properties. Stains that are dissolved in alcoholic...

I am assuming that there must be some sort of nifty esoteric explanation. :geek: Like what is being absorbed? The fungus is growing on carrots that were stored in a plastic bag. I kept the fungus growing for further investigation and to attempt a positive Id of the fungus. Sclerotia is a bad omen f...

Where is the fungus in the picture? is it growing on the carrot itself, within the white circle, or is it in the background around the carrot?

I love the Christmas tree and needle images!

I have much less experience with Olympus, than some other brands. What is the S for? With some brands SPlan means semi-plan, or around 80-90% of the field , plan. Vickers , uses the term Microplan for the same thing. I once thought that it was semi-plan indeed... but no, they mean super-plan. Citin...

Selling my Olympus SPLAN 10X/0.30 160/0.17 objective. It is in excellent condition, very clear optics that I double-checked with a stereoscope. One tiny scratch on barrel tip (see photo). Yields a superb, bright and flat field of view. I am selling it because I prefer to stick with Zeiss objectives ...

Let me ask this simple question, overall regrading the illumination issue and focusing issue, how do you find the quality of the images? at least at center with 4X? I am smelling from the posts that i should just dump the microscope then and buy another one? I still have one more image i didn't pos...

The moss shown above grows to form dark green patches. Its neighbor is another type of moss (another species?) of a lighter green tint and narrower leaves. The composite moss carpet is shown in photo no 8 below. I could not find its antheridia or other reproductive organs - perhaps too early in the ...

Sorry, small mistake: Uniform focus for the 40X, slightly less so for 4X image. About the 40X image: do you have a condenser, a condenser aperture iris, a field aperture iris? perhaps the image suffers from flare, too bright light over parts or most of the FOV. To remedy that, one has to create Kohl...

I just copy my comment from the other post "cheap objectives". I have just noticed the brightfield 4X and 40X images. They look quite good to me! In my humblest opinion, I think that the whole field across the image is in the same focus, uniform. This holds for the 40X, slightly less so for 4X image...

rserpell wrote:And, yes Jim, the magnification is close to 10x. I just wonder why no markings to indicate that in the barrel.
Ricardo

The yellow inscribed ring on the barrel means it is an 10X.

Do you have images taken with your 40X objective that show the problem? preferably a brightfield image, not darkfield. Also, it might be useful to validate the planarity of the FOV with a simple high-contrast monochrome pattern, possibly black on white or any other combination of a dark color and br...

... the specimens are prepared to be flat, so that i felt i see it sharp, but when i use camera then this sharpness is gone, it is either center is sharp or the edges, here where it comes about i want Plan objectives so i can have the sample all flat field to edges either by eyes or by camera... Yo...

To really understand the compatibility among the optical components and the advantage of certain components (for example, plan objectives) it helps to read the explanation in microscopy sites, for example, the ones by Nikon, Olympus or Zeiss. Moreover, it helps to choose the upgrade according to wha...

I totally agree with photomicro's recommendation above. 1000Eu is a lot of money (for me at least). But it helps to see examples, so here is one: https://www.ebay.fr/itm/Leitz-Wetzlar-Ortholux-II-Trinocular-Microscope-with-Quintuple-Turret/264099130636?hash=item3d7d88ed0c:g:7rkAAOSww1hastrk:rk:2:pf:...