MicrobeHunter.com Microscopy Forum

Of course it's solvable using the trinocular head. The Canon EOS Utility 3 is not listed on the Canon website as being compatible with the R10. It looks like EOS Utility 3.16.0 is the newest compatible version. Canon support should have known that. There's a newer version of PDP 4 as well. The R10 u...

No. The BX-40 stage mount is larger, so the BH-2 stage won't fit.

A new toy from Nikon: https://www.microscope.healthcare.nikon ... ome-camera

What is your budget for a BH-2 DIC set?

Yeah, I bid a ridiculous amount for the Christmas Tree slide - I think I have all of the others - but it went way past what I thought was remotely possible. Good thing the money went to Klaus' wife!

It's easy. The mountant really isn't important as long as it lasts. I've had to re-coverslip slides I made in the 70's because the mountant dried up and pulled back from the edges of the coverslip. More modern versions work better and last a very long time. You can use Canada Balsam, Permount, Eupar...

The hand-held pipette bulbs are good for larger volumes, but nothing this small and requiring this much control. The blood dilution pipettes use really small volumes of blood and diluent. I've never seen anyone use a bulb or syringe with one of these pipettes. It would be really difficult to do when...

GerryR wrote An article I read on a medical site (?) stated there are large variances between techs performing the same counts using the same samples and Hemacytometer, I'm guessing due to technique. So, that would tell me that certain results would warrant further testing with, perhaps, different m...

Triarch are nice, US made teaching slides that are still being made. Generally hard to beat for quality and range of subjects. Pretty pricey new though: https://www.triarchincorporated.com/

Nice!

The CV's of manual chamber counts are pretty high. There is a lot of variability in any manual process. Consistent mixing of the particles/cells in the liquid, consistent sampling technique, consistent loading volume, accurate dilution, are you counting a single type of particle or cell or are you t...

Looks like Dero digitata.

Nice!!

Yep. Missing binocs mean it will have to be collimated, if you can find a set. Collimation isn't easy. I missed that!

It's not so much a matter of buying cheaper parts, it's current parts. Also, when new versions come out they usually quit manufacturing the older version, even if it's compatible. The few available are old, unsold stock. Try to buy stage racks for a BX-50 from Olympus and you'll see what I mean. Rem...

I just noticed a big problem in the head of the 41. The light path control rod is broken off. :shock: The rod is attached to the prism assembly by a very fine (read fragile) screw thread at the prism end. Obviously this one got banged against something once and broke it off. You can't tell by lookin...

Remember, when the documentation for the BX-50 was written, the U-PCD2 didn't exist, so it's pretty hard to reference a non-existent part. The U-PCD2 is compatible with the BX-50, as are almost all of the other BX series accessories. The stage and condenser mounts are the same and the dovetails are ...

Lets see.. Before you get started, here's really nice video by Peter Evennett explaining how your microscope really works. Watch it. Seems a bit droll in the beginning, but I guarantee you'll learn something: https://www.youtube.com/watch?v=60_jgZtyR6U Now on to the research... From the Foissner lis...

Excellent book. I originally read the pdf as that's what came out first. I jumped at the chance to but the hard cover when it came out. Very well done!

The U-PMTVC is a direct swap for the BH-2 version MTV-3 and works even better as it has the ability to parfocalize. The easy way is something like a Panasonic GH4, or other M4/3 camera, an NFK 3.3x (which with the 0.3x U-PMTVC3 give essentially direct projection with the eyepiece corrections needed)...

Use a reversed 10x wide field objective as a magnifier to check the lens condition prior to and during cleaning. Just Look at light reflections off of the surface of the objective lens to see any contamination or remaining film. 70% to 90% isopropanol and good cotton swabs work fine. I actually use ...

I think he only has one, the upper analyzer.

You need a polarizer above the lamp as you suspected. The U-POT is fine, and what it was designed to use, but they're relatively expensive and a bit hard to find. Get yourself a 45mm linear (not circular) photography polarizer. Anything sized to fit the light port or larger (even a little smaller) w...

I'm about to turn 74. :shock: And I used microscopes professionally, with patient lives dependent upon the results, for over 40 years. Now I do things like this for fun: https://www.nikonsmallworld.com/galleries/2018-small-world-in-motion-competition/stephanoceros-fimbriatus-rotifer-feeding And this...

woyjwjl, First, I was referring to centering the phase annuli with a CT, not DIC. And you'll notice "need" is in quotes. Second, you don't need the CT to see the interference fringes either. Just pull the eyepiece and look down the tube. You can do the same thing with the phase annuli, it's just a l...

@tom jones but if I want to do BF with the BH2-UCD won’t I be hindered by the NA 0.9? Why is it so much lower than even the standard 1.25 abbe condenser. No. Oil. Everything in microscopy is a tradeoff. You give a little one place to get a little somewhere else. You only need enough resolution to a...

Better is probably a bit subjective and depends on what your ultimate goals are. If you want to do phase, the Phase/NIC condenser has you covered, as it has phase annuli as well as the DIC prisms permanently mounted. But you need phase objectives, and these are not optimal for DIC. So, two sets of o...

The condensers are pretty easy to find. The slider and intermediate tubes however are extremely hard to find. I just bought the first set I've seen in more than a year. And the slider needs repair!

Those look like they're from a clinical lab. Gram stains - all under-decolorized too :roll: . If I had to guess, they look like vaginal swabs, or maybe sputum specimens contaminated with a lot of saliva :shock: . Spit, actually. Squamous epithelial cells in several, lots of neutrophils in some, and ...

Here's a paper on University of Calif Riverside's Entomology department's use of DIC and video to create "virtual voucher specimens" for new species of nematodes: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2620590/pdf/296.pdf While it may not show all the features you're looking for, it should sho...