MicrobeHunter.com Microscopy Forum

Plasmid wrote: ↑

Fri Dec 11, 2020 10:02 am

...answer additional questions like which grease to use...

Curious what they recommended?

BramHuntingNematodes wrote: ↑

Sat Dec 12, 2020 12:32 am

nikopol wrote: ↑

Fri Dec 11, 2020 10:18 pm

Plus everyone had that toy microscope in childhood, and knows how painful and useless it is.

Gosh yes amen.

Best experienced Christmas afternoon following months of anticipation:

I still have not tried anything other than doublet+181 so nothing useful to add there. But it seems like a setup with well-corrected telephoto camera lens connected directly to the teaching bridge will give similar lateral CA as direct projection through the normal telelens, since the telelens is re...

Yeah that is the situation in the loose one I have, mentioned earlier in the thread, which is why I could not give a definitive answer on the orientation, only speculation based on the shape of the mirror relative to the light beam.

I guess there is a bit redemption for the manufacturer, that you did eventually managed to talk to someone who understood the problem, but still... wonder if the first step of their tech support procedure to just always tell the customer the behavior is normal and see if they go away?

In case there was any misunderstanding, what I meant was: Before gluing the mirror the first time henryr had not asked how to orient it on the bracket or mentioned any difficulty following Pete's advice to use the original glue marks, so there was not much reason for anyone to have looked carefully ...

These scopes have a confocal illuminator on them (that is factory adjusted per scope) and it appears that the optical mechanism (a two way mirror system) delaminates on the top optical surface and leaves an air bubble between the glass and the mirrored(?) surface. Post about repairing one from a co...

I'm a bit disappointed that the photos in my first post, with the mirror 90 deg.s out of the correct position, didn't get someone to tell me the position was wrong. I'd note and mark where the telltale glue lines are to get it positioned right... Asking then waiting for clarification before gluing ...

Just to clarify first, from your video it looks like the coarse friction adjustment is working fine -- you tightened it and the coarse focus stopped slipping? So the problem is the friction of the fine focus relative to the coarse focus? Partial disassembly, like a member mentioned previously grease...

Until now I used a Nikon Coolpix S6900 attached to one of the ocular, with an aluminium adapter made on the lathe, that allowed me to do acceptable stackings, but some days ago it was gifted to me a mirrorles Olympus E-M10 Mark II. As I'm a total noob both in photography and microscopy, I would ask...

Here is a picture of my M200 taking a picture (trinoc port) in live view - you see the subject, magnified 5x in the live-view screen, is blurred, but the info display on the LCD is crisp and clear. Looks strange. Is that pixelated appearance as if looking through a window screen what the camera liv...

But, I don't think that is the root of the problem because I cannot obtain crisp focus before I take the shot. The M200 gives me a live view on the LCD display, and lets me zoom in 5x and 10x on the image for fine focus. Zooming in that way before I shoot only magnifies an out-of-focus image. If yo...

The way the image looks smeared/doubled in these crop, maybe motion blur due to the shutter? Have you tried electronic shutter modes or varying exposure time? Sort of confusing how the direction and strength of the smearing varies so much across the image, but if not motion, seems like there would h...

Actually under better light I can see a clean ring on the mirror where the glue was and it is reasonably close to centered, definitely not as far offset as you have it in the most recent photos, but I still can't tell how it was originally rotated.

I have one in the same condition as yours. It is difficult to tell the original position as Pete suggests because the glue was applied in a ring and failed very cleanly at the glass interface leaving no residue. However I think it would make more sense for the mirror to be rotated 90 degrees (in the...

Sold in less than 24 hours:
https://www.ebay.com/itm/Reichert-Micro ... 4261645206

Heres a couple of pics ... The head rotated If I am interpreting the photos correctly they appear to show the spot in the same place on the phone with the head at two different angles? If so I think that is a pretty good indication that the spot is somewhere in the head, not in the illumination sys...

In addition to rotating the head as Charles suggested, could also try rotating the nosepiece slightly and see whether the spot moves sideways. I'm with Hobbyist that it's likely in the illumination system and possibly heat-related. ... Yes, the mirror that reflects the light into the condenser. ... ...

Does the spot look the same through both eyepieces?

I'm finding that going much bigger, and especially at 100%, the images get grainy and indistinct so not so good. ... I've been wondering if it might be possible to treat / clean-up the diatoms in situ somehow? I've not come across anything but it would be great if there was a way to do it! Are the ...

Found a schematic, figure 10 shows what the switch does:
Texas Instruments - AN-556 Introduction to Power Supplies

... but could you please explain how voltage selection might be achieved when there are only two wires connected to the switch ? The two large aluminum electrolytic capacitors near the top of the photo would be in series. With the switch open (220-240 V position) the bridge rectifier (just to the l...

Have a look at step (3) here... Looks like the one in the PDF has the same voltage selector switch on the back as the one microb posted. Switch-mode power supplies requiring manual voltage selection were common through at least the 90s. The selector switch is also visible in the internal shot, circ...

If manual selection is necessary (I don't see any obvious sign that it is but am not sure based on the label alone) there may be a little jumper card behind the fuse drawer:

Higher-resolution close-up photos of the label and power entry module (circled in red) may help.

With a distinctive target like a ruler with fine graduations, and with the camera in live view mode zoomed in all the way, maybe you can see the motion in real time relative to fixed guide lines or focus area boxes or whatever on the screen? If so you could try to narrow down where the sensitivity i...

Thanks Bruce. Do you know if ciliates like these actively change cell shape to help fit around obstacles? I know intuition about how stuff moves doesn't scale down well, but after watching them navigate the tight spaces for a while I get the sense they are deforming a lot more than would be expected...

Video is normal speed with gamma adjustment to enhance contrast. 100x objective, 3.5 um diameter, trailing flagellum is 7 um long:

After watching a few more I really think the swirling/tumbling motion is internal. There is no apparent disturbance of stuff surrounding the cell and there appears to be quite a bit of relative among contents at different depths within the shell, inconsistent with the entire cell rotating and the co...

LouiseScot wrote: ↑

Sun Nov 22, 2020 9:21 pm

(Anyone know what the filaments with the moving granules are?)

Possibly fungal?