Search found 729 matches

by microb
Fri Aug 20, 2021 4:21 am
Forum: For forum members who want to buy and sell equipment
Topic: Axiomat Zeiss Parts
Replies: 1
Views: 4646

Re: Axiomat Zeiss Parts

I'd be interested.
by microb
Thu Aug 19, 2021 3:42 am
Forum: Home-made microscope adaptations
Topic: BX stage on BH2 substage
Replies: 10
Views: 7238

Re: BX stage on BH2 substage

Hi, any ideas how to adapt a BX stage (u-svrd-2) to BH2 substage? Thanks Making some corrections to the BH2 substage numbers which I had mentioned needed to be double checked The bearing pad idea mentioned below with the correct BH2 substage diameter would be 8.5mm thick. I have not tried this, but...
by microb
Wed Aug 18, 2021 10:10 pm
Forum: Home-made microscope adaptations
Topic: BX stage on BH2 substage
Replies: 10
Views: 7238

Re: BX stage on BH2 substage

qanunji wrote:
Thu May 06, 2021 8:45 am
Hi,
any ideas how to adapt a BX stage (u-svrd-2) to BH2 substage?
Thanks
I have the BH2 and BX subsages, but the older ones are in storage someplace. What are the dimensions of the BH2 substage again? I think it was smaller in diameter and bit taller than the BX.
by microb
Sun Aug 15, 2021 7:21 pm
Forum: Microscopy accessories
Topic: BX/AX Filter Cube Turret Labels
Replies: 1
Views: 1959

BX/AX Filter Cube Turret Labels

Testing paints on a scuffed steel surface to make some mag tags for a filter cube turret.

Might try a primer coat to get the black to hold better. Even with a sanded surface the black scratches off too easily. Note the green glow under blue light.
by microb
Fri Aug 13, 2021 5:35 pm
Forum: Illumination Techniques
Topic: Positive Phase Contrast Objective Phase Plates
Replies: 53
Views: 18236

Re: Positive Phase Contrast Objective Phase Plates

Oh no this is different from the apodizing experiments of another poster. Here we are trying to assemble a phase plate with phase retarding film set at right angles plus polarizing filter on the light source. Proof of concept worked ok now on to refinement. Sorry. I should have checked. I thought y...
by microb
Fri Aug 13, 2021 3:59 am
Forum: Illumination Techniques
Topic: Positive Phase Contrast Objective Phase Plates
Replies: 53
Views: 18236

Re: Positive Phase Contrast Objective Phase Plates

I think you've been talking about this in another thread. It sounded like you wanted to add rings on the top lens of some non-PH objectives but do the rings in such a way as to have 50% transparency edges to help hide the phase contrast halo issue. I was think of putting the rings on a BK7 window, a...
by microb
Mon Aug 09, 2021 5:15 pm
Forum: Illumination Techniques
Topic: Anyone try one of these LED setups?
Replies: 13
Views: 6871

Re: Anyone try one of these LED setups?

Greg Howald wrote:
Mon Aug 09, 2021 5:59 am
Looks interesting. Makes me wonder how it might work as the top light on my metallurgical scope.
I might try it with some mini-inverted Unitrons I'm going to sell.
by microb
Sun Aug 08, 2021 8:00 pm
Forum: Illumination Techniques
Topic: Anyone try one of these LED setups?
Replies: 13
Views: 6871

Re: Anyone try one of these LED setups?

So I tested the LED lamp on an AX70 using an LGAD adapter -- reflectance only. Have not tried transmittance. It fits in, but needs to go in further to make the aperture and field stops work correctly by properly lining up the conjugate planes. Intensity surprisingly is not an issue. The real issue i...
by microb
Thu Aug 05, 2021 9:41 pm
Forum: Illumination Techniques
Topic: DIC Question
Replies: 257
Views: 3114585

Re: DIC Question

Thorlabs sell DIC prisms but, of course, everything from them is expensive! A Nikon N2 Condenser prism is about £908 with tax and a 20x prism slider is the same. So starter Nomarski DIC parts for £1800.... Tempting. If anyone knows of another source for less, I'd obviously be interested :) Maybe th...
by microb
Wed Aug 04, 2021 9:01 am
Forum: Microscopes and optics
Topic: Phase contrast insert for objective?
Replies: 4
Views: 2276

Re: Phase contrast insert for objective?

Came across this listing for a Leitz EF 10x objective with a "phase contrast insert": https://www.ebay.com/itm/384310270761?epid=1725023138&hash=item597aad5b29:g:0CEAAOSw995hCXFu I don't see how that insert could possibly work for phase contrast. Does anyone know what it really is? For it to be a L...
by microb
Tue Aug 03, 2021 2:18 pm
Forum: Illumination Techniques
Topic: Optics and Polarization Light Source Question
Replies: 2
Views: 2150

Optics and Polarization Light Source Question

Will polarized light run through and properly cross-polarize given a path of lenses like those in an epi that might introduce barrel distortion, and who knows, maybe Olympus distresses the glass when they make their lamp to epi lenses. A BX61 RLAA has a heavily curved lens aimed towards the lamp at ...
by microb
Mon Aug 02, 2021 4:10 pm
Forum: Home-made microscope adaptations
Topic: RMS and SM1 Threads on Fusion 360
Replies: 2
Views: 2674

Re: RMS and SM1 Threads on Fusion 360

The angle was wrong in the file, so I created a separate file for RMS with 55 degrees. <?xml version="1.0" encoding="UTF-8"?> <ThreadType> <Name>Imperial Microscopy threads</Name> <CustomName>RMS Imperial Microscopy Threads</CustomName> <Unit>in</Unit> <Angle>55</Angle> <SortOrder>5</SortOrder> <Ext...
by microb
Mon Aug 02, 2021 10:21 am
Forum: Camera systems and imaging
Topic: Video Editing Software
Replies: 50
Views: 26838

Re: Video Editing Software

Ben Krasnow for his Applied Science channel says he uses a free license of Resolve: https://youtu.be/ihbYtxaEDSk?t=752
by microb
Sun Aug 01, 2021 10:55 pm
Forum: Home-made microscope adaptations
Topic: RMS and SM1 Threads on Fusion 360
Replies: 2
Views: 2674

Re: RMS and SM1 Threads on Fusion 360

Olympus U-P4RE brass adjustable centering mount for an RMS objective, with adjusting pins and rubber plug when a fixed disk is used with no pins.
by microb
Sun Aug 01, 2021 1:09 am
Forum: Home-made microscope adaptations
Topic: RMS and SM1 Threads on Fusion 360
Replies: 2
Views: 2674

RMS and SM1 Threads on Fusion 360

If you need to do threads in Fusion, this posting gives an xml file that is a must and so far appears to be working. https://forums.autodesk.com/t5/fusion-360-design-validate/how-can-i-make-an-internal-rms-royal-microscopy-society-0-8-quot/m-p/8888486/highlight/false https://knowledge.autodesk.com/s...
by microb
Sat Jul 24, 2021 7:21 pm
Forum: Microscopy accessories
Topic: Wired vs Geared Stages
Replies: 6
Views: 3578

Re: Wired vs Geared Stages

For the Olympus BX 'scopes the wired stages I think have no overhang when moved to the limit in the X-axis. This 'overhang' of the rack has caught me out a few times, and I'd really like a wire-operated stage which doesn't have this problem - but it's finding one for my BX50 and of course affording...
by microb
Sat Jul 24, 2021 7:13 pm
Forum: Microscopy accessories
Topic: Wired vs Geared Stages
Replies: 6
Views: 3578

Re: Wired vs Geared Stages

MichaelG. wrote:
Sat Jul 24, 2021 5:06 am
This is a tentative response, Ted … because there is nothing currently pictured in your post

A wire system can be built [at reasonable cost, and without excessive friction] to be completely free of backlash.

MichaelG.
Fixed it. I changed the image format so the website likes it.
by microb
Sat Jul 24, 2021 12:12 am
Forum: Microscopy accessories
Topic: Wired vs Geared Stages
Replies: 6
Views: 3578

Wired vs Geared Stages

What is the advantage of a wired stage like the one pictured here. Apparently some people abhor the older style rack pinion design and only want a stage with a wire mechanism. If the gearing is done right, I don't set that the wire and pullies would be that much different in feel. Thanks, Ted [So th...
by microb
Fri Jul 23, 2021 10:45 am
Forum: Pictures and Videos
Topic: Diatoms in epi DIC
Replies: 18
Views: 4668

Re: Diatoms in epi DIC

What does a Leica z6 with Rottermann look like?
by microb
Fri Jul 23, 2021 2:57 am
Forum: Illumination Techniques
Topic: DIC Question
Replies: 257
Views: 3114585

Re: DIC Question

To control the strain applied and not damage the surface, wouldn’t it be better to cut the poly sheet to have supports with more surface area that a rig can make contact with. For example, instead of press point from a rod, what about spike with these blue surfaces to interface with some metal that ...
by microb
Wed Jul 21, 2021 5:56 am
Forum: Illumination Techniques
Topic: DIC Question
Replies: 257
Views: 3114585

Re: DIC Question

Link is missing... I know I had it there, so I edited it above. What I thought was interesting is that he molded the lens and where the plastic was pushed in there was more stress. So he built into the plastic a level of stress. I wonder if that could be done but I guess the rig with the screws is ...
by microb
Tue Jul 20, 2021 7:42 pm
Forum: Illumination Techniques
Topic: DIC Question
Replies: 257
Views: 3114585

Re: DIC Question

Breaking Taps had a video showing the fringing on a molded acrylic lens, which I think was actually polypropylene:

https://youtu.be/JNXgHbBIRYQ?t=1016

[I know I had added the link, but it disappeared. So added it.]
by microb
Sun Jul 18, 2021 4:07 pm
Forum: Miscellaneous
Topic: How really safe is a laser pointer ? new findings
Replies: 6
Views: 3181

Re: How really safe is a laser pointer ? new findings

Cheap green laser will not have an IR filter. Inside they convert from 808nm to 532nm, and a spectrometer will show both peeks. The IR frequency is especially not eye safe. This video shows how to test without a spectrometer: https://www.youtube.com/watch?v=iR1Ku5dnbH8 So if you are cooking your sam...
by microb
Sat Jul 17, 2021 9:46 pm
Forum: For forum members who want to buy and sell equipment
Topic: AX70 frame forsale for parts
Replies: 1
Views: 1733

AX70 frame forsale for parts

Hi, I have an AX70 frame that I tested objective and filter cube turrets on, plus motored transmission filter. I haven't test sparking lamps just yet. But anyway, the main thing is that the vertical focus knobs are too loose on the right and disconnected on the left. The right side knob does move th...
by microb
Sat Jul 17, 2021 12:14 am
Forum: Camera systems and imaging
Topic: Olympus BX/AX light path to camera diameter
Replies: 1
Views: 1775

Re: Olympus BX/AX light path to camera diameter

<r>So I don't have a DIN retarder plate, but if they are 20mm wide, then the filter has to something down in the 17mm range.<br/> <br/> "Modern first order retardation plates are built in a frame having standardized DIN dimensions (6 × 20 millimeters) that will enable their use in a variety of micro...
by microb
Fri Jul 16, 2021 10:01 pm
Forum: Camera systems and imaging
Topic: Olympus BX/AX light path to camera diameter
Replies: 1
Views: 1775

Olympus BX/AX light path to camera diameter

For the filters like the analyzer and the filter cubes in a BX setup, a 25mm filter diameter is used. Now the AN does a 5 degree slant for the polarizer glass to minimize some reflection, so it's light path diameter is actually elliptical, cut down to just under 24mm. So clearly Olympus didn't reall...
by microb
Thu Jul 15, 2021 6:42 pm
Forum: Illumination Techniques
Topic: Positive Phase Contrast Objective Phase Plates
Replies: 53
Views: 18236

Re: Positive Phase Contrast Objective Phase Plates

This book has a lot of interesting stuff in it. The sample is also assumed to rrtard light by about a quarter wave. I haven't yet managed to understand the arguments in Bennett's book for why this is true and the "Molecular Expressions" primer and related manufacturer-specific sites don't really ex...
by microb
Thu Jul 15, 2021 6:41 pm
Forum: Illumination Techniques
Topic: Positive Phase Contrast Objective Phase Plates
Replies: 53
Views: 18236

Re: Positive Phase Contrast Objective Phase Plates

patta wrote:
Thu Jul 15, 2021 8:36 am
Also posts #6 (book link, pag. 87) and #11 plz
Cool. For some reason I did not see those posts till just now. The forum UI was hiding it from me.

Thanks,
Ted
by microb
Wed Jul 14, 2021 4:42 am
Forum: Microscopes and optics
Topic: Questions about metallurgy on Olympus
Replies: 44
Views: 12549

Re: Questions about metallurgy on Olympus

I wouldn't retrofit this setup. The halogen bulbs are cheap and easy to source and give off good light. My second choice is retrofitting a fiber optic light guide from a 150w source. Retrodiode is good but I'm not sure about the color temperature, or if I'd want to use a pwm-dimmed LED for photogra...