MicrobeHunter.com Microscopy Forum

I would guess that the lower depth of field of the 40x objective caused you to have focusing problems. It might (as a matter of fact) have been in focus, but because of the lower depth of field areas above and below the focus plane might have interfered, and therefore given you a subjective impressi...

Hello, and mounted with transformer varnish what is transformer varnish? Is it the "paint" that holds the coil of a transformer in place? If so, where did you get it and is there any shrinkage when drying? Is it dissolvable in alcohol? I am asking, because I am looking for a cheap, safe and fast dry...

Hello, Hay infusions are sometimes used to culture paramecia and other microorganisms, but the problem is that they also promote rapid bacterial growth. The bacteria (while needed as a food source) may soon overgrow the Paramecia and reduce the oxygen content of the water (causing everything to die ...

Hello and welcome to the forum. It looks like a "test" of a testate amoeba. Tests are the shells. As a matter of fact, it is not only the empty shell, but you can also see the pseudopods extending from it, so it is alive. The pseudopods are the clear jelly-like extensions from the test. Here are som...

I would like to wish all members of the forum and all readers of this forum a Merry Christmas and Season's greetings!

At this point I would also like to welcome all new forum members, who have recently joined the forum.

Oliver

If you want to do much photography, then the convenience (in my opinion) of a trinocular outweighs many other considerations. If you want to capture fast moving organisms, then light plays a much more important role, but if you observe static specimens, then it really does not matter if your exposur...

Other sites suggested that it might also be worth trying to clear your browser cookies (history). This forces the forum to reset the cookies which it needs for timing.

Oliver

Looks similar to a plant trichome: http://waynesword.palomar.edu/images/acucor1b.jpg

Increasing optical zoom increass the amount of incoming light, giving a cleaar image Well, actually increasing the zoom decreases the light, because you are looking at a smaller part of the specimen. The light of this "small part" is then magnified, hence spread out over a larger area. This reduces...

1. Could it be that the focus lock lever of your microscope (if it has one) prevents the full focussing? it can be found next to one of the coarse focus knobs. 2. Some microscopes allow for the adjustment of the focus travel with a screw. this is to prevent the stage from crashing into the objective...

Travis,
The stacking of red blood cells that you refer to is called rouleaux formation. There a Wikipedia article on it:
https://en.wikipedia.org/wiki/Rouleaux
Oliver.

Hello,
You have to contact the respective company directly. For Leica, visit
http://www.leica-microsystems.com/home/
and click the contact link in the menu.
Oliver

The tool with the knobs is called a micromanipulator. It is used to remove the nucelus of cells and can be used to inject sperm cells into egg cells when doing In-Vitro fertilization (ivf). Dissecting a Paramecium does not result in more information. The contents is fluid and therefore any disturban...

Dear forum members,
I am collecting articles and images again for the upcoming issue of the magazine. All contributions are welcome!
Greetings, Oliver.

Looks like a scale from a butterfly, especially the last two pictures.
https://en.m.wikipedia.org/wiki/Scale_( ... n_anatomy)

Oliver.

If I have a question can I ask you? The only problem, this camera is never constant even with exactly the same settings. 1. You have to set the camera to manual and use the same exposure time and sensitivity. You make one test picture, remember the settings and then use them again. 2. When using st...

Welcome! In the My Microscope section, you can post a picture of your microscope.
Oliver.

I also have this book, used it for university study, it was one of the standard textbooks that I used. It is mostly about bacteria, microbiological methods (bacterial culture etc), chemical structure, and such. It is not specifically about microscopy, but gives a good basic university level introduc...

Hello,
Check the voltage directly at the two "feet" (wires) of the bulb, with the bulb still connected. Maybe there is a problem with the socket to bulb connection due to corrosion. The picture shows that the bulb wires seem to be discolored at the bottom, which could indicate corrosion.

Oliver.

Hello,
I found a comprehensive guide to cleaning the microscope here (from Zeiss):
http://www.well.ox.ac.uk/_asset/file/th ... oscope.pdf

Oliver

Hello, be careful when using xylene /xylol for cleaning. These solvents might dissolve the kit which glues the lenses into place in the objective. You should use a highly volatile solvent (30 ethanol:70 ether mixture), which evaporates quickly and one which can not seep much behind the lenses. This ...

Hello, I really don't want to use a usb camera...already have >1000 invested in my dslr which is 18megapixels...pretty sure it will blow amscope 3mp camera out of water (but again, I'm noob and don't know for sure)... Yes, the quality might indeed be better, not only because of the camera resolution...

Hello, The microscopes in your link are all somewhat different and they can not be compared directly. You first have to define your preferences and "needs", but I admit that this is difficult to do if you intend to use them for hobby purposes. Ultimately it boils down to the simple formula: The best...

Hello, High NA objectives often do not work well for darkfield, but maybe the iris can compensate this (link: http://www.olympus-ims.com/en/microscopes/faq/#15). UV objectives are made of a material which allows UV light to go through (sample illuminated with UV from top, with the UV light passed th...

In response to Gekko's post concerning the size of the LED and the tungsten filament, I wonder if it is possible to use a diffuser above the LED. I once experimentally tried to use LEDs but the illumination was very uneven. You might also want do read the article "Do-it-yourself Microscope LED Illum...

Hello and welcome to the forum. Depending on your experience, you might also consider getting a relatively low cost (but decent) no-name microscope first, to keep the initial investment low. You can always spend more later, when you have more experience. Phase contrast can become expensive quite qui...

The last picture looks like the spirals formed by xylem tissue in plants (for water transport and for strengthening support).
Here is a link with a picture: http://sydney.edu.au/science/biology/le ... 2AB6.shtml

Oliver

Hello, The camera in the link (with the 0.5x reduction lens) can be inserted directly instead of an eyepiece. Try the following: 1. remove the mtv3/cmount from the BH2 trinocular head and insert a regular eyepiece instead. This should work, provided that the tube of the trinocular head has the same ...

Hello,

Just put the identifier into the tags, and not the whole link:

Try this:
[youtube]hsACOUG1z8M[/youtube]

Not this:
[youtube]https://www.youtube.com/watch?v=hsACOUG1z8M[/youtube]

And *uncheck* the "Disable BBCode" option.

Oliver.

1. copy the link to the picture into the clipboard.
2. paste it into the post
3. add the [img][/img] tags arround the url

Oliver.