MicrobeHunter.com Microscopy Forum

So I prepped a slide, placed it on the stage, and turned on the supply for my nifty home brewed LED system for the first time since last spring and... POP!
The LED blew.

Sorry to hear that. Hope you are up and running soon. Any idea as to why that happened?

16x, 95µm, Oblique, Olympus E-p2

Testate amoeba at home and active, which was nice.
Surrounded by what I think might be Synechocystis.

Those are nice images.

As usual, I've got everything against me.

I am going to translate that as: I find it an exciting and stimulating challenge. ;)

Nice detail on the last one. Shows up the granules on the outer border lip!

Wonderful images. A treat as always.

These are very good images, but I have to say that the second one is great.
One can almost feel the shape as it curves towards the center.
Is the center area thinner i.e. single cell thickness or is it the result of image choice for stacking?

Hi DaveH, I bought the E-p2 second hand - body only. I was using the afocal method with legacy OM Olympus lenses at one time, mostly a 50mm with a Micro 4/3 to OM adapter. For a little while now I have dispensed with the lenses and am using the projection method using a KPL 10x eyepiece. See links f...

Here is a link to an interesting and relevant article by David Walker at Microscopy UK. It has been posted before in another thread so some of you may have already seen it. Worth another look. http://www.microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopy-uk.org.uk/mag/artsep08/dw-pa-test.h...

... unless you know about Lyme then you won't know how difficult it is to get an nhs diagnosis to agree that that is what one has.

Yes, we have heard!

Much better! Did your " I think my life changed tonight. " moment include: there is a time and place for fun filters? Repeating the "How to clean your Microscope pamphlet" link. Covers what, i.e. eyepieces, objectives, camera lenses etc and how. http://microscopy.duke.edu/downloads/The%20Clean%20Mic...

I just used black center stops that are slightly too small for true darkfield. Should that not be rings? I was wondering if one could make a home made Phase kit. Apparently one can. See link: http://www.microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopy-uk.org.uk/mag/artsep04/dwphase2.htm...

Agree stills would be easier to compare. Still think Pleurosigma is a good choice as it is not just about resolving the punctae but about how well one can resolve them. Adding UGF + UGF with Pol is a good idea, as it would allow those who do not have Phase, and hence are not able to try COL (Circula...

Hi zzffnn, interesting idea. Would like to test this but was wondering if using Pleurosigma angulatum, also from Kemp's 8 form slide, rather than Frustulia rhomboides might be better. The punctae of Pleurosigma angulatum can be distinguished with a 40x/0,65 objective which would be more representati...

I just use my mobile phone through the lens on my Watson and barnit system 70 microscope

Are the images close to what you see through the eyepiece in sharpness, colour and size?

I find it a little more finicky, seems more subject sensitive than UGF, yet it is promising. Just need to get the hang of it.

Thanks JimT

COL = Circular Oblique Lighting. It is Oblique obtained by misusing a Phase condenser ;)

http://www.microscopy-uk.org.uk/mag/artdec02/pjcol.html

Good catch. Always nice to find one.

Xylem stands out nicely. Looks like neon in the last one. Nice!

Thanks gekko. Thought the COL would come out better. Think you are right on the limitations of Phase "on the thick, multilayered structures"

I believe I have lymes disease and have been very sick for 6 months.

Do not try to self diagnose. Even doctors visit doctors.

25x * 1.6, 75µm, Oblique, COL and Phase, Olympus E-p2



plus another video of the same rotifer:

Was looking at sellers site having followed the link and found what looks like the stand the DIC Condenser was on. See stain marks on dial. Thought those interested in it might find this info useful.

http://www.ebay.com/itm/CARL-ZEISS-BINO ... 35f542fd64

It had a split pillar...

Now that you mention it it kind of leaps out at one! Sorry for the misleading link.

DDE?
http://www.microscopy-uk.org.uk/mag/ind ... c-dde.html

If I have a choice should I stick with the mfr's brand? That would be a yes. While I have heard it is possible to achieve decent results mixing and matching, I would leave that to the compulsive tinkerers; for a first-rate viewing experience, stick to the eyepieces that were designed for the object...

I also immersed the 40 in glycerin for maybe 10 seconds, while the stage was mal-set. Cleaned it immediately, but, well...

What did you clean it with?

See link: http://microscopy.duke.edu/downloads/Th ... scsope.pdf

I was hoping you would post some comparison photographs. The jump from 10x to 40x is quite large, depth of field is much much smaller with a 40x objective. Lighting, focusing and contrast requirements are much higher. Perhaps you should look into getting a 20x objective - it is to my mind one of the...

You might want to do a comparison between your 40x objective and your objective that is nearest to it in magnification. Ideally you would use the same subject for both, something less amorphous would help, a diatom perhaps - do not move it, just rotate the other objective in - and use either Brightf...

Hello, what microscope do you have and what objectives do you use for these 2 pictures?, looks very clear.

Hi CCMOLA, I used a Zeiss Neofluar 6.3x and 25x. Microscope is a Zeiss Standard RA.

See link: viewtopic.php?f=24&t=70

Decided to see if the yellow globules that have been provisionally identified as triacylglycerols were only visible if the weight of the coverslip caused a rupture of the Ostracod's shell. Leading to ...often small oil droplets coalesce to larger drops... following a squish . See charlie g's comment...