MicrobeHunter.com Microscopy Forum

stjepo wrote: ↑

Fri Jan 19, 2024 2:28 pm

Any advice from you will help me.

Sorry and excuse my ignorance what is 'sa and ca'

Regards

Steve

Spherical aberrations and chromatic aberrations, respectively.

Just thought I would dig up this old gem of a thread to convey the story of my success in restoring the opaque black paint on my Zeiss Ultra condenser. The product I used was a rattle can of VHT wheel paint . I didn't buy it specifically for this purpose - I shamelessly salvaged it, full, from a ro...

Maybe there is no problem with the condenser - but the objective turret (nosepiece) is slightly off-position ?

In a complete Kohler setup, there is one more degree of freedom - the distance between the lamp and the collector optics. On my very old microscope for example, which dates from the 1950's, that distance can be adjusted by sliding the lamp housing slightly in or out, until the filament image is def...

In a complete Kohler setup, there is one more degree of freedom - the distance between the lamp and the collector optics. On my very old microscope for example, which dates from the 1950's, that distance can be adjusted by sliding the lamp housing slightly in or out, until the filament image is defo...

Oh, to clarify: this is the typical information posted by sellers about their microscope cameras, which is where I could use some guidance picking out the 3-4 important details to compare among several different camera offerings. HAYEAR USB3.0 Digital Microscope Camera 8 Mega Pixels USB Live Video ...

The same footnote #2 applies to the two dry planapo objectives, 40X0.95 and 63X0.95, on the previous page of that same Leitz catalog (p. 19, top table).

What is the FN of the microscope ? a camera image width of 0.74mm from a 40X objective seems to me very large. If the FN for example is 26, the image diameter through the eyepieces would be 0.65mm. Or is the 0.74mm obtained from a 10X objective ?

How about both bulbs are burnt out, normal end of life ?

Copied from the site of a firm named Synopsys. It shows the light losses when the tip is tapered to produce a narrower end.
They state that losses are substantial.

A light guide does not "concentrate" light or "funnel" it or focus it, regardless of the geometry of the tip (square cut/frustum). As mentioned, one can axially drill a 5mm LED, insert a 1mm-diameter (say) optical fiber into the bore, attach it with epoxy, and cover the LED by means of a PVC shrink-...

… I think, still, that the OPs project will still offer some interesting results … Most definitely … I’m looking forward to seeing them MichaelG. Gee fellows, I don't know if there will be any interesting results. I am a complete beginner in microscopy and a lot of the stuff you guys talk about go ...

Wow!

Same issue here. Cannot upload photos.

apochronaut wrote: ↑

Wed Dec 27, 2023 4:31 pm

https://www.mcgill.ca/oss/article/envir ... -identical

Thanks for pointing out this fascinating subject!

It might help to know which type of microscope illumination is wished. Both brightfield and fluorescence, at least, benefit from a single die LED source, rather than a multi-LED arrangement. Besides, I wonder about the wavelength combination... For visual observation, green or blue or amber light ar...

I have an old condenser top lens, Olympus achromat aplanat 1.4, in fairly good condition I think. Looks similar to yours and perhaps will fit your condenser. Thread diameter 35mm. Top glass diameter 16.4mm. Glass looks clean, except for two tiny chirps (diameter, say <0.5mm) on the top glass. I beli...

You might try very fine syringe hypodermic needles. They go by gauge number, #33 is the finest I ever met, but #27 (and maybe grades #28-#30 too) are easy to find (on eBay, etc). The tip of a hypodermic needle is actually a tiny blade. Another useful very thin utensil, though not a blade, is the nee...

There is a manual named:
Nikon OPTIPHOT BODY LABOPHOT REPAIR MANUAL
for the beginner and experienced technician alike (seriously!)
It has been downloadable from the University of Arizona site.
It provides exploded views and instructions for disassembly of the focusing gear.

iPeace wrote: ↑

Wed Dec 06, 2023 6:53 am

The lens closest to the eye is a single lens. The lens furthest from the eye is a doublet.

Is it a KPL or KPL-W eyepiece ? your description of the issue does indeed indicate delamination.

I can't get any light to come through.... I noticed the back of the cube is labeled "Omega XF06." This describes the following setup: Exciter: XF1005 (365WB50) Dichroic: XF2001 (400DCLP) Emitter: XF3002 (450DF65) as described here: http://www.glenspectra.co.uk/glen/filters/pdfs/XF06.pdf But the Oly...

Perhaps I am utterly stupid but could it be that there are actually two glass filters, in two separate metal holders in series ? One holder contains nice violet-reflecting glass, probably the BP545 filter. It passes green and reflects violet. The other holder contains what looks like a green-reflec...

Perhaps I am utterly stupid but could it be that there are actually two glass filters, in two separate metal holders in series ? One holder contains nice violet-reflecting glass, probably the BP545 filter. It passes green and reflects violet. The other holder contains what looks like a green-reflect...

I am not on track with uploading images, but I think this link will allow you to see pictures of the Olympus BH2 G cube, and its opaque inner surface of the BP545 excitation filtre. It is blocking absolutely all light projected to it. The dichroic mirror and emission filtre seem good. https://photo...

Likely, full control of the camera is only available when it is connected to the HDMI. I wonder about untold limitations in the declared simultaneity of output to HDMI and USB. Also, USB 2 is inadequate for decent videos. Also, the pushbuttons are small and dense and are possibly a source of vibrati...

Thanks to everyone who has contributed to my many questions about epifluorescense. Being new to this modality, new issues keep coming up. One pf my filtre cubes has an excitation filtre that has accumulated something on the internal surface, to a degree where it is completely opaque, and no light i...

I can do blue excitation light. But i would like to see the red autoflorescence of vegetal stuff. I use a RGB LED torch that works wonderfully but i have two diferent position for the cube filter and i think that none of them allows for that. Should i try to change the filters in the cube? I think ...

Perhaps a more or less uniform layer of small opaque particles of a defined diameter can serve for that purpose. Polystyrene spheres and other polymer particles are available from lab suppliers as suspensions that can be diluted with water as needed. A dried drop on the coverslip will make the test ...

Also note that the listed camera lacks a 0.5X reducing lens, which likely will provide a much wider FOV than without it, and that the FPS is relatively low at high quality video. I would prefer USB 3 rather than USB 2, if choosing an eyepiece camera. The listed camera appears to be bundled with a co...

2) I understand what you write - but why is it in the analyzer slider? Any particular reason? What's the benefit of this, in lieu of placing it in the cube itself? Are an analyzer and cube installed together, such that the rays pass through both of them in series ? in that case, perhaps the previou...