MicrobeHunter.com Microscopy Forum

We collected tree base mosses just as we had on our succesful bear hunt (tardigrades) a few weeks ago. The samples are now at my bench with a drizzle of water...I plan to observe for bears before the Super Bowl. Before I transfered the moss sample from the last bear hunt, I tried a DF scan of a slid...

Hi all...yes it was in balmy 40's during the day for almost a week, so good doggies and I went on a woodland hunt for bears(tardigrades).

I look forward to your trials with this COL contrast method,gekko. I now see (pun) that when all illumination is from beneath the specimen..it's all: 'conscopic illumination'? When illumination comes from above the specimen...it's 'episcopic illumination'. So you can have oblique illumination by my ...

Welcome,Duncan! charlie guevara, finger lakes/US

Three (3) charlies in a row...this thread is chuck full! charlie guevara

Hi...land,sea,air...we need to think of communities sorted out with:'microhabitats'...or nitches (?sp?). Though many organisms 'seem' ubiquitous...there are predictable 'micro habitats' within which one expects groupings of organisms. So dropping down the rabit hole to microbes...these sort out in g...

Welcome,Ray! Err...panthers or bronco's? charlie guevara,finger lakes/US

Hi all. Say gekko...is it that DF is a specific and crisp optical plane of a condensers illumination cone hitting objects? And is COL a softer range of the condensers illumination cone hitting objects, rather than COL occurring at a crisp optical plane? When I mix my Labophot condensers phase discs ...

Beautiful,gekko! Is that jet black background 'image processed'..or do your pol filters when CX/POL give that perfect extinction?!

Are your pol filters DIY, or are they a microscope firm set of pol filters, gekko?

Quite an image, love the green glows. charlie guevara

Hi...I am not an expert...but: that photo tube has the specific specs on it for use wither the 12X ocular nested inside it... or the 10Xocular (plus a 5 mm spcer ring to have it parfocal with the 12Xocular). So it performs as a camera adapter with a nested occular. Folks with more knowledge on this ...

Good video clip...but sad demise...I sense it was a gentle cover slip crunch from objective..as the objective 'seeks focus'...right at the git-go there is out of focus first damage of the cell body...and that spinning easily could be osmotic fluid currents as this bleak punctured protist 'disembowls...

Great work,John B...and JimT! Please consider the low cost 'Lunar polarizer filters'...very quality filters, low cost...already paired together in metal mount rings...yet they come apart so you have your polarizer and analyzer filters to place at the different locations in your light train. any onli...

The magnification listed is the objective magnification. 100X was 100X oil immersion. The DF images were taken straight to sensor from the objective with just the telon lens in the head as an intermediary. Diastar 420 microscope. The one marked 10X( the last one) was with a 100X phase annulus and a...

Love your 'porcupine' ,Phil. Years ago I chased one down a rural shale road with a huge landing fish net.( a mamalian porcupine in Chenango Co,/NY)..then as it lumbered ahead of me I thought...naw...hard to pet. But when our son was a child...we enjoyed for years, european hedge hogs as dear pets! S...

I experience a totally similar 'glare obstruction' in my crude attempts to image capture simple 'CX/Pol' images of jemstone inclusions of stream bed clays...visually the color patterns are stunning...but for my image captures...oh so many colors are 'drowned out' by brilliance of the microcrystals o...

Congrats, zzffnn, on an enchanting image to my eyes. What you imaged captured is the pellicle of a deceased Phacus species. This pellicle is quite diagnostic for type of flagellate..just as the crystaline jewel-box frustules of dead diatoms are diagnostic. This pellicle has value to many...just as t...

Thank you, zzffnn, and gecko! My Labophot turrent condenser is:1.25...it is that DF turrent setting which gave that image, ah..as gecko advises..I should go back and look at this diatom slide and take note of the background color visually...and then compare to the image capture background color. But...

To zzffnn's comments, I would guess that using the Plezy, even if it doesn't give you the exact tube length correction, will give a better approximation than an optic-less adapter (just a guess). Charlie, for what it is worth, I assume that your condenser's NA is greater than one, then yes, you can...

Regarding what immersion for condenser: In some cases, you need oil instead of water. For example, if you are doing darkfield or oblique with a condenser with maximum NA of 1.4 or above. If you are using an oil Abbé condenser with NA 1.25 for brightfield, then oil would not do much more than water ...

Charlie, I heard Leitz Plezy was designed for adapting 37mm parfocal objectives to 45mm parfocal objectives. Some did use Plezy to adapt LOMO WIs to DIN scope and considered it fine. Optically though, it is not an exact fit. I would guess (though I could be totally wrong) that tube length addition ...

So with these two coupled adaptors...my: LOMO BF 70X apo, and my LOMO DF and Phase 70X apo work on my Nikon Labophot stand. I have not yet ciphered how to achieve the 'phase contrast' contrast method with this spec'ed objective. Again....1) what type of water for the objective bridge? Fan is a fan o...

Thanks for the comments...clearly in forum when I 'whined' how I jumped at purchase of three LOMO apo's as the cost was so reasonable...forum opine in 2010 was: 'Plezy adaptors will permit these for use in a DIN stand'...dugghh...so I could not locate Plezy adaptors until 2015 ! charlie guevara

Thanks for the thoughts...now...do I 'water bridge' between condenser and slide undersurface to achieve the LOMO spec of NA 1.23? As oil bridge is more 'controlable'...would oil-bridge between condenser and slide undersurface affect performance of these low cost LOMO apo's...with water immersion of ...

Thanks for any commets...what are thes two silver metal adaptors? Quit a few years ago in great microscopy forum: "Microscope"...I was advised that the: Plezy adaptor alone would permit these RMS 'short barrel objectives ' to function on my DIN (45 mm spec objective lens barrel Nikon Labophot stand....

Hi, of course I hand held each of these pleasant silver metal adaptors over fine print on paper...no obvious 'lens affects' noted in the view of fine print through these adaptors...thanks for your comments...thanks for you forum sharing. charlie guevara

please advise or comment on what these two similar objective adaptors are used for in 'best practice'? thanks, charlie guevara

Hi all, thanks to wonderful microscopists in forum shareing use of LOMO apo/water immersion objectives in recent forum threads...I again tried to couple my two LOMO apo/WI objectives to my 1990's DIN (45 mm objective lens barrel) 160 mmTL Nikon Labophot stand I achieved use of both my LOMO apo WI ob...

Yes, thanks for sharing that RAF vendor offering the LOMO apo 70X,1.23 WI dark field and phase objective and the $145 USD sounds terrific price to me. Thanks, Fan. My 2010 Chicago seller gave the entire print out of this objectives specs..and Vlad listed in the specs: 'dark field and phase contrast'...

Yes, yes...bottled water for use with these LOMO apo WI objectives. Err...this is einmams thread, Fan...however...I clearly stated I have a: LOMO 70X,1.23,WI objective speced as: "Dark field and Phase"...I clearly stated that with a Plezy adaptor+ another Leitz Wetzlar adaptor...this:'DF and Phase o...

Sweet combo of LOMO/WI objectives,einman! Will you put much worry into the actual water you use for the water immersion bridge? I finally made my LOMO 70X 1.23 DF and Phase work with DF stop on my Nikon Labophot! It took a combo of a Leitz Wetzlar Germany Plezy+ a Leitz Wetzlar Germany adaptor which...