How to prepare and preserve tardigrades on a slide

[shawn perez]

Can someone help me prepare a permanent slide of a tardigrade at home the easy way? So i can observe it further

[deBult]

Would appreciate some guidance as well.

[wporter]

Our study showed that among the nine mounting methods tested, thermal inactivation of animals at 60 °C for 30 min followed by briefly pressing the coverslip with an entomological pin produced specimens fixed optimally for morphometry and imaging (i.e. maximally stretched and levelled but not deformed).

From the abstract of the following paper:

https://www.researchgate.net/publicatio ... microscopy

[deBult]

Tnx, requested the paper.

Thermal inactivation of 30 mins at 60 centigrade would suffice.... and they send them to the moon and expect them to reincarnate when returned to earth???

[Bemoc]

LMAO deBult. You're funny.

[MichaelG.]

Thermal relaxation by incubating live individuals at 60 °C for 30 min, i.e. until dead and fully straightened, and then mounting them on slides ...

For me, at least, this gives a whole new meaning to the word ‘incubating’

MichaelG.

[Bemoc]

And I'm still kicking myself for losing my entomological cover-slip poking pin.

[shawn perez]

Our study showed that among the nine mounting methods tested, thermal inactivation of animals at 60 °C for 30 min followed by briefly pressing the coverslip with an entomological pin produced specimens fixed optimally for morphometry and imaging (i.e. maximally stretched and levelled but not deformed).

From the abstract of the following paper:

https://www.researchgate.net/publicatio ... microscopy

Thank you i have requested full text

[mrsonchus]

Interesting. I think this is the full pdf downloadable...

The chloral hydrate puts a stop on the Hoyer's formula here in the U.K. that's for sure - an alternative must be out-there though?

[shawn perez]

Interesting. I think this is the full pdf downloadable...

The chloral hydrate puts a stop on the Hoyer's formula here in the U.K. that's for sure - an alternative must be out-there though?

Hey the link doesn't work can you maybe share it again?

[Element 56]

Interesting. I think this is the full pdf downloadable...

The chloral hydrate puts a stop on the Hoyer's formula here in the U.K. that's for sure - an alternative must be out-there though?

Is any amount or any product containing any amount of chloral hydrate illegal? Can you order ready made Hoyer's? I use Hempsteads Hoyer's and I really like it for semi-permanent mounts. It's vert easy to use and inexpensive but of course it contains Chloral Anhydrous.

http://www.hempsteadhalide.com/

Kirby

[Mraster2]

Interesting. I think this is the full pdf downloadable...

The chloral hydrate puts a stop on the Hoyer's formula here in the U.K. that's for sure - an alternative must be out-there though?

Hey the link doesn't work can you maybe share it again?

Lets see if these links work,,,

Published in :
Zoological Journal of the Linnean Society, Volume 178, Issue 4, December 2016, Pages 785–793
https://academic.oup.com/zoolinnean/art ... 85/2691469
If you have a slow internet that link will be slower to load than usual for a web page, cos it contains the whole text as well, below the abstract, so save the page as an html-complete for offline browser use.)

link on that page to the PDF :
https://academic.oup.com/zoolinnean/art ... j12457.pdf

(edit) ,,, yep, looks good with me.

[deBult]

Tnx: this link works (never received any answer when requesting the paper).

[shawn perez]

Interesting. I think this is the full pdf downloadable...

The chloral hydrate puts a stop on the Hoyer's formula here in the U.K. that's for sure - an alternative must be out-there though?

Hey the link doesn't work can you maybe share it again?

Lets see if these links work,,,

Published in :
Zoological Journal of the Linnean Society, Volume 178, Issue 4, December 2016, Pages 785–793
https://academic.oup.com/zoolinnean/art ... 85/2691469
If you have a slow internet that link will be slower to load than usual for a web page, cos it contains the whole text as well, below the abstract, so save the page as an html-complete for offline browser use.)

link on that page to the PDF :
https://academic.oup.com/zoolinnean/art ... j12457.pdf

(edit) ,,, yep, looks good with me.

Thank you

[mrsonchus]

Apologies for the link, thanks for finding a good one Shawn.