Good Prepared Slides as examples of water life AND pros and cons of concave slides?

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cpsTN
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Good Prepared Slides as examples of water life AND pros and cons of concave slides?

#1 Post by cpsTN » Sun Feb 21, 2016 1:32 am

To assist in my identification of microscopic water life, I thought I would buy a sample box of several prepared slides, say 50 or fewer, to show me what the common organisms look like in real life - well, without the water I guess. To begin, I have been looking at several offerings on Amazon. Before I buy, if I do, I thought I would ask what others here have bought or have done to help in matters like these. Also, does anyone here use concave slides? Pros and Cons of these?
Charles Sands
Murfreesboro, TN 37129

MICROSCOPES:
AO 110
...objectives, infinity:
10x plan #1021
45x achro #1116
50x plan, oil iris #1016
100x plan, oil #1024

Amscope SE305, Stereo
...objectives: 1x, 3x
...EPs: 5x, 10x, 15x

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Re: Good Prepared Slides as examples of water life AND pros and cons of concave slides?

#2 Post by charlie g » Sun Feb 21, 2016 2:39 am

Hi, 'depresion well slides' or 'concave depression slides' are often handy for water fleas, hydras, a tuft of algae which has complex structures, water mites, flat worms...and the eggs of so many wee creatures...they work well (pun) either single well, or two wells per slide. Of course many will place their objects of water life on a coverslip...and carefully plop this over the well...so the droplet with life is inverted over the well.

Prepared slides are so dense with information (as John B. often shows us all)...it's a great path itself besides live specimen microscopy. You must be careful not to catch the prepared slide collecting fever.

A Stefano in Italy runs an eBay store with wonderful prepared slides, as does Klause Kemp who I don't think uses eBay.

all the best, charlie guevara

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Re: Good Prepared Slides as examples of water life AND pros and cons of concave slides?

#3 Post by DaveH » Sun Feb 21, 2016 11:09 am

Hi I use well slides for looking at water from the bird bath, they are a quick simple way of looking at water, everyone should have 1 or 2 or maybe more.
The down side is both the size and thickness of the sample, the faster moving life can be hard to follow with all but the 4x and at high powers the depth of field is so shallow you need to keep both hand busy with focus and slide movement, but having said that I still use well slides the most.

Dave

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Dale
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Re: Good Prepared Slides as examples of water life AND pros and cons of concave slides?

#4 Post by Dale » Sun Feb 21, 2016 4:30 pm

I despise these. They charge too much for dirty plastic slides. If there are good ones, that would be different.
Above rant is for prepared slides.
Using a well slide is like scuba diving, you go left, you go right, and you go up and down!
Must be careful to not crunch longer objectives.
With a flat-slide your specimens will fry, with a well-slide they will boil. (was that bad?)
Dale
B&L Stereozoom 4. Nikon E600. AO Biostar 1820.

JimT
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Re: Good Prepared Slides as examples of water life AND pros and cons of concave slides?

#5 Post by JimT » Sun Feb 21, 2016 6:25 pm

My only experience with prepared slides was a set from Amscope which I promptly returned. They were awful!

I use a depression slide when I expect to see larger critters; ostracods, Cyclopses, etc to avoid crushing them. My scope has an LED light so no worries about cooking them.

JimT

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Re: Good Prepared Slides as examples of water life AND pros and cons of concave slides?

#6 Post by einman » Sun Feb 21, 2016 10:01 pm

I have been on Klaus Kemp's site and even tried to contact him to purchase some slides but he has yet to respond! Is there a secret hand shake or something?

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75RR
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Re: Good Prepared Slides as examples of water life AND pros and cons of concave slides?

#7 Post by 75RR » Sun Feb 21, 2016 10:17 pm

I find he always responds to emails at this address: Klaus Kemp <klaus.kemp@talktalk.net>
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

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Re: Good Prepared Slides as examples of water life AND pros and cons of concave slides?

#8 Post by zzffnn » Sun Feb 21, 2016 10:36 pm

Klaus also travels to collect diatoms around the world. The old man may not be quick to respond you, but wait a while and remind him if necessary. He will reply you. I have emailed him twice to that talktalk email and he replied within 3 days at both times.
Selling my Canon FD 200mm F/2.8 lens

cpsTN
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Re: Good Prepared Slides as examples of water life AND pros and cons of concave slides?

#9 Post by cpsTN » Wed Feb 24, 2016 5:41 am

[quote="JimT"]My only experience with prepared slides was a set from Amscope which I promptly returned. They were awful!

What was awful about them, Jim?
Charles Sands
Murfreesboro, TN 37129

MICROSCOPES:
AO 110
...objectives, infinity:
10x plan #1021
45x achro #1116
50x plan, oil iris #1016
100x plan, oil #1024

Amscope SE305, Stereo
...objectives: 1x, 3x
...EPs: 5x, 10x, 15x

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Re: Good Prepared Slides as examples of water life AND pros and cons of concave slides?

#10 Post by zzffnn » Wed Feb 24, 2016 2:28 pm

I have about 70 prepared slides (7 boxes) and only like about half of them.

Many pond creature slides are poorly made. Few critters on the slide are intact (not crashed) or stained well. Some slides barely have anything on it at all. Some are too thick for high magnification.

Many plant slides are of decent quality though.

I don't have any (big) animal histology prepared slides.
Selling my Canon FD 200mm F/2.8 lens

JimT
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Re: Good Prepared Slides as examples of water life AND pros and cons of concave slides?

#11 Post by JimT » Wed Feb 24, 2016 4:06 pm

What was awful about them, Jim?
Dirty and looked like they were prepared by "preschoolers". Specimens were poorly mounted, often misshapen and damaged, and contained debris.

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charlie
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Re: Good Prepared Slides as examples of water life AND pros and cons of concave slides?

#12 Post by charlie » Wed Feb 24, 2016 4:17 pm

charlie g wrote: Of course many will place their objects of water life on a coverslip...and carefully plop this over the well...so the droplet with life is inverted over the well.. A Stefano in Italy runs an eBay store with wonderful prepared slides, as does Klause Kemp who I don't think uses eBay.
That is a trick I never would have thought of! Thanks Charlie I assume it makes it easier to find your subjects?

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Re: Good Prepared Slides as examples of water life AND pros and cons of concave slides?

#13 Post by charlie g » Thu Feb 25, 2016 3:08 am

Hi, I actually am not a fan of: 'hanging drop mounts'. I see no need for them. Great question...why a 'hanging drop prep?'!

When I have a drop of pond water on a clean slide..the convexity of the water globule..makes DF, or phase effects function only in specific zones of the specimen droplet with 2X,4X,10X,or 20X objectives...maybee the surface being flat at top face to the objective is good?

If it's just because a coverslip laying as a closed container...to slow evaporation...then that's silly...just keep feeding water to a droplet your observing...so I have no idea why: 'hanging drop mounts' are still used.

If it's for long term observations of a wonderful specimen...forget it...just keep a wetmount slide in a 'moisture chamber' when your not at the bench. charlie guevara

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charlie
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Re: Good Prepared Slides as examples of water life AND pros and cons of concave slides?

#14 Post by charlie » Thu Feb 25, 2016 5:56 pm

zzffnn wrote:I have about 70 prepared slides (7 boxes) and only like about half of them.
Many pond creature slides are poorly made. Few critters on the slide are intact (not crashed) or stained well. Some slides barely have anything on it at all. Some are too thick for high magnification.
Many plant slides are of decent quality though.
I don't have any (big) animal histology prepared slides.
I plan to buy a Klaus Kemp test slide that you and others have mentioned Fan.

Since shipping likely will be significant factor, are there other "must haves" to get at the same time?

http://www.diatoms.co.uk/

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Re: Good Prepared Slides as examples of water life AND pros and cons of concave slides?

#15 Post by zzffnn » Thu Feb 25, 2016 6:35 pm

Charlie,

I suggest getting 2 test slides at one time, if you want to save on shipping. In my early years, I have damaged 3 diatom slides by over-heating them with halogen lights. Some mounted diatoms, especially the longer ones, got shifted around and broken into pieces.
Selling my Canon FD 200mm F/2.8 lens

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Re: Good Prepared Slides as examples of water life AND pros and cons of concave slides?

#16 Post by charlie g » Thu Feb 25, 2016 7:07 pm

Hi, zzffnn...how did you 'over heat' the diatom slides? I don't understand. charlie guevara

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Re: Good Prepared Slides as examples of water life AND pros and cons of concave slides?

#17 Post by billben74 » Thu Feb 25, 2016 10:03 pm

I also got some amscope slides. I also found the volvox, paramecium etc. to be rubbish.

There are some good ones though, the insects for example are pretty good. Really nice bee parts.

But I think there are better places to get slides.

I got a few from Brunel (UK) which were all of high standard. I also have a 30 form diatoms from Klaus Kemp.
This is fantastic. I got a strew slide as well which turned out to be less of my cup of tea.

I will get a pair of his (Klaus) test slides soon (thanks for the tip zzffnn).

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Re: Good Prepared Slides as examples of water life AND pros and cons of concave slides?

#18 Post by KurtM » Fri Feb 26, 2016 12:39 am

charlie g wrote:Hi, zzffnn...how did you 'over heat' the diatom slides? I don't understand. charlie guevara
Bet his lamps generated sufficient heat to soften the mountant and allow the cover slip to move around. OUCH. I have a whole bunch of Klaus Kemp slides, and some of them definitely have soft mountant (said the guy who isn't above crashing a lens into a cover slip occasionally -- and it's a bit shocking to see frustules moving once again, almost like Night Of The Living Dead - Revenge Of The Diatoms).

Once again I take this opportunity to urge everybody to store their slides horizontally, and not vertically as most slide boxes provide for (all ya gotta do is set the slide box on edge like a book).
Cheers,
Kurt Maurer
League City, Texas
email: ngc704(at)aol(dot)com
http://sawdustfactory.nfshost.com/microscopes/
https://www.flickr.com/photos/67904872@ ... 912223623/

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Re: Good Prepared Slides as examples of water life AND pros and cons of concave slides?

#19 Post by zzffnn » Fri Feb 26, 2016 1:03 am

Yes, I was testing some new DIY (non-Kohler) halogen lights and did not pay attention to shielding the heat. Then I either touched the softened mountant or did not store slides flat. Also, for my very first KK slide, objective crashed into it twice, once in my hands, once in hands of a local microscope dealer who was praising its beauty :shock:
Selling my Canon FD 200mm F/2.8 lens

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Re: Good Prepared Slides as examples of water life AND pros and cons of concave slides?

#20 Post by charlie g » Fri Feb 26, 2016 2:37 am

Wow...I run a 30 watt halogen in my work horse stand. You need more output for high magnification DF and CX POL?

If your illumination softened balsam at level of the stands stage...what sort of heat sink/cooling at the actual lamphouse?

And could your eyes feel the heat..or is it for your image capture devices and not direct visual observations? Softened balsam at level of the stage...yikes. charlie guevara

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Re: Good Prepared Slides as examples of water life AND pros and cons of concave slides?

#21 Post by Dale » Sat Feb 27, 2016 2:40 am

I plan to buy a Klaus Kemp test slide that you and others have mentioned Fan.
This ebay scope has an interesting photo of some diatoms, are they Kemp's?

http://www.ebay.com/itm/American-Optica ... Uh7KRjtskw

Dale
B&L Stereozoom 4. Nikon E600. AO Biostar 1820.

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Re: Good Prepared Slides as examples of water life AND pros and cons of concave slides?

#22 Post by KurtM » Sat Feb 27, 2016 3:59 am

charlie g wrote:balsam
Probably not balsam on a diatom slide. I'd say Pleurax or Zrax.
Cheers,
Kurt Maurer
League City, Texas
email: ngc704(at)aol(dot)com
http://sawdustfactory.nfshost.com/microscopes/
https://www.flickr.com/photos/67904872@ ... 912223623/

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