NPM for animal tissue

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MicroMan2
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Location: Canada,Alberta

NPM for animal tissue

#1 Post by MicroMan2 » Sun Apr 10, 2016 3:07 am

Anyone has tried to nail polish on stained animal tissues eg.(retina, muscles, etc.) with the mountant. I need to find affordable clearing and mounting resources with shipping to Canada. Any suggestions of tip are useful, thanks.

kinase
Posts: 66
Joined: Wed Jul 29, 2015 3:18 am

Re: NPM for animal tissue

#2 Post by kinase » Sun Apr 10, 2016 1:18 pm

You mean to seal the coverslip to the slide or as the actual mounting medium? I've used it to seal coverslips until I started using som hard setting mount.

MicroMan2
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Location: Canada,Alberta

Re: NPM for animal tissue

#3 Post by MicroMan2 » Sun Apr 10, 2016 2:27 pm

As the mountant it self

billbillt
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Re: NPM for animal tissue

#4 Post by billbillt » Sun Apr 10, 2016 3:34 pm

...
Last edited by billbillt on Sun Apr 10, 2016 5:32 pm, edited 1 time in total.

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Raul
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Location: Bradford, England

Re: NPM for animal tissue

#5 Post by Raul » Sun Apr 10, 2016 4:48 pm

I've been unsuccessful so far with Nail Polish, I tried to mount skeletal muscle stained with H&E but it cracked horribly after couple of days, or it made bubbles around, above and in the tissue when I tried to mount kidney samples. Best and most convenient is with Glycerol Jelly.

Peter
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Joined: Sun Oct 12, 2014 5:34 pm

Re: NPM for animal tissue

#6 Post by Peter » Sun Apr 10, 2016 8:02 pm

Hi MicroMan,
More canada balsam here http://www.surplusshed.com/pages/item/b1077.html than can be used in a lifetime. Some people look down on canada balsam these days but it's infinitely better than nail polish.
Hope this helps.
Peter.

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billben74
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Re: NPM for animal tissue

#7 Post by billben74 » Sun Apr 10, 2016 8:10 pm

Excuse me if I've got the wrong end of the stick here but perhaps you could use brunseal clear.

Here is a link to UK supplier (which I realise will not help you directly).

http://www.brunelmicroscopes.co.uk/stains.html

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billben74
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Re: NPM for animal tissue

#8 Post by billben74 » Sun Apr 10, 2016 8:20 pm

Apolgies now I've read the post I realise you meant as mountant :oops:

Can you get Numount? This is a canada balsam substitute.

DaveH
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Location: Bexhill on Sea

Re: NPM for animal tissue

#9 Post by DaveH » Mon Apr 11, 2016 9:47 am

MicroMan2 wrote:Anyone has tried to nail polish on stained animal tissues eg.(retina, muscles, etc.) with the mountant. I need to find affordable clearing and mounting resources with shipping to Canada. Any suggestions of tip are useful, thanks.
Hi I have had no luck with nail polish, I've tried it a few times and all the slides I made ended up in the bin.

For simple mounts by far my favourite is uv adhesive mount from alcohol, I have blood smears, pollen, onion skin all mounted in 2006 and holding up in perfect condition.

If you are looking for something simple and easy to obtain then maybe try gum arabic, found in art stores used in watercolour painting mount from water, needs ringing.

Damar varnish sold in art stores used in oil painting, often sold as double mastic varnish mount from white spirit or turpentine needs ringing for long term archiving.

Hop this is of some help.

Dave

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Raul
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Location: Bradford, England

Re: NPM for animal tissue

#10 Post by Raul » Tue Apr 12, 2016 9:45 pm

MicroMan2 I solved it, I mounted cartilage tissue in Nail Polish and it did not crack, nor it formed bubbles.
Here's my protocol:
After the staining, dehydrate in 70% IPA 1min, 90% IPA 1 min, 100% IPA 1 min then add the Nail Polish (I used one that had IPA as a solvent in it so it mixed perfectly)
Here are some nice chondrocytes, stained with TBO, mounted yesterday in Nail Polish as described above.
Image

Image

billbillt
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Re: NPM for animal tissue

#11 Post by billbillt » Tue Apr 12, 2016 11:35 pm

Yes, nail polish works well for me..
BillT

billbillt
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Re: NPM for animal tissue

#12 Post by billbillt » Mon Apr 15, 2019 3:37 pm

Those turned out very well!...

BillT


Last bumped by MicroMan2 on Mon Apr 15, 2019 3:37 pm.

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