NPM for animal tissue
NPM for animal tissue
Anyone has tried to nail polish on stained animal tissues eg.(retina, muscles, etc.) with the mountant. I need to find affordable clearing and mounting resources with shipping to Canada. Any suggestions of tip are useful, thanks.
Re: NPM for animal tissue
You mean to seal the coverslip to the slide or as the actual mounting medium? I've used it to seal coverslips until I started using som hard setting mount.
Re: NPM for animal tissue
As the mountant it self
Re: NPM for animal tissue
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Last edited by billbillt on Sun Apr 10, 2016 5:32 pm, edited 1 time in total.
Re: NPM for animal tissue
I've been unsuccessful so far with Nail Polish, I tried to mount skeletal muscle stained with H&E but it cracked horribly after couple of days, or it made bubbles around, above and in the tissue when I tried to mount kidney samples. Best and most convenient is with Glycerol Jelly.
Re: NPM for animal tissue
Hi MicroMan,
More canada balsam here http://www.surplusshed.com/pages/item/b1077.html than can be used in a lifetime. Some people look down on canada balsam these days but it's infinitely better than nail polish.
Hope this helps.
Peter.
More canada balsam here http://www.surplusshed.com/pages/item/b1077.html than can be used in a lifetime. Some people look down on canada balsam these days but it's infinitely better than nail polish.
Hope this helps.
Peter.
Re: NPM for animal tissue
Excuse me if I've got the wrong end of the stick here but perhaps you could use brunseal clear.
Here is a link to UK supplier (which I realise will not help you directly).
http://www.brunelmicroscopes.co.uk/stains.html
Here is a link to UK supplier (which I realise will not help you directly).
http://www.brunelmicroscopes.co.uk/stains.html
Re: NPM for animal tissue
Apolgies now I've read the post I realise you meant as mountant
Can you get Numount? This is a canada balsam substitute.
Can you get Numount? This is a canada balsam substitute.
Re: NPM for animal tissue
Hi I have had no luck with nail polish, I've tried it a few times and all the slides I made ended up in the bin.MicroMan2 wrote:Anyone has tried to nail polish on stained animal tissues eg.(retina, muscles, etc.) with the mountant. I need to find affordable clearing and mounting resources with shipping to Canada. Any suggestions of tip are useful, thanks.
For simple mounts by far my favourite is uv adhesive mount from alcohol, I have blood smears, pollen, onion skin all mounted in 2006 and holding up in perfect condition.
If you are looking for something simple and easy to obtain then maybe try gum arabic, found in art stores used in watercolour painting mount from water, needs ringing.
Damar varnish sold in art stores used in oil painting, often sold as double mastic varnish mount from white spirit or turpentine needs ringing for long term archiving.
Hop this is of some help.
Dave
Re: NPM for animal tissue
MicroMan2 I solved it, I mounted cartilage tissue in Nail Polish and it did not crack, nor it formed bubbles.
Here's my protocol:
After the staining, dehydrate in 70% IPA 1min, 90% IPA 1 min, 100% IPA 1 min then add the Nail Polish (I used one that had IPA as a solvent in it so it mixed perfectly)
Here are some nice chondrocytes, stained with TBO, mounted yesterday in Nail Polish as described above.
Here's my protocol:
After the staining, dehydrate in 70% IPA 1min, 90% IPA 1 min, 100% IPA 1 min then add the Nail Polish (I used one that had IPA as a solvent in it so it mixed perfectly)
Here are some nice chondrocytes, stained with TBO, mounted yesterday in Nail Polish as described above.
Re: NPM for animal tissue
Yes, nail polish works well for me..
BillT
BillT
Re: NPM for animal tissue
Those turned out very well!...
BillT
BillT
Last bumped by MicroMan2 on Mon Apr 15, 2019 3:37 pm.