Botanical

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exmarine
Posts: 247
Joined: Mon May 09, 2016 1:16 am
Location: Cambridgeshire UK

Botanical

#1 Post by exmarine » Wed Nov 30, 2016 4:16 pm

Hi members,
A member of a small group of microscopists has aske me to show them how to make a stained botanical slide. While I feel very honoured I know need to put my thinking cap on regarding wax, stains (strength, for how long to stain etc). I thought I would give a peep at what I have done a couple of years ago.
Attachments
Yew  TS-0001.JPG
Yew TS-0001.JPG (177.46 KiB) Viewed 2770 times
Lily-0007.JPG
Lily-0007.JPG (181.74 KiB) Viewed 2770 times
Field poppy 1.JPG
Field poppy 1.JPG (184.31 KiB) Viewed 2770 times
Cosmos  x.s.  bud.JPG
Cosmos x.s. bud.JPG (161.5 KiB) Viewed 2770 times
Thank you :shock:
Best regards
exmarine :x

uses Watson 'Service' 1950 compound.
uses Watson Stereo 1960 ish.

JimT
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Joined: Fri Oct 24, 2014 1:57 pm

Re: Botanical

#2 Post by JimT » Wed Nov 30, 2016 4:51 pm

Those are very nice.

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rnabholz
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Location: Iowa USA
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Re: Botanical

#3 Post by rnabholz » Wed Nov 30, 2016 9:26 pm

Very nice series exmarine.

The field poppy is really interesting.

What stain did you use on these?

Happy to see anymore you come across.

Rod

billbillt
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Joined: Mon Nov 10, 2014 10:01 pm

Re: Botanical

#4 Post by billbillt » Wed Nov 30, 2016 11:14 pm

Yes, these are very good!...

BillT

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billben74
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Location: Cambridge, UK

Re: Botanical

#5 Post by billben74 » Wed Nov 30, 2016 11:34 pm

Nice set. Really like the lily and the cosmos.
All really good though.

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exmarine
Posts: 247
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Location: Cambridgeshire UK

Re: Botanical

#6 Post by exmarine » Thu Dec 01, 2016 1:01 am

Hi Rod,
If my pitiful brain serves me well the brown would have been Bismark Brown and the red would have been Carmine that is in the Field Poppy and the Cosmos. What I like about B. Brown is that one can not overstain with it.
I now need to look up some of the protocols I used a while back as I will be honest with you all I have forgotten timings for the waxing and staining.

I have a couple more that I have bought and unfortuneatly I can not read the writing on the Victorian slides.
Attachments
Victorian slid can not read label-0003.JPG
Victorian slid can not read label-0003.JPG (141.2 KiB) Viewed 2731 times
Gp 2 slide made 1857 a.JPG
Gp 2 slide made 1857 a.JPG (176.55 KiB) Viewed 2731 times
Gp 2 lime tree 1.JPG
Gp 2 lime tree 1.JPG (158.46 KiB) Viewed 2731 times
Thank you :shock:
Best regards
exmarine :x

uses Watson 'Service' 1950 compound.
uses Watson Stereo 1960 ish.

Yvan
Posts: 92
Joined: Mon Oct 24, 2016 7:46 am

Re: Botanical

#7 Post by Yvan » Thu Dec 01, 2016 6:14 pm

.
Last edited by Yvan on Sun Dec 04, 2016 4:43 pm, edited 2 times in total.

charlie g
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Joined: Mon Oct 20, 2014 7:54 pm

Re: Botanical

#8 Post by charlie g » Fri Dec 02, 2016 1:04 am

Hi,exmarine, thanks for the botanical preps you offer here. I'm curious as to how long ago you made these botanical slides of yours? I'm curious if you can guess an age for the slides you purchased?

It's great your doing botanical microtechnique again...congrats on being an active member of a microscopy group in your area...and your doing botanical microtechnique for the group/ with the group.bravo.

Please visit this forum member: JohnB./Mr.sonchas tutorial posts in this forum. JohnB. has actual schedules for dehydration times, wax infiltration times..right on to Shandon microtome processing...staining schedules...and outcomes. Then JohnB./Mr.Sonchas gives his acellerated (less time, less steps) schedules of his botanical microtechnique. Often JohnB. mentions texts from which he applies techniques...and then he shares how/where he trims ( a botanical pun?!) the schedules.

I have terrific pleasure in following these botanical microtechnique paths to microscopy....now that I aquired a group of John B.s prepared slides...I always have the garden microscopy for when the world wearies and society ceases to satisfy ( oops, sorry Louisa May Acot...err..happy birthday to you).

please vist the threads in this forum for John B.'s schedules ('shedules' it sounds like in Canada?). charlie guevara

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