Moss Capsule sectioning for slide making

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janvangastel
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Re: Moss Capsule sectioning for slide making

#31 Post by janvangastel » Thu Jan 24, 2019 5:51 pm

Very interesting. I still have a long way to go before I completely understand this. Making such thin sections looks like a big challenge to me.

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mrsonchus
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Re: Moss Capsule sectioning for slide making

#32 Post by mrsonchus » Thu Jan 24, 2019 6:03 pm

Hobbyst46 wrote: May the power ("Schwartz" :lol: ) be with you.

I gonna need it! :D :D :D

John B.
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mrsonchus
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Re: Moss Capsule sectioning for slide making

#33 Post by mrsonchus » Thu Jan 24, 2019 6:08 pm

Hi Jan, hmm, it's a lot to learn but truly fascinating. Start with hand-sectioning - you'll be surprised how beautiful a hand-section can be with the application of a little Safranin stain.
Thanks for looking-in Jan.

p.s. check out the late great Walter Dioni's posts here in the forum archives - they are what started me off over three years ago now!

John B.
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Radazz
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Re: Moss Capsule sectioning for slide making

#34 Post by Radazz » Fri Jan 25, 2019 4:44 pm

Thank you so much, JohnB!
You are dispelling all of the mystery of this process.
Can’t wait for the next installment.
Radazz
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mrsonchus
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Re: Moss Capsule sectioning for slide making

#35 Post by mrsonchus » Fri Jan 25, 2019 6:44 pm

Radazz wrote:Thank you so much, JohnB!
You are dispelling all of the mystery of this process.
Can’t wait for the next installment.
Radazz
Ahhh Radazz... don't worry my friend, there are plenty of mysteries still to grapple with! :D :D

Thanks for your compliment my friend. I hope to make a fresh set of sections over the weekend now that I've established that the wax-blocks are viable. I also have a few Sunflower and Dandelion-root roughing-slides from this batch, all stained and mounted, just drying and stabilising - some images from them soon also.

John B. :D
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shawn perez
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Re: Moss Capsule sectioning for slide making

#36 Post by shawn perez » Fri Aug 14, 2020 6:48 pm

Hii, How do you store it once dried and cut into blocks?

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mrsonchus
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Re: Moss Capsule sectioning for slide making

#37 Post by mrsonchus » Fri Aug 14, 2020 7:23 pm

Hi shawn. If you mean the wax-blocks (containing specimen for sectioning), I use those cheap plastic divided boxes that sets of screws etc come in. As far as ambient environment, room-temp is fine.
The blocks really do keep well for several years at least, in my experience. I often have 'left over' tissue pieces when I've made a set of blocks from infiltrated tissue. I simply put these into a larger & deeper wax-block mold and let it set in the usual way - these I think of as my 'bulk blocks'. They are easily re-melted and the tissue embedded as if it's straight out of infiltration!

Here's an image of one of my (Dandelion root I think) 'bulk-blocks'...
Extra tissue stored in one big wax-block
Extra tissue stored in one big wax-block
root bulk block.jpeg (62.59 KiB) Viewed 621 times
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shawn perez
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Re: Moss Capsule sectioning for slide making

#38 Post by shawn perez » Fri Aug 14, 2020 8:59 pm

mrsonchus wrote:
Fri Aug 14, 2020 7:23 pm
Hi shawn. If you mean the wax-blocks (containing specimen for sectioning), I use those cheap plastic divided boxes that sets of screws etc come in. As far as ambient environment, room-temp is fine.
The blocks really do keep well for several years at least, in my experience. I often have 'left over' tissue pieces when I've made a set of blocks from infiltrated tissue. I simply put these into a larger & deeper wax-block mold and let it set in the usual way - these I think of as my 'bulk blocks'. They are easily re-melted and the tissue embedded as if it's straight out of infiltration!

Here's an image of one of my (Dandelion root I think) 'bulk-blocks'...
root bulk block.jpeg
This is really amazing... Thank you😄

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