MicrobeHunter.com Microscopy Forum

Hi everyone
I made some glycerin jelly today for making permanent slides. What is the procedure for the specimen if it is to be mounted in glycerin jelly? And how do i really mount it in the jelly? Do i heat it until it is liquid and pour some ok the slide? Very interested in glycerin jelly mounting as i hope to avoid Xylol a bit.

I have had some glycerin jelly I made sitting in my lab frig for years now. Very little discoloration. Although I may see some slight signs of microbial growth. LOL

Never got around to using it. I was trying a different preservative which has worked incredibly.

Look at page 30 of this document: https://www.berliner-mikroskopische-ges ... skopie.pdf

As far as I know you have to do something against fungus development when using glycerin gelantine for long term slides.

Bob

The method is to include a very small amount of 'disinfectant' in the formula, I used 'Listerine' with very good results indeed when I was experimenting with glycerin jelly mounts.
Here's a link to a post re glycerin jelly..

mrsonchus wrote:The method is to include a very small amount of 'disinfectant' in the formula, I used 'Lysterine' with very good results indeed when I was experimenting with glycerin jelly mounts.
Here's a link to a post re glycerin jelly..

It is your post that inspired me to try it. The thing is i dont know how to process the specimen (which chemical to use that is compatible with the jelly). And yes i did use Lysterine as you have. Very informative post you made. I will read Dionis post on it thank you!

einman wrote:I have had some glycerin jelly I made sitting in my lab frig for years now. Very little discoloration. Although I may see some slight signs of microbial growth. LOL

Never got around to using it. I was trying a different preservative which has worked incredibly.

Which one did you use, if i may ask?

MicroBob wrote:Look at page 30 of this document: https://www.berliner-mikroskopische-ges ... skopie.pdf

As far as I know you have to do something against fungus development when using glycerin gelantine for long term slides.

Bob

Thank you Bob very helpful as ever!

See here for method of use:-


http://www.microscopy-uk.org.uk/mag/ind ... part4.html

Yes I used Cepacol and the glycerin jelly i made has been almost 5 years in the frig and is only now showing very slight signs of potential microbial growth. I say potential because I have yet to examine what may only be some surface drying perhaps..pure conjecture. Bottom line the Cepacol works great.