Home made (Very Basic) Microtome
Re: Home made (Very Basic) Microtome
Hi Michael,
this is the type mostly used here:[imghttp://www.microbehunter.com/microscopy-forum/ ... d=13922img].
You can clamp the stem embedded in carrot pieces. My one is old, made by Sartorius.
Bob
this is the type mostly used here:[imghttp://www.microbehunter.com/microscopy-forum/ ... d=13922img].
You can clamp the stem embedded in carrot pieces. My one is old, made by Sartorius.
Bob
Re: Home made (Very Basic) Microtome
Thanks, Bob
I’ve not seen that style before.
... Every day is a learning day !!
MichaelG.
I’ve not seen that style before.
... Every day is a learning day !!
MichaelG.
Too many 'projects'
Re: Home made (Very Basic) Microtome
Hi Michael,
we here in Germany on the other hand side nomally never experience a Camebridge rocking microtome or copy of it. Regional differences.
For the cylinder hand microtome a blade holder is known for along time under the name SHK-Halter. It takes Leica 818 single use microtome blades. These have become more and more expensive and now cost 100€ per pack of 50 - unattractive in my eyes. To be able to do plant sections on a group meeting I developed my cutter blade holder.
It carries the edge in a distance to the table so the edge touches nothing but plant material.
Interestingly nobody was interested in my offer here. Probably there are fewer people doing plant sections with a cylinder microtome here.
Bob
we here in Germany on the other hand side nomally never experience a Camebridge rocking microtome or copy of it. Regional differences.
For the cylinder hand microtome a blade holder is known for along time under the name SHK-Halter. It takes Leica 818 single use microtome blades. These have become more and more expensive and now cost 100€ per pack of 50 - unattractive in my eyes. To be able to do plant sections on a group meeting I developed my cutter blade holder.
It carries the edge in a distance to the table so the edge touches nothing but plant material.
Interestingly nobody was interested in my offer here. Probably there are fewer people doing plant sections with a cylinder microtome here.
Bob
Re: Home made (Very Basic) Microtome
HI Bob.
Posts about plant sections seem to get very high numbers of 'views', but I don't think there are many people actually making plant sections. There are not many posts on plants, most seem to be about pond life.
Alan
Posts about plant sections seem to get very high numbers of 'views', but I don't think there are many people actually making plant sections. There are not many posts on plants, most seem to be about pond life.
Alan
Re: Home made (Very Basic) Microtome
Plant-section posts! Have a look over my posts -- over 3k posts re plants!
Hand-sectioned plants, May 2017, see my post.
Some great results are possible with hand-sectioning, even without a hand-microtome. I take mine without using a microtome, just the 'back of a fingernail' does the job.
Here's a more recent post, bamboo hand-sectioned...
Keep at it, the results are fascinating and well worth the small effort.
Hand-sectioned plants, May 2017, see my post.
Some great results are possible with hand-sectioning, even without a hand-microtome. I take mine without using a microtome, just the 'back of a fingernail' does the job.
Here's a more recent post, bamboo hand-sectioned...
Keep at it, the results are fascinating and well worth the small effort.
John B
Re: Home made (Very Basic) Microtome
Hi John,
nobody will have forgotten your botanical sections, I'm sure! I always enjoy your threads a lot and read them carefully.
But apart from you there are few members here who post on botanical sectioning and staining.
In the german forum there are a lot of people who make plant sections and a few who are very productive. Most use AFE fixated material, carrot enclosing, cylinder microtome, blade holder, Etzold or Wacker differentiating stain. They have developed a certain method with little variation and concentrate on the subjects. A couple of members write really nice reports that are very informative. After covering many frequent and well known plants they show more and more very special plants:https://www.mikroskopie-forum.de/index. ... ic=27181.0
Early this year we had group meeting about plant sectioning and staining and had an absolute record attendance. The lab looked as if a wheelbarrow full of carrots had been bombed!
Maybe there is just a lot of interest in plant sectioning in Germany?
Bob
nobody will have forgotten your botanical sections, I'm sure! I always enjoy your threads a lot and read them carefully.
But apart from you there are few members here who post on botanical sectioning and staining.
In the german forum there are a lot of people who make plant sections and a few who are very productive. Most use AFE fixated material, carrot enclosing, cylinder microtome, blade holder, Etzold or Wacker differentiating stain. They have developed a certain method with little variation and concentrate on the subjects. A couple of members write really nice reports that are very informative. After covering many frequent and well known plants they show more and more very special plants:https://www.mikroskopie-forum.de/index. ... ic=27181.0
Early this year we had group meeting about plant sectioning and staining and had an absolute record attendance. The lab looked as if a wheelbarrow full of carrots had been bombed!
Maybe there is just a lot of interest in plant sectioning in Germany?
Bob
Re: Home made (Very Basic) Microtome
Great link Bob - some great examples there! There's nothing more colourful than a hand-section! Some truly beautiful examples, and I love the labelling and interest.
I may have to have a look at that forum. What's the German for 'Hello'!
Many thanks Bob. A superb lead.
I may have to have a look at that forum. What's the German for 'Hello'!
Many thanks Bob. A superb lead.
John B
Re: Home made (Very Basic) Microtome
Hi Bob.
There is indeed many plant studies on this site.
See here for a linked list of all the posts on plant microscopy. A virtual Alladin's cave. From A to Z
https://www.mikroskopie-forum.de/index. ... ic=21220.0
There is indeed many plant studies on this site.
See here for a linked list of all the posts on plant microscopy. A virtual Alladin's cave. From A to Z
https://www.mikroskopie-forum.de/index. ... ic=21220.0
Re: Home made (Very Basic) Microtome
Hi John 'Guten Tag'
Edit:- You could always use google translate to see what they are talking about. Just copy and paste the text into here:-
https://translate.google.com/
Edit:- You could always use google translate to see what they are talking about. Just copy and paste the text into here:-
https://translate.google.com/
Re: Home made (Very Basic) Microtome
Sounds-good!
I'll have a visit later, maybe even join-up.
I have to take the dog to the vet' soon..... bad teeth and partial deafness, the dog that is, not me!
Thanks agin Bob, I'll let you know how I get on.
I'll have a visit later, maybe even join-up.
I have to take the dog to the vet' soon..... bad teeth and partial deafness, the dog that is, not me!
Thanks agin Bob, I'll let you know how I get on.
John B
Re: Home made (Very Basic) Microtome
Only problem: Whatever you want to section, somebody has done it before!Roldorf wrote: ↑Tue Oct 08, 2019 12:25 pmHi Bob.
There is indeed many plant studies ...From A to Z
https://www.mikroskopie-forum.de/index. ... ic=21220.0
Re: Home made (Very Basic) Microtome
So can we safely assume that the vet is healthy ?
MchaelG.
Too many 'projects'