MicrobeHunter.com Microscopy Forum

Nikon Microphot stepper adapter

STL file:
https://www.thingiverse.com/thing:4149920

Nicely done, Saul. Looks like you're revolutionizing things, one STL file at a time.

PeteM wrote: ↑

Fri Feb 07, 2020 10:27 pm

Nicely done, Saul. Looks like you're revolutionizing things, one STL file at a time.

Thanks, Pete !

Hi Saul,

What workflow are you using with such a set up? As in, do you zero at the lowest level and then motor away while video-ing, then use toupview to z-focus the resulting movie file?

I'm curious what people out there use for software/controllers and how they prevent crashing the objective.

Thanks,
Ted

microb wrote: ↑

Mon Feb 10, 2020 9:17 pm

...What workflow are you using with such a set up? As in, do you zero at the lowest level and then motor away while video-ing, then use toupview to z-focus the resulting movie file?
I'm curious what people out there use for software/controllers and how they prevent crashing the objective...

Hi Ted !
I'm using WeMacro controller. Yes, focus at the lowest point (little bit more, in order to catch lowest focus point), setting as a start point in the controller, next - to the highest point (setting end point in the controller), step size is already preset, taking bunch of photos (not a video), next - stacking in Zerene or Photoshop. Same story with my DIY vertical or horizontal setups.