Unidentified structures in lichen. A diatom?

[nastur74]

Hello
I rencently observed a lichen sample from an oak tree. I could see the hyphae and the single-celled algae. Among the debries I found some structures I could not identify. Anyone can help me? Are they diatoms? They are the brown structures.
Thank you


Magnification: 720 x

https://1drv.ms/i/s!An2CX5lcY0mZl1PWvhX ... H?e=LBAFmO
https://1drv.ms/i/s!An2CX5lcY0mZl1L4lTI ... A?e=ZGIedB

[Bruce Taylor]

The first one is the spore of an ascomycete fungus. The second one might be fungal as well, though I don't recognize it (not a diatom, in any case).

[nastur74]

Thank you very much!! I did not know these shapes and color of spores.
Greatings

[apochronaut]

The second one is a size 5 , women's , Lilliputian clog shoeprint.
















Just kidding. That's a wild yeast..... maybe schizosaccharomyces or commonly called a fission yeast. Fission yeasts longitudinally divide like bacteria. The line separating the heel from the sole of the shoeprint is the division between the parent and daughter cell.

[charlie g]

Hi, nastur 74...thanks for this post. I ask you: 1) how did you collect the lichen sample from oak tree? Did you shave off a segment of the lichen down to the bark of the tree it flourished on?

It sounds odd..but when collect a specimen of lichen...it is similar to a biopsy collection of skin lesion a doctor employs.

What is your approach to lichen specimen collection...do you collect an entire lichen segment ( like a doctor might biopsy a lesion on a persons skin)?

[nastur74]

Hello and thank you all for answering.
My 7-year-old daughter pinched the sample with her own fingers to later make a video for our microscopy channel. She loves to look through the eyepieces and have her family and friends listen to her on YouTube. I know that in the sample I have to look for hyphae and unicellular algae,...but I was surprised to see those structures.