From the usual pond in my backyard...

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tonikon
Posts: 87
Joined: Sun Nov 29, 2020 10:11 am

From the usual pond in my backyard...

#1 Post by tonikon » Sun Dec 20, 2020 9:17 am

Hello everyone and happy Sunday ...
in the usual pond in my backyard, today I photographed these two organisms.
Could anyone give them a name?
The photos are not very good because I have no phase contrast but only brightfield.
Trying to work for oblique lighting.
Ciao
Toni

P.S. sharpness isn't great...today no APO, only NPL/PL objectives... :(
Attachments
ND Leitz SM NPL16x 001.jpg
ND Leitz SM NPL16x 001.jpg (107.27 KiB) Viewed 3055 times
ND Leitz SM PL40x 002.jpg
ND Leitz SM PL40x 002.jpg (192.05 KiB) Viewed 3055 times

viktor j nilsson
Posts: 761
Joined: Thu Aug 23, 2018 10:12 pm
Location: Lund, Sweden

Re: From the usual pond in my backyard...

#2 Post by viktor j nilsson » Sun Dec 20, 2020 12:58 pm

I think these are a lot nicer than the vast majority of phase contrast photos I've seen. I just don't find PC visually appealing with the halos. Great for observation, though. But I don't think you need to worry about that.

I did notice right away that these weren't taken with your Apo objectives. How come?

Have you started experimenting with Pol, Oblique, DF and GUF? I think you'll do wonderful things with these techniques, given your clear visual talent.

tonikon
Posts: 87
Joined: Sun Nov 29, 2020 10:11 am

Re: From the usual pond in my backyard...

#3 Post by tonikon » Sun Dec 20, 2020 6:35 pm

Thank you very much for your appreciation... I really need a big encouragement because these are my first microscope images and everything seems difficult to me. Indeed, brightfield seems like a rewarding "visual" experience to me too, but I'd like starting to play with the oblique lighting. Where can I find a good guide explaining the differences, advantages-disadvantages and DIY tricks for Pol, oblique, DF and GUF? ... I admit that I don't even know the meaning of some acronyms...

P.S. Today, no APO only because I'm playing/testing with my old NPL/PL objectives...you can see them in post
viewtopic.php?f=25&t=11176
Ciao
Toni

tonikon
Posts: 87
Joined: Sun Nov 29, 2020 10:11 am

Re: From the usual pond in my backyard...

#4 Post by tonikon » Sun Dec 20, 2020 6:49 pm

Just photographed two other micro-organisms from the same pond. One just seems to be a tardigrade ... I got really excited, because I love them and had never seen one before. The second organism, rather large, had a very showy green color and compared to the organisms it looked like a giant!
Attachments
Tardigrada 2020.12.20 Leitz APO 12.5.jpg
Tardigrada 2020.12.20 Leitz APO 12.5.jpg (157.4 KiB) Viewed 3011 times
ND 2020.12.20 Leitz APO 12.5.jpg
ND 2020.12.20 Leitz APO 12.5.jpg (168.44 KiB) Viewed 3011 times

BramHuntingNematodes
Posts: 1546
Joined: Tue Jan 21, 2020 1:29 am
Location: Georgia, USA

Re: From the usual pond in my backyard...

#5 Post by BramHuntingNematodes » Sun Dec 20, 2020 7:09 pm

I should look for some larger freshwater stuff sometime I been looking at chlamydomonas lately and its adorable but p. small
1942 Bausch and Lomb Series T Dynoptic, Custom Illumination

tonikon
Posts: 87
Joined: Sun Nov 29, 2020 10:11 am

Re: From the usual pond in my backyard...

#6 Post by tonikon » Mon Dec 21, 2020 5:50 am

Could the second specimen be a Stentor? It was really very big ...

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micro
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Re: From the usual pond in my backyard...

#7 Post by micro » Mon Dec 21, 2020 6:05 pm

It does look like a stentor

Bruce Taylor
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Joined: Fri Sep 04, 2015 11:34 am

Re: From the usual pond in my backyard...

#8 Post by Bruce Taylor » Mon Dec 21, 2020 6:51 pm

Nice pics! First one is a slightly squashed Spirostomum (it has a moniliform macronucleus & short peristome, so S. minus is likely). Second image is a slightly squashed Paramecium. Compression has changed the cell shape a bit, so it resemble Frontonia (a closely related genus); however, the tuft of cilia at the posterior and the appearance of the faintly-visible mouth opening are more typical of Paramecium. The blue-green Stentor is Stentor coeruleus.

PeteM
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Location: N. California

Re: From the usual pond in my backyard...

#9 Post by PeteM » Mon Dec 21, 2020 10:23 pm

Just a note of thanks, Bruce, for your many expert contributions. Thank you!

tonikon
Posts: 87
Joined: Sun Nov 29, 2020 10:11 am

Re: From the usual pond in my backyard...

#10 Post by tonikon » Tue Dec 22, 2020 4:54 am

Bruce...your competence is second only to your generosity! Thank you very much...

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