Holes in red blood cell?

[Octane80]

Ok, while having a look at some blood the other day It appeared as though some of my RBC had holes in them. I shifted the lighting a bit and it became even more apparent. I believe I was using 65x with some digital zoom with darkfield/oblique. Is this something any of you have ever come across?

[Octane80]

Also, here is the video taken previous to the photo. Seems as though there are some strange things going on. https://www.youtube.com/watch?v=C6v4Mst4S7g

[SunshineLW]

The shape of the mammalian red blood cell (RBC) is classically a "Biconcave Disc." Depending on a number of factors, these discs have different degrees of biconcavity, which creates what is known as "central pallor." The central pallor is caused by the relative thinness of the area created by the cell's biconcave shape. The degree of central pallor is affected by many factors, including (but not limited to): species, amount of hemoglobin in the RBC, where in the blood smear the RBCs are observed, and pathology.

For example, canine RBCs have much more central pallor compared to feline RBCs.

- Canine RBC:

- Feline RBC: