Tiny and Tinier?

Have problems identifying an organism? Ask for help here.
Post Reply
Message
Author
FLReef32043
Posts: 20
Joined: Sun Nov 25, 2018 12:40 am

Tiny and Tinier?

#1 Post by FLReef32043 » Mon Dec 10, 2018 2:20 am

Need some help with these two. No way for me to get any closer as they move in too large a pattern or two fast. Any ideas?

https://youtu.be/IQu-jwxomfg

User avatar
75RR
Posts: 6998
Joined: Sun Oct 12, 2014 2:34 am
Location: Estepona, Spain

Re: Tiny and Tinier?

#2 Post by 75RR » Mon Dec 10, 2018 5:50 am

charlie g's slow'm down recipe: 50/50 (half specimen fluid + half 1.5% concentration methyl cellulose)

This is the stuff: https://www.homesciencetools.com/produc ... ose-30-ml/
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

mnmyco
Posts: 142
Joined: Tue Aug 28, 2018 11:03 pm

Re: Tiny and Tinier?

#3 Post by mnmyco » Mon Dec 10, 2018 2:37 pm

Don't think we can ID from that video. Buy some of the stuff suggested. If you want to buy methyl cellulose and make your own, here is how. I make a 2% solution


Materials
Beaker
stirring hotplate--This is actually very important
stir bar
distilled or spring water
methyl cellulose (I use 4000 cst, I think. Have to check the bottle)
scale

Methods
Place 100 mL of the water and the stir bar into a beaker
Bring the water to a boil while rapidly stirring, and I mean as rapid as you can
Turn off the heat
As soon as the water stops boiling begin slowly adding 2g of methyl cellulose
Keep stirring until the solution is room temp.

Why bring to a boil? Methyl cellulose is a bit odd. It's solubility increases as temps decrease. If you add it to room temp water then it will gel on the outside and form large clumps. These things are super annoying and super hard to get rid of. By adding it near 100 C and while rapidly stirring you keep it suspended and evenly distributed in the water. This way as it cools it will cool as a homogeneous mixture without clumps. Takes forever, but it is by far the best method I have read/used.


The above protocol should be applied to carboxymethylcellulose as well, if I recall correctly.

mnmyco

Post Reply