Culture methods for invertebrate animals; by Galtsoff, Paul Simon, 1937

[75RR]

An old yet interesting book on this subject.

https://archive.org/details/culturemethodsfo00galt

Extract:

"A CULTURE MEDIUM FOR HYDATINA SENTA*
Josephine C. Ferris, University of Nebraska

BRING to the boiling point in 1oo cc. of water 1 gram of urea crystals, 1 gram of dried blood, and 1 gram of dried ox gall. Filter and add 3cc.of this triple solution to 1oocc. of tap or rainwater. This solution is very satisfactory for pedigree cultures of this rotifer in watch glasses if fed on Polytoma cultures. [See p. 61.]
If the Polytoma is cultured in a bone meal and hay solution it should be washed at least twice by centrifuging and decanting.

References
For the culture of Hydatina asplanchna see p. 210. For the culture of Philodina see p. 143."

[Crater Eddie]

I have been looking for creative uses for dried blood and ox gall, thanks!
But seriously, it does look interesting, thanks for the link.
CE