MicrobeHunter.com Microscopy Forum

Various adaptations of a Canon EOS 450D ... 650D ... 750D to a microscope

http://www.mikroskopie-ph.de/index-canon.html

Thanks 75RR for posting this contribution by Peter Hubel.
This comparison provides useful data, but raises questions as well.

First, most of the results were obtained with planapo objectives, although I believe that many hobbyists rely on less expensive and sophisticated objectives.

The very fine performance Olympus NFK dedicated photo eyepieces with Olympus objectives is as expected.

For the Zeiss microscopes, it appears that the only high-quality results are obtained with either:
1) specific afocal setups (variantes 1, 2, 3),
2) direct projection with dedicated Zeiss adapters (variantes 5, 6).
AFAIK, the Zeiss S-KPL eyepiece and Zeiss mipro lenses are very expensive.

Whereas focal (projection) with normal eyepieces that are raised above the rim of the photo tube is less satistacftory (variantes 13, 14).

What I miss in those results, and would like to see, is a demonstration of the 8X KPL with a series of Zeiss objectives and a 50mm camera lens, not 40mm as in variant no 2.
And hopefully, more experienced photomicrographers than me comment about the results shown in the link. What is acceptable and what is not.

I got very good results with direct projection using a normal kpl eyepiece. I changed over to afocal mainly to avoid dust problems as I could not connect the camera physically to the microscope due to vibrations and the flexible coupling I came up with was not ideal.

Having said that I am now having problems with a hot spot with the Olympus OM lenses. I suppose I will have to try another make.


Mipro or Projectiv eyepieces as the newer ones are called can be found for fairly reasonable amounts of money on occasion, but as always on ebay it takes time to find a suitably priced item.

Sometimes quite a long time. I have a 63x Projectiv and have been keeping an eye out for an 80, but have not found one yet at a price I am willing to pay.

Hobbyst46 wrote: ↑

Sat Sep 07, 2019 4:49 pm

What I miss in those results, and would like to see, is a demonstration of the 8X KPL with a series of Zeiss objectives and a 50mm camera lens, not 40mm as in variant no 2.

Does not number 3 give you that, though admittedly with just the one objective.

However, if it works well with one objective it should work well with others of the same make even if not of the same quality.

Peter Hubel is using a 10x for testing and it makes sense that he would use the best one he has available.


Note: If you look carefully at image #3 you can see that it has a hot spot. I wonder if that is because the Olympus ZUIKO 50mm/1:1,4 used is in fact part of the OM system.

Thanks 75RR,

75RR wrote: ↑

Sat Sep 07, 2019 5:33 pm

Hobbyst46 wrote: ↑

Sat Sep 07, 2019 4:49 pm

What I miss in those results, and would like to see, is a demonstration of the 8X KPL with a series of Zeiss objectives and a 50mm camera lens, not 40mm as in variant no 2.

Does not number 3 give you that, though admittedly with just the one objective.

Yes, no 3 gives it with the planapo 10X/0.32. I thought about the same test with other objectives, plan or neofluar.

However, if it works well with one objective it should work well with others of the same make even if not of the same quality.

That answers my question.

Note: If you look carefully at image #3 you can see that it has a hot spot. I wonder if that is because the Olympus ZUIKO 50mm/1:1,4 used is in fact part of the OM system.

Yes, I see a hot spot, but in my my opinion it is weaker than the one in variante 4.