My Zeiss Microscopes

What equipment do you use? Post pictures and descriptions of your microscope(s) here!
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Wes
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Re: My Zeiss Microscopes

#61 Post by Wes » Tue May 28, 2019 6:41 pm

Exactly! Nice drawing too!

If you rotate the polarizer in this setup would you see light diminution indicative of polarization.

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ImperatorRex
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Re: My Zeiss Microscopes

#62 Post by ImperatorRex » Mon Sep 23, 2019 8:58 pm

I am very proud to present my second "non Zeiss" member in the microscope family: An Olympus BX60 microscope. This should be really my very very last scope:

Image

Packing was taken seriously ... 114lb/52kg with wooden crate - air freight from oversea, has some costs but it safely and well arrived.
Image

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75RR
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Re: My Zeiss Microscopes

#63 Post by 75RR » Mon Sep 23, 2019 9:08 pm

Congratulations! Very nice looking. Is that the DIC slider I see above the nosepiece?

As you say - well packed
This should be really my very very last scope
They do say never say never ...
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

Wes
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Re: My Zeiss Microscopes

#64 Post by Wes » Tue Sep 24, 2019 6:00 pm

Very cool! Is it new, sure looks like it. What lenses and filters does it come with?

Must be quite exciting at your place with such a beautiful array of different scopes :D

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Re: My Zeiss Microscopes

#65 Post by ImperatorRex » Tue Sep 24, 2019 6:38 pm

Hi 75RR,
thank you - yes it has DIK. Took quite long until I decided to buy this microscope - so hardly a day or night without dreaming about this microscope. Finally...dreams become true.

Dear Wes,
still an old scope, must be something like 20 or more years old I guess. But like new after fixing few smaller things & cleaning (re-greasing the noise piece and one eyepiece).
It has the older UIS objectives fitted UPLFL (10,20,40 + 100) and Fluorescense cubes for UV, Blue and Green erection are in the turret.

Main reason to get this nice scope was the possiblity to directly capture the full corrected intermediate image directly on the camera sensor (without okular/projective or camera objective).

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Re: My Zeiss Microscopes

#66 Post by ImperatorRex » Wed Sep 25, 2019 10:37 am

A quick comparision of the Photo-Adaptions:

a) Olypus BX60 - UPlanFL40/0.75 PH2 - Canon EOS 600 (with flash)
Focus on Pleurosigma angulatum:
Image

b) Zeiss IM35 - Plan Neofluar 40/0,90 Imm - SKpl W10/20 - YN35mm F2 - Canon EOS 650 (with flash)
Focus on Pleurosigma angulatum:
Image

c) Olypus BX60 - UPlanFL40/0.75 PH2 - Canon EOS 600 (with flash)
Focus on Stauroneis phoenicenteron:
Image

d) Zeiss IM35 - Plan Neofluar 40/0,90 Imm - SKpl W10/20 - YN35mm F2 - Canon EOS 650 (with flash)
Focus on Stauroneis phoenicenteron:
Image
Last edited by ImperatorRex on Wed Sep 25, 2019 10:47 am, edited 2 times in total.

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75RR
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Re: My Zeiss Microscopes

#67 Post by 75RR » Wed Sep 25, 2019 10:46 am

All being equal and calculated by thumb, is that a 50% difference in magnification between the photo adapters?

Be interesting if you increased the magnification of the UPlanFL40/0.75 PH2 by that amount to compare the objectives.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

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Re: My Zeiss Microscopes

#68 Post by ImperatorRex » Wed Sep 25, 2019 10:54 am

Hi 75RR,
think the important difference is the Field of View - the adaption with the Olympus allows to capture the full FOV 22+, while the Zeiss finite has less FOV and crops a rectangle out of the FOW of 20.
Direct image of the intermediate image to the sensor of the camera also mean that is is less sensible to dirt allocated in between.

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Re: My Zeiss Microscopes

#69 Post by ImperatorRex » Wed Sep 25, 2019 11:07 am

Some corpped images:

Olympus:
Image

Zeiss:
Image

Obviously the Zeiss has some advantage due to higher n.A. and the water immersion. Also cover glass of the testplate has only thickness of 0.13, so the correction collar of the Zeiss Objective makes some difference to abopt the S.A.

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Re: My Zeiss Microscopes

#70 Post by 75RR » Wed Sep 25, 2019 3:08 pm

ImperatorRex wrote:
Wed Sep 25, 2019 10:54 am
Hi 75RR,
think the important difference is the Field of View - the adaption with the Olympus allows to capture the full FOV 22+, while the Zeiss finite has less FOV and crops a rectangle out of the FOW of 20.
Direct image of the intermediate image to the sensor of the camera also mean that is is less sensible to dirt allocated in between.
You are quite right of course. That was what you were showing us.

Thanks for the comparison (which I have placed side by side and reduced a bit, hope that is alright.) I think the Olympus holds up very well!


Note: So that both images would be bias positive I rotated the Zeiss image 180 - that might account for some perceived differences.
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Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

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Re: My Zeiss Microscopes

#71 Post by ImperatorRex » Wed Sep 25, 2019 8:03 pm

Well done 75RR, much better to compare. Also think the Olympus is doing quite well, also considering it is a phase contrast objective.

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75RR
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Re: My Zeiss Microscopes

#72 Post by 75RR » Wed Sep 25, 2019 8:26 pm

ImperatorRex wrote:
Wed Sep 25, 2019 8:03 pm
Well done 75RR, much better to compare. Also think the Olympus is doing quite well, also considering it is a phase contrast objective.
Indeed. Phase and 0.75NA, doing very well!
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

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Re: My Zeiss Microscopes

#73 Post by Wes » Thu Sep 26, 2019 7:55 pm

Did you use oil or other immersion medium with the Neofluar objective?

You get very nice quality with both objective, it'd be interesting to see some images you take with your new microscope, it looks very promising ;)

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Re: My Zeiss Microscopes

#74 Post by ImperatorRex » Thu Sep 26, 2019 8:53 pm

Hi Wes,
the Zeiss Plan Neofluar 40/0,90 is a multi-immersion objective, so the collar allows to use the immersion medium of your choice: oil, water or glycerin. Water is very convenient to use, its very easy to clean. I usually prefer the the dry objective Zeiss Planapo 40/0,95 but it has a bit a gradient in the DIC background, so I was using the Plan Neofluar instead to give the Zeiss a good chance against the Olympus :-)

Yes, will do some first photos. The flash setup at the Olympus is a bit provisionally, but seems to be good enough to start with photos. I have not really good pond samples, so probabely will go for some Haematococcus pluvialis algae...they are quick to re-activate from dried aplanospores. Maybe some direct comparisons then between the DIC from Olympus against the old Zeiss. What I have seen is that the Olympus has a very nice & strong contour contrast, were the Zeiss is much softer. Lets see how this looks on the photos.

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My Zeiss Microscopes (Comparison photo adaption Zeiss Standard versus Olympus BX)

#75 Post by ImperatorRex » Sat Oct 19, 2019 6:47 pm

Hi,
did a comparison of my photo adaptions I use currently:

green rectangle: Olympus BX with Canon EOS600D (the intermediate image is directly projected to the camera sensor)
red rectangle: Zeiss Standard with large field optovar 0,8x - KPL SKPL 10x/20 (Ocular) - YN EF35mm (Camera objective) - Canon EOS650D
blue rectangle: Zeiss Standard - KPL SKPL 10x/20 (Ocular) - YN EF35mm (Camera objective) - Canon EOS650D

Image

The BX photo adaption does even capture a bit more width of the FOV than is visible in the eyepieces (FOV22).

Below the single images taken with the 10x objectives:

Olympus BX UPlanFL 10x/0,30 PH1
Image

Zeiss Standard PlanApo 10/0,32 (with Optovar 0,80)
Image

Zeiss Standard PlanApo 10/0,32 (with Optovar 1,0)
Image

Some other photos

Pleurosigma angulatum (in air):

Olympus BX UPlanFL 10x/0,30 PH1:
Image

Zeiss Standard PlanApo 10/0,32 (with Optovar 0,80)
Image

Olympus BX UPlanFL 40x/0,75 PH2
Image

Zeiss Standard PlanApo 40/0,95 (with Optovar 0,80)
Image

Zeiss Standard PlanApo 40/0,95 (with Optovar 1,0)
Image

Olympus BX UPlanFL 10x/0,30 PH1:
(Scyllium young Shark)
Image

Zeiss Standard PlanApo 10/0,32 (with Optovar 0,80)
Image

Zeiss Standard PlanApo 10/0,32 (with Optovar 1,0)
Image

Hobbyst46
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Re: My Zeiss Microscopes

#76 Post by Hobbyst46 » Sat Oct 19, 2019 7:34 pm

Interesting comparison. What I personally take from it:

1. The S-KPL is a very good eyepiece for photography (as previously shown by others) - no wonder it is rare and very expensive
2. In spite of the 20-30 years age difference and the infinity optics of the BX, and of course the huge price difference, the Zeiss Standard yields about the same excellent performance as the BX (for brightfield !).

And a question please. Why a 35mm camera lens and not a 40mm lens ? considering the 10X eyepiece and APS-C sensor ?
Zeiss Standard GFL+Canon EOS-M10, Olympus VMZ stereo

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Re: My Zeiss Microscopes

#77 Post by ImperatorRex » Sat Oct 19, 2019 7:53 pm

Hi Hobbyst46,
definitely the Zeiss Standard still performes very well, respectively if equipped with Planapos - and yes it will cost only a fraction of the invinite systems.

From my experience I do not think that the SKPL 10x/20 makes a much better performance compared to a KPL 10x/20. So it is a nice to have but not really a must.
A cheap Photoadaption with good results is also a KPL 8x/18 with a 50mm Camera objective. Of coarse a slightly smaller FOV.

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