Zeiss Standard 14

What equipment do you use? Post pictures and descriptions of your microscope(s) here!
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Hobbyst46
Posts: 2191
Joined: Mon Aug 21, 2017 9:02 pm

Re: Zeiss Standard 14

#61 Post by Hobbyst46 » Sat Mar 16, 2019 7:04 pm

Sharpness of focus is best determined froma single image of z thin specimen, rather than a stack.
Zeiss Standard GFL+Canon EOS-M10, Olympus VMZ stereo

MicroBob
Posts: 1550
Joined: Sun Dec 25, 2016 9:11 am

Re: Zeiss Standard 14

#62 Post by MicroBob » Sat Mar 16, 2019 7:08 pm

Hi Enzo,
some thoughts about you 40:1

- the image is generally less impressive with the 40:1 than the 10:1
- the 40:1 has very shallow depth of field, your object needs to be flat
- the 40:1 needs the object directly below a 0,17mm cover slip for best quality
- the 40:1 is more susceptible to become dirty or damaged as it is so close to the cover slip

You might try with a specifically made, very flat test object like a piece of thin paper in water under the cover slip.

Bob

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