My first successful use of a microtome

What equipment do you use? Post pictures and descriptions of your microscope(s) here!
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DonSchaeffer
Posts: 190
Joined: Sun Mar 22, 2020 10:06 am
Location: Winnipeg, Manitoba, Canada

My first successful use of a microtome

#1 Post by DonSchaeffer » Sun Mar 29, 2020 12:57 pm

I'm just a beginner at trhe age of 80.
This is my first reasonably successful use of the microtome
Angular cells of a pearl plant leaf.
cells2[1].jpg
cells2[1].jpg (64.6 KiB) Viewed 824 times
caroy cells[2].jpg
caroy cells[2].jpg (69.23 KiB) Viewed 824 times

MicroBob
Posts: 1987
Joined: Sun Dec 25, 2016 9:11 am
Location: Northern Germany

Re: My first successful use of a microtome

#2 Post by MicroBob » Sun Mar 29, 2020 2:07 pm

Hi Don,
can you show more about your sectioning, perhaps a photo of you microtome and specimen?
Your photos look good but I'm not quite sure what I see.

Bob

DonSchaeffer
Posts: 190
Joined: Sun Mar 22, 2020 10:06 am
Location: Winnipeg, Manitoba, Canada

Re: My first successful use of a microtome

#3 Post by DonSchaeffer » Mon Mar 30, 2020 11:13 pm

Will do. Coming up.

DonSchaeffer
Posts: 190
Joined: Sun Mar 22, 2020 10:06 am
Location: Winnipeg, Manitoba, Canada

Re: My first successful use of a microtome

#4 Post by DonSchaeffer » Tue Mar 31, 2020 12:48 am

I have a low cost hand-held microtome. I noticed that the elevator did not have sufficient range to bring my samples to the surface so I added a little foam rubber plug in the bottom of the elevator. I also use a similar plug to cut samples to fit in the elevator. The knife that came with the microtome is like an old-fashioned barber's razor. It appears to be sufficiently sharp.
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BramHuntingNematodes
Posts: 138
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Location: Georgia, USA

Re: My first successful use of a microtome

#5 Post by BramHuntingNematodes » Tue Mar 31, 2020 1:34 am

That's nice work, Don. Maybe someday I'll get my act together and we could trade slides.
1942 Bausch and Lomb Series T Dynoptic, Custom Illumination

DonSchaeffer
Posts: 190
Joined: Sun Mar 22, 2020 10:06 am
Location: Winnipeg, Manitoba, Canada

Re: My first successful use of a microtome

#6 Post by DonSchaeffer » Wed Apr 01, 2020 1:15 am

I'd love that!

MicroBob
Posts: 1987
Joined: Sun Dec 25, 2016 9:11 am
Location: Northern Germany

Re: My first successful use of a microtome

#7 Post by MicroBob » Thu Apr 02, 2020 1:17 pm

Hi Don,
a useful material to embed plant parts in is a simple carrot. If you are interested in this method I can explain how to prepare the carrot.
Here in Germany we use blade holders for the use with cylinder microtomes. The advantage is that the edge of the blade never touches the surface of the table and stays sharp as long as possible. Your knife actually looks like a proper razor knife, not the junk that is often sold with cylinder microtomes today.

Bob

DonSchaeffer
Posts: 190
Joined: Sun Mar 22, 2020 10:06 am
Location: Winnipeg, Manitoba, Canada

Re: My first successful use of a microtome

#8 Post by DonSchaeffer » Thu Apr 02, 2020 8:22 pm

Where do you get one of those blades?

MicroBob
Posts: 1987
Joined: Sun Dec 25, 2016 9:11 am
Location: Northern Germany

Re: My first successful use of a microtome

#9 Post by MicroBob » Fri Apr 03, 2020 5:58 am

Hi Don,
I actually sell these holders from time to time: viewtopic.php?f=16&t=7065&p=62601&hilit ... der#p62601
Very good blades are the ones from OLFA,Japan, the inventor of this kind of knifes with break-off blades.

Bob

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