Levelling My Microscope

What equipment do you use? Post pictures and descriptions of your microscope(s) here!
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DonSchaeffer
Posts: 709
Joined: Sun Mar 22, 2020 10:06 am
Location: Winnipeg, Manitoba, Canada

Levelling My Microscope

#1 Post by DonSchaeffer » Thu Jun 25, 2020 11:37 pm

When observing liquid subjects, I noticed that after a few minutes all the organisms and flotsam starting streaming out of the cover glass. I bought a small circular spirit level and put it on the stage of the microscope. I was able to add shims under the microscope to make it essentially perfectly level. The tilt was very slight, but it makes a huge difference to level it out.

Hobbyst46
Posts: 2927
Joined: Mon Aug 21, 2017 9:02 pm

Re: Levelling My Microscope

#2 Post by Hobbyst46 » Fri Jun 26, 2020 8:10 am

Could it be that the amount of water in your wet-mount slides is excessive ? it should just fill the space between the two glass surfaces, at minimum thickness.

When the amount of water between the slide and coverslip is just sufficient, the factors that affect motion of the water are mainly evaporation and surface tension. Gravity has little, if any, influence. In old microscopes, the arm was connected to the base by means of a rotation axis, hence the slide could be tilted by many degrees, to serve several purposes. They did not have any attached level (AFAIK).

To inhibit evaporation, the rims of the coverslip can be temporarily sealed with petroleum jelly.
Zeiss Standard GFL+Canon EOS-M10, Olympus VMZ stereo

DonSchaeffer
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Joined: Sun Mar 22, 2020 10:06 am
Location: Winnipeg, Manitoba, Canada

Re: Levelling My Microscope

#3 Post by DonSchaeffer » Fri Jun 26, 2020 10:40 am

Those are things I never thought of. Thanks. I'm going to buy some jelly.

deBult
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Joined: Tue Jul 16, 2019 3:20 pm

Re: Levelling My Microscope

#4 Post by deBult » Sat Jun 27, 2020 12:24 am

Don,

Please refer to viewtopic.php?f=4&t=9693#p82554 scroll down to the description of the micro aquarium.

BramHuntingNematodes
Posts: 495
Joined: Tue Jan 21, 2020 1:29 am
Location: Georgia, USA

Re: Levelling My Microscope

#5 Post by BramHuntingNematodes » Sat Jun 27, 2020 3:01 am

Incredible! I might try that as well.
1942 Bausch and Lomb Series T Dynoptic, Custom Illumination

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