What equipment do you use? Post pictures and descriptions of your microscope(s) here!
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Hi, basically I have built my own setup to customize the observing and capturing to suit my needs. I have separated the light source from the microscope to allow more space for a more flexible light source as well as to prevent heat travelling up the microscope and causing evaporation on the slide. The light source is a 7W, 4000k LED light source. In front of the light source is a polarizer that can turn in 360 degrees as required to provide polarized light thru normal light. The microscope has a 45 degree prism on the bottom that allows the light from the light source to be reflected vertically through the microscope objective. This 45 degree prism is slightly offset to give a 3D perspective to the specimen that is being observed (called oblique illumination) which I personally prefer (see example file from a specimen found in some pond water, please note that the detail is very poor due to resizing and taking this from a frame in a video that I took today to add as an example). I am using mostly Nikon objectives with some Amscope Plan objectives when required. The eye pieces are Olympus. I built a switch to shift the prism so that all the light can go to the Canon 200D camera and not be shared with the eye pieces. The frame of the microscope is Brunel. Also built an Analyzer for the Polarizer with a lever for pushing in and out. To the side you can see a motor with a tube and a super magnet on the end. The fine focuser also has a super magnet, this makes coupling easy for doing stack photography rather than wasting time with gears that just cause unnecessary vibration, it is simple and it works well. This setup may look simple but I have spent many hours playing with lenses, lights and optics to get this far, but it truly is a satisfying hobby.
- using a Canon 200D to photograph pond life
- capturing_photographs.jpg (107.07 KiB) Viewed 1296 times
- example from a frame from a video
- example.jpg (48.83 KiB) Viewed 1306 times
- Microscope Base
- microscope_not_in_use.jpg (147.45 KiB) Viewed 1306 times
- Microscope Build
- microscope_parts_description.jpg (159.43 KiB) Viewed 1306 times
Cool. But what tools and things should one have in that drawer? And what's in the hollow space left and right of the drawer? These are the important things...
Someone has been posting the lamp and flash combo. Why have a flash though? If the lamp is set to the right intensity and CCD sensor is giving good data, isn't that a reproducible way to make images? Is there some iso setting that works better -- but then normally that would mean less light not more like a flash. SO I must be missing something here.
Yes, all my tools and other objectives, they are important as you can see...microb wrote: ↑Sun Jul 26, 2020 5:54 pmCool. But what tools and things should one have in that drawer? And what's in the hollow space left and right of the drawer? These are the important things...
the wiring is all in the hidden spaces.
- drawer.jpg (109.31 KiB) Viewed 1237 times
microb wrote: ↑Sun Jul 26, 2020 6:22 pmSomeone has been posting the lamp and flash combo. Why have a flash though? If the lamp is set to the right intensity and CCD sensor is giving good data, isn't that a reproducible way to make images? Is there some iso setting that works better -- but then normally that would mean less light not more like a flash. SO I must be missing something here.
Yes, good idea! I did design it so that the prism could rotate 180 degrees for a second LED light source but I always wondered if it was possible to rather trigger a flash through the polarizer when required while leaving the observation light active.
Attached a stacked photograph of a jumping spider (horribly reduced in size), it does release the camera and move the stepper motor. I pass a "space" key press to the Canon live view, that triggers the camera. This is done using the Arduino.
- Jumping Spider
- jumping spider.jpg (86.94 KiB) Viewed 1231 times